The Sequence of Changes in the Biosynthesis of Sulfated Glycosaminoglycans in Acute, Experimental Liver Disease

Gressner, A. M.; Heinrigs, Sylvia; Grouls, Peter

doi:10.1515/cclm.1982.20.1.15

Cited by 15 publications

(20 citation statements)

References 31 publications

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“…. 1982, Both liver fibrosis and cir rhosis were produced in that way [Gressner et al, 1977a[Gressner et al, , 1982. Given intraperitoneally, the doses induc ing acute liver injury in rats vary between 50 and 100 mg ThAA per kilogram body weight [Gressner et al, 1977a[Gressner et al, , 1980.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Experimental Liver Injury on Rat Blood and Alveolar Bone under Stress

Lassila¹,

Virtanen²

1984

Cells Tissues Organs

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Experimental liver injury was induced to test rats with a daily injection of thioacetamide (ThAA). The doses used for intraperitoneal administrations were 50 mg/kg body weight. The loss of body weight in the 3-week test period was 15%. In the liver there was seen progressive changes which displayed cell necrosis and regeneration. The influence of ThAA on rat blood and serum was checked using standard biochemical assays consisting of the percentage of blood obtainable and the serum/blood ratio and analysis of serum alanine transferase, serum alkaline phosphatase, serum creatinine, and hydroxyproline in the acute, subacute, chronic, and necrotic stage of liver injury. With ThAA injections, the stimulated rate of glycosaminoglycan synthesis had its association to the serum calcium content. It decreased continuously as function of traumatization time. In the 3-week test period, histological findings show in the alveolar bone, around the teeth, when under occlusal stress and ThAA traumatization, the distinct decrease in osteoblastic activity and less osteoids indicating thus the decreased formation of new bone. Conspicuous osteoclastic resorption was also seen in the same area.

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Section: Introductionmentioning

confidence: 99%

“…The doses used have verified effectively the increase rate of the synthesis of acid glycosaminoglycans (GAGs), except dermatan sulfate, both in vivo [Gressner et al, 1977a[Gressner et al, . 1980[Gressner et al, , 1982 and in vitro [Gressner et al, 1982],…”

Section: Introductionmentioning

confidence: 99%

Influence of Experimental Liver Injury on Rat Blood and Alveolar Bone under Stress

Lassila¹,

Virtanen²

1984

Cells Tissues Organs

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“…Liver injury due to GalN influences the synthesis rate of the glycan moiety of glycoproteins [Gressner et al, 1981[Gressner et al, . 1982Monnetelal.. 1985].…”

Section: Discussionmentioning

confidence: 99%

“…Both an increased livercollagen content and an increased and changed acid glycosaminoglycan content are typical in liver cirrhosis [Murata et al. 1984], A strong relative increase in the production of chondroitin sulfate and dermatan sulfate is most probable [Gressner et al. 1982;Murata et al, 1984].…”

Section: Discussionmentioning

confidence: 99%

Regeneration of D-Galactosamine-Traumatized Rat Liver with Natural Protoberberine Alkaloids from <i>Enantia chloranth</i><i>a</i>

Virtanen

Lassila²,

Njimi³

et al. 1988

Cells Tissues Organs

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Experimental liver injury was provoked in test rats with intraperitoneal injections of D-galactosamine. Traumatized rats received further intraperitoneal injections of Hepasor, a protoberberine alkaloid mixture from Enantia chlorantha (Annonaceae). Biochemical assays from blood plasma, serum alanine transferase, serum alkaline phosphatase, serum creatinine, serum hydroxyproline and serum calcium were done and liver and kidney samples for histological processing were taken. The biochemical results obtained indicate a marked influence by Hepasor on the serum alanine transferase activities and serum hydroxyproline values in female rats, but more accidental ones in male rats. A reduction in serum alkaline phosphatase activity and the serum creatinine values was also found, being also dependent on sex. The histological findings in the liver sections of female rats show that 1 week Hepasor therapy greatly furthers the healing process in a D-galactosamine-pretraumatized liver, eliminating megalocytosis, contraction of chromatines and other disorders in the cell architecture. The inhibitory effect of Hepasor on proceeded traumatization caused by D-galactosamine was nearly complete.

