The separation of human serum IgG into subclass fractions by immunoaffinity chromatography and assessment of specific antibody activity

Bird, P; Lowe, J; Stokes, R P; Bird, AngusGraham; Ling, N. R.; Jefferis, Roy

doi:10.1016/0022-1759(84)90209-6

Cited by 41 publications

(10 citation statements)

References 7 publications

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“…In the present investigation, all subclasses showed some response to the protein toxin. This is in agreement with other studies in which IgGl, lgG4 and or IgG3 predominated in frequency and levels of response to tetanus toxoid immunization, although some studies reported an absence of a significant IgG2 response (4,38,43). IgGl was the dominant IgG subclass, comprising 62% of the response in the nonhuman primates.…”

Section: Discussionsupporting

confidence: 93%

Evidence for serum immunoglobulin G (IgG) antibody responses in Macaca fascicularis identified by monoclonal antibodies to human IgG subclasses

Ebersole¹,

Bauman²,

O'Dell³

et al. 1997

Oral Microbiology and Immunology

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This investigation determined the capacity of murine monoclonal antibodies directed to human immunoglobulin G (IgG) subclasses to identify molecules with conserved epitopes in the serum of the nonhuman primate, Macaca fascicularis. We subsequently utilized this cross-reactivity to document the characteristics of IgG subclass antibody responses in M. fascicularis to parenteral immunization with intact oral microorganisms, antigens from oral microorganisms, and finally a defined protein toxin, tetanus toxoid. The IgG response in nonhuman primates immunized with tetanus toxoid showed a 40-fold and 110-fold increase after primary and secondary immunizations, respectively. The major IgG subclass responses were IgG1 and IgG3, with little, though significant, responses in the IgG4 and IgG2 subclasses. Seventy-five to 94% of the natural IgG antibody in nonhuman primate sera to Porphyromonas gingivalis, Prevotella intermedia and Campylobacter rectus was IgG1. IgG2 and IgG3 predominated to Bacteroides fragilis, IgG4 to Actinomyces viscosus and an equal distribution among the subclasses was noted in response to Fusobacterium nucleatum. Parenteral immunization of nonhuman primates with intact P. gingivalis elicited primarily IgG3 and IgG4, while the post-immunization IgG response to P. intermedia was largely IgG1. Nonhuman primates were also parenterally immunized with cell envelope antigens of P. gingivalis, P. intermedia, or a combination of cell envelope antigen from C. rectus and F. nucleatum and cell wall antigens of A. viscosus. The greatest IgG antibody response seen post-immunization was reactive with anti-human IgG1 for all of these antigens except to C. rectus which bound nonhuman primate antibody reactive with anti-human IgG2. It appears that the bacteria and their products exhibit unique differences in their induction of serum IgG subclass antibody responses. The characteristics of their immunogenicity as detected by the nonhuman primate may contribute to the ability of the immune responses to effectively interact with these pathogens.

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Section: Discussionsupporting

confidence: 93%

Evidence for serum immunoglobulin G (IgG) antibody responses in Macaca fascicularis identified by monoclonal antibodies to human IgG subclasses

Ebersole¹,

Bauman²,

O'Dell³

et al. 1997

Oral Microbiology and Immunology

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“…The results of the present study indicate that the IgG antibodies formed after booster vaccination with tetanus toxoid are predominantly of the IgG1 subclass, both in healthy controls and in HIVinfected individuals. This is compatible with the fact that tetanus toxoid, being a protein antigen, induces a T-celldependent immune response which consists mainly of IgG1 in healthy adults (2,11) as well as in HIV-infected individuals (1). The present study extends our earlier observations and demonstrates that HIV-infected individuals with fewer than 200 CD4 ϩ T lymphocytes exhibit a quantitatively impaired IgG1 anti-tetanus toxoid response.…”

