The sensor kinase KdpD and the response regulator KdpE control expression of the kdpFABC operon in Escherichia coli

Altendorf, Karlheinz; Voelkner, Petra; Puppe, Wolfram

doi:10.1016/0923-2508(94)90084-1

Cited by 35 publications

(30 citation statements)

References 35 publications

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“…The KdpA subunit is responsible for binding and translocation of K"*", KdpB is the ATPase subunit for energy supply, and KdpF and KdpC are important for stability and assembly of the complex, respectively (15). The expression of the KdpFABC operon is stimulated under K"*" limitation and osmotic stress by the two-component response system KdpD-KdpE (5,89). In the current model KdpD is proposed to sense and convert three intracellular stimuli that depend on K"*" availability or osmotic stress: the f K"*" concentration, the ionic strength, and the ATP concentration (71).…”

Section: Osmotic Stress Response Mechanisms In E Colimentioning

confidence: 99%

An Overview of Molecular Stress Response Mechanisms in Escherichia coli Contributing to Survival of Shiga Toxin-Producing Escherichia coli during Raw Milk Cheese Production

Peng

Tasara

Hummerjohann

et al. 2011

Journal of Food Protection

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The ability of foodborne pathogens to survive in certain foods mainly depends on stress response mechanisms. Insight into molecular properties enabling pathogenic bacteria to survive in food is valuable for improvement of the control of pathogens during food processing. Raw milk cheeses are a potential source for human infections with Shiga toxin-producing Escherichia coli (STEC). In this review, we focused on the stress response mechanisms important for allowing STEC to survive raw milk cheese production processes. The major components and regulation pathways for general, acid, osmotic, and heat shock stress responses in E. coli and the implications of these responses for the survival of STEC in raw milk cheeses are discussed.

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Section: Osmotic Stress Response Mechanisms In E Colimentioning

confidence: 99%

An Overview of Molecular Stress Response Mechanisms in Escherichia coli Contributing to Survival of Shiga Toxin-Producing Escherichia coli during Raw Milk Cheese Production

Peng

Tasara

Hummerjohann

et al. 2011

Journal of Food Protection

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“…In E. coli , the primary response to environmental osmotic stress is the regulation of potassium content in the cytoplasm through the kdpFABC and kdpDE operons (Walderhaug et al ., 1992). The kdpFABC operon is induced by high osmolarity and encodes the four‐subunit, K + ‐transporting P‐type ATPase comprising the KdpA, KdpB, KdpC and KdpF proteins (Walderhaug et al ., 1992; Altendorf et al ., 1994). Thus, when bacteria sense a change in external osmotic conditions, turgor pressure is maintained by adjusting the osmotically active components in their cytoplasm, e.g.…”

Section: Introductionmentioning

confidence: 99%

Interaction of the sensor module of Mycobacterium tuberculosis H37Rv KdpD with members of the Lpr family

Steyn¹,

Joseph

Bloom

2003

Molecular Microbiology

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The genetic and biochemical mechanisms by which Mycobacterium tuberculosis senses and responds to the complex environment that it encounters during infection and persistence within the host remain unknown. In a number of bacterial species, the Kdp signal transduction pathway appears to be the primary response to environmental osmotic stress, which is primarily mediated by K+ concentration in bacteria. We show that kdp encodes for components of a mycobacterial signalling pathway by demonstrating the K+ dependence of kdpFABC expression in both M. tuberculosis H37Rv and Mycobacterium smegmatis. To identify proteins of M. tuberculosis that participate in this signalling pathway, we used the N-terminal sensing module of the histidine kinase KdpD as bait in a yeast two-hybrid screen. We show that the sensing domain of KdpD interacts specifically with two membrane lipoproteins, LprJ (Rv1690) and LprF (Rv1368). Overexpression of lprF and lprJ alleles in mycobacterial kdpF-lacZ reporter strains enabled us to identify alleles that modulate kdpFABC expression. By exploiting the yeast three-hybrid system, we have found that the histidine kinase domain of KdpD forms ternary complexes with LprF and LprJ and the sensing module of KdpD. Our results establish a role for membrane proteins in the Kdp signalling pathway and suggest that LprF and LprJ function as accessory or ligand-binding proteins that communicate directly with the sensing domain of KdpD to modulate kdp expression.

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“…In this study, we found that three genes of the kdp operon (kdpABC) were induced in biofilms by comparison to both planktonic growth conditions (Table 5) and two other genes (kdpDE) were induced by comparison to the exponential growth phase (Table 3). In E. coli, the Kdp system is composed of the ion motive P-type ATPase encoded by the kdpFABC operon, and expression of the kdpFABC operon is regulated by an adjacent operon, kdpDE (2). KdpD is a membrane-spanning sensor kinase, and KdpE is a cytosolic transcriptional activator.…”

Section: Vol 186 2004 Global Gene Expression In S Aureus Biofilms mentioning

confidence: 99%

Global Gene Expression in Staphylococcus aureus Biofilms

et al. 2004

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We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71: [4206][4207][4208][4209][4210][4211] 2003). In this report, we used a murine model of catheter-based biofilm formation to demonstrate that a UAMS-1 sarA mutant also has a reduced capacity to form a biofilm in vivo. Surprisingly, mutation of the UAMS-1 ica locus had little impact on biofilm formation in vitro or in vivo. In an effort to identify additional loci that might be relevant to biofilm formation and/or the adaptive response required for persistence of S. aureus within a biofilm, we isolated total cellular RNA from UAMS-1 harvested from a biofilm grown in a flow cell and compared the transcriptional profile of this RNA to RNA isolated from both exponential-and stationary-phase planktonic cultures. Comparisons were done using a custom-made Affymetrix GeneChip representing the genomic complement of six strains of S. aureus (COL, N315, Mu50, NCTC 8325, EMRSA-16 [strain 252], and MSSA-476). The results confirm that the sessile lifestyle associated with persistence within a biofilm is distinct by comparison to the lifestyles of both the exponential and postexponential phases of planktonic culture. Indeed, we identified 48 genes in which expression was induced at least twofold in biofilms over expression under both planktonic conditions. Similarly, we identified 84 genes in which expression was repressed by a factor of at least 2 compared to expression under both planktonic conditions. A primary theme that emerged from the analysis of these genes is that persistence within a biofilm requires an adaptive response that limits the deleterious effects of the reduced pH associated with anaerobic growth conditions.

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The sensor kinase KdpD and the response regulator KdpE control expression of the kdpFABC operon in Escherichia coli

Cited by 35 publications

References 35 publications

An Overview of Molecular Stress Response Mechanisms in Escherichia coli Contributing to Survival of Shiga Toxin-Producing Escherichia coli during Raw Milk Cheese Production

An Overview of Molecular Stress Response Mechanisms in Escherichia coli Contributing to Survival of Shiga Toxin-Producing Escherichia coli during Raw Milk Cheese Production

Interaction of the sensor module of Mycobacterium tuberculosis H37Rv KdpD with members of the Lpr family

Global Gene Expression in Staphylococcus aureus Biofilms

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