The Sense of the Sentence as an Invariant

Hattori, Shirô

doi:10.1075/z.12.09hat

Cited by 2 publications

(3 citation statements)

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“…The production of NO by J774 macrophages following activation with LPS was reflected by the accumulation of nitrite in the culture medium (12,13). CHX inhibited the accumulation of nitrite by LPS in J774 macrophages, which demonstrates that protein synthesis is a prerequisite for NO formation.…”

Section: Discussionmentioning

confidence: 99%

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Effect of cycloheximide on the expression of LPS‐inducible iNOS, IFN‐β, and IRF‐1 genes in J774 macrophages

et al. 1996

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The effect of cycloheximide (CHX) on the gene expression for inducible NO synthase (iNOS), interferon (IFN)-~ and IFN regulatory factor (IRF)-I was examined in LPSstimulated J774 macrophages. LPS caused increased expression of mRNAs specific for iNOS, IFN-I~, and IRF-1 with different kinetics. Addition of CHX resulted in inhibition of the LPSinduced iNOS gene expression and parallel decrease in NO production. In contrast, expression of IFN-I~ and IRF-1 genes in response to LPS was potentiated in the presence of CHX. These results indicate that de novo protein synthesis is not required for IFN-13 and IRF-1 gene expression and that ongoing protein synthesis including IFN-13 and IRF-1 may be involved in the induction process of iNOS in mouse macrophages.We report here that J774 macrophages respond to LPS treatment by increased expression of mRNAs specific for IFN-I~, IRF-1 and iNOS with the kinetics unique for each gene. Addition of protein synthesis inhibitor cycloheximide (CHX) inhibits the LPS-induced iNOS gene expression while expression of IFN-fl and IRF-1 genes in response to LPS is clearly increased in the presence of CHX. These findings may suggest that LPS acts to induce iNOS via synthesis or release of intermediary cytokines and/or regulatory proteins in J774 macrophages. MATERIALS AND METHODSCell culture: The mouse macrophage cell line J774 was cultured and prepared as described previously (12). J774 macrophages were seeded into 96-well plates for nitrite assay and cytotoxic assay and into 10-era dishes for RNA preparation. To induce NO synthase in the cells, fresh culture medium containing LPS (E. coli, serotype No. 0127: B8, Sigma Chemical Co., Saint Louis, MO, USA)) was added at a concentration of 1 yg/ml. To assess the effects of cyeloheximide (CHX: 0.01 -10/~g/ml, Sigma Chemical Co.), the agent was added to the cells simultaneously with LPS except time-cx)urse study of CHX addition.

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Section: Discussionmentioning

confidence: 99%

“…Nitrite production: Nitrite production, an indicator of NO synthesis, was measured in the supernatant of J774 macrophages, as previously described (12). Nitrite was quantified colorimetrically after adding 100 yl of Griess regent (1% sulfanilamide and 0.1% naphthylenediamide in 5% phosphoric acid) to 100 yl samples.…”

Section: Introductionmentioning

confidence: 99%

Effect of cycloheximide on the expression of LPS‐inducible iNOS, IFN‐β, and IRF‐1 genes in J774 macrophages

et al. 1996

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“…Cytotoxicity to macrophages test [18] J774 Macrophages were seeded (70,000 cells/well) in 96-well flatbottom microplates (Nunclon) with 200 µl of RPMI 1640 medium (Sigma) supplemented with 20 % heat-inactivated fetal calf serum. Cells were allowed to attach for 24 h in a humidified 5 % CO 2 / 95% air atmosphere at 37°C.…”

Section: Trichomonacidal In-vitro Testmentioning

confidence: 99%

Synthesis and Antiparasitic Properties of New 4‐N‐Benzylamino‐4‐Hetarylbut‐1‐enes

Kouznetsov

Castro

Puentes

et al. 2005

Archiv der Pharmazie

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New derivatives of 4-N-benzylamino-4-hetarylbut-1-ene containing a pyridyl nucleus were synthesized from benzylamines and pyridine aldehydes. N-oxide derivatives were obtained from these homoallylamines. Study of the antiparasitic properties of obtained pyridine derivatives as well as of four related benzazepines previously described, was carried out using cytotoxicity assays against Trichomonas vaginalis and epimastigote form of Trypanosoma cruzi protozoa. Compounds showing activity against epimastigote T. cruzi were tested against the amastigote form; unspecific cytotoxicity against macrophages was also studied.

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The Sense of the Sentence as an Invariant

Cited by 2 publications

References 0 publications

Effect of cycloheximide on the expression of LPS‐inducible iNOS, IFN‐β, and IRF‐1 genes in J774 macrophages

Effect of cycloheximide on the expression of LPS‐inducible iNOS, IFN‐β, and IRF‐1 genes in J774 macrophages

Synthesis and Antiparasitic Properties of New 4‐N‐Benzylamino‐4‐Hetarylbut‐1‐enes

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