Section: Discussionsupporting

confidence: 61%

Immunoglobulin G (IgG) Subclass Distribution and IgG1 Avidity of Antibodies in Human Immunodeficiency Virus-Infected Individuals after Revaccination with Tetanus Toxoid

Kroon

Tol

Zijde

et al. 1999

Clin Diagn Lab Immunol

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In human immunodeficiency virus (HIV)-infected individuals the amount of antibodies formed after vaccination with T-cell-dependent recall antigens such as tetanus toxoid is proportional to the peripheral blood CD4+ T-lymphocyte counts. To investigate whether the immunoglobulin G (IgG) subclass distribution and avidity of the antibodies produced after vaccination are affected as well, we gave 13 HIV-infected adults with low CD4+ T-lymphocyte counts (<200 × 106/liter; group I), 11 HIV-infected adults with intermediate CD4+ T-lymphocyte counts (≥200 × 106/liter; group II), and 5 healthy controls booster immunizations with tetanus toxoid. The prevaccination antibody concentrations against tetanus toxoid were similar in the HIV-infected and healthy adults. After vaccination the total IgG and the IgG1 anti-tetanus toxoid antibody concentrations were significantly lower in group I than in group II and the controls. The avidity of the IgG1 anti-tetanus toxoid antibodies formed by HIV-infected adults was within the range for healthy controls, irrespective of their CD4+T-lymphocyte counts.

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“…After immunization of rats with GM1 ganglioside, a persistent component of the antibody response is IgM [Pestronk, unpublished observations), as in the antibodies from ALS patients. T-independent B lymphocyte responses, ones that might be expected to mediate humoral responses to the carbohydrate groups on gangliosides, would be expected to produce mainly IgM and IgG2 types of antibodies [17][18][19]. Perhaps antiganglioside antibodies are produced through this or another specialized subgroup of B-cell functions.…”

Section: Discussionmentioning

confidence: 99%

Patterns of serum IgM antibodies to GM1 and GD1a gangliosides in amyotrophic lateral sclerosis

Pestronk¹,

Adams²,

Cornblath³

et al. 1989

Annals of Neurology

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We studied the incidence and clinical correlates of serum antibodies to GM1 and GD1a gangliosides in patients with classical amyotrophic lateral sclerosis (ALS) and other "motor nerve" syndromes. Serum antibodies to GM1 and GD1a gangliosides were measured using enzyme-linked immunosorbent assays. Our results showed that polyclonal immunoglobulin M (IgM) antibodies to the GM1 or GD1a ganglioside or both were present at serum dilutions of 1:25 to 1:4,000 in 78% (57/73) of patients with ALS. Only 8% of normal controls had similar antibodies. The pattern of serum antibody reactivity correlated with the pattern of clinical involvement in our patients. Selective reactivity to GD1a ganglioside was common when upper motor neuron signs were prominent. IgM reactivity to GM1 ganglioside was common in ALS patients with prominent lower motor neuron signs. Most patients with motor neuropathies had serum reactivity to both GM1 and GD1a gangliosides. These results provide further evidence of ongoing autoimmune processes in ALS patients. There is a strong relationship between serum antiganglioside antibodies and patterns of clinical involvement in ALS.

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The separation of human serum IgG into subclass fractions by immunoaffinity chromatography and assessment of specific antibody activity

Cited by 41 publications

References 7 publications

Evidence for serum immunoglobulin G (IgG) antibody responses in Macaca fascicularis identified by monoclonal antibodies to human IgG subclasses

Evidence for serum immunoglobulin G (IgG) antibody responses in Macaca fascicularis identified by monoclonal antibodies to human IgG subclasses

Immunoglobulin G (IgG) Subclass Distribution and IgG1 Avidity of Antibodies in Human Immunodeficiency Virus-Infected Individuals after Revaccination with Tetanus Toxoid

Patterns of serum IgM antibodies to GM1 and GD1a gangliosides in amyotrophic lateral sclerosis

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