The sensitivity of Plasmodium protein synthesis to prokaryotic ribosomal inhibitors

New Tricyclic Alkaloids, Cassiarins G, H, J, and K from Leaves of Cassia siamea

“…13) The antimalarial activity of the isolated compounds was determined by the procedure described by Budimulya et al 14) In brief, stock solutions of the samples were prepared in dimethyl sulfoxide (DMSO) (final DMSO concentrations of <0.5%) and were diluted to the required concentration with complete medium (RPMI 1640 supplemented with 10% human plasma, 25 mM N-(2-hydroxyethyl)piperazine-N′-2-ethanesulfonic acid (HEPES) and 25 mM NaHCO 3 ) until the final concentrations of samples in culture plate wells were 10; 1; 0.1; 0.01; 0.001 μg/ mL. The malarial parasite P. falciparum 3D7 clone was propagated in a 24-well culture plates.…”

Delaumonones A and B, New Antiplasmodial Quassinoids from Laumoniera bruceadelpha

“…18) The antimalarial activity of the isolated compounds was determined by the procedure described by Budimulja et al 19) In brief, stock solutions of the samples were prepared in DMSO (final DMSO concentrations of Ͻ0.5%) and were diluted to the required concentration with complete medium (RPMI 1640 supplemented with 10% human plasma, 25 mM HEPES and 25 mM NaHCO 3 ) until the final concentrations of samples in culture plate wells were 10; 1; 0.1; 0.01; 0.001 mg/ml. The malarial parasite P. falciparum 3D7 clone was propagated in a 24-well culture plates.…”

Constituents of Caesalpinia crista from Indonesia

“…Caesalpinin H (1) Antimalarial Activity Antimalarial activity of the isolated compounds was determined by the previously described procedure by Budimulja et al 16) In brief, each compound were separately dissolved in DMSO to obtain a 10 Ϫ2 M stock and kept at Ϫ20°C until used. The malarial parasite, P. falciparum FCR-3/A2 clone, was propagated in a 24-well culture plate in the presence of wide concentration range of each compound.…”

“…16) All of them displayed different potency activity in a dose dependent manner. Among the newly isolated compounds, caesalpinin K (4) and norcaesalpinin F (10) showed the most potent inhibitory activity with an IC 50 value of 120 and 140 nM (Table 3), respectively, and were less than that reported for a well known antimalarial drug, chloroquine (IC 50 , 283-291 nM).…”