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“…These changes occur chiefly in the hyaluronate, chondroitin, sulphate and dermatan sulphate fractions. The extensive accumulation of intercellular glycosaminoglycans in cirrhotic and fibrotic liver tissue is mainly ascribed to active mechanisms like enhanced synthesis (9)(10)(11)(12), but the knowledge of contributory mechanisms is scanty. Passive processes like the deposition of extrahepatically synthesized proteoglycans in the liver (13,14) and/or Inhibition of hepatic glycosaminoglycan degradation by reduced lysosomal enzyme activities in chronically injured liver tissue are possible, but äs yet experimentally unproven.…”

Section: Introductionmentioning

confidence: 99%

Changes in the Catalytic Activities of Proteoglycan-Degrading Lysosomal Enzymes in Parenchymal and Non-Parenchymal Liver Cells and in Serum During the Development of Experimental Liver Fibrosis

Weber¹,

Kehrer²,

Gressner³

et al. 1983

CCLM

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Summary:The catalytic activities of 4 glycosidases (hyaluronate-4-glycanohydrolase (EC 3.2.1.35), ß-Nacetyl-D-glucosaminidase (EC 3.2.1.30), ß-glucuronidase (EC 3.2.1.31), -L-iduronidase (EC 3.2.1.76)), of the arylsulphatases A and B (EC 3.1.6.1) and of the protease cathepsin D (EC 3.4.23.5) were measured in extracts from hepatocytes and non-parenchymal cells and in serum during the development of thioacetamideinduced rat liver fibrosis (22 weeks). In non-parenchymal liver cells the catalytic activities of ß-N-acetyl-Dglucosaminidase, ß-glucuronidase, -L-iduronidase and cathepsin D were increased significantly during chronic liver damage, but that of hyaluronate-4-glycanohydrolase was reduced by 40 to 65% during the period of applicätion of thioacetamide. The catalytic activities of the arylsulphatases were lowered by 65% compared to control values in the 12th week but with advancing liver damage the catalytic activities returned to nearly normal values. Parenchymal cells of rats, which had been liver-damaged for 6 months, contained strongly elevated activities of ß-glucuronidase, ß-N-acetyl-D-glucosaminidase, arylsulphatases A and B, and cathepsin D but only slightly increased activities of hyaluronate-4-glycanohydrolase and -L-iduronidase, respectively. In the serum of liver-damaged rats the activity of -L-iduronidase was strongly elevated, while that of Nacetyl-ß-D-glucosaminidase was only slightly increased. The activities of ß-glucuronidase and of arylsulphatases A and B were decreased during the whole period of treatment. The catalytic functions of hyaluronate-4-glycanohydrolase and of cathepsin D, respectively, were decreased initially, but both enzyme activities were elevated during the more advanced stages of long term thioacetamide treatment. Aktivitätsänderungen Proteoglykan^abbauender^ lysosomaler Enzyme in Parenchym-und Nicht-Parenchymzellen der Leber sowie im Serum während der Entwicklung einer experimentellen LeberfibroseZusammenfassung: Die katalytischen Aktivitäten von vier Glykosidasen (Hyaluronat-4-glykanohydrolase (EC 3.2.1.35), ß-N-Acetyl-D-glucosaminidase (EC 3.2.1.30), ß-Glucuronidase (EC 3.2.1.31), cc-LIduronidase (EC 3.2,1.76)) sowie zweier Sulfatasen (Arylsulfatase A und B) (EC 3.1.6.1) und einer Protease /(Kathepsin D) (EC 3.4.23.5) wurden in den löslichen Extrakten von Hepatocyten und Nicht-Parenchymzellen der Leber und im Serum im Verlaufe einer Thioacetamid-induzierten Leberfibrose (22 Wochen) bestimmt. In Nicht-Parenchymzellen sind die katalytischen Aktivitäten der ß-N-Acetyl-£>-glucosaminidase, ß-Glucuronidase, -L-Idufonidase und Kathepsin D bei chronischer Leberschädigung signifikant erhöht, wohingegen die katalytische Aktivität der Hyaluronat-4-glykanohydrolase im gesamten Untersuchungszeitraum um 40 bis 65% vermindert ist. Die katalytischen Aktivitäten der Arylsulfatasen A und B waren in der 12. Woche der Schädigung um 65% erniedrigt (verglichen mit den Kontrollen), mit fortschreitender Leberschädigung wurden jedoch wieder Normalwerte erreicht. Parenchymzellen von Ratten, die 6...

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The Sequence of Changes in the Biosynthesis of Sulfated Glycosaminoglycans in Acute, Experimental Liver Disease

Cited by 15 publications

References 31 publications

Influence of Experimental Liver Injury on Rat Blood and Alveolar Bone under Stress

Influence of Experimental Liver Injury on Rat Blood and Alveolar Bone under Stress

Regeneration of D-Galactosamine-Traumatized Rat Liver with Natural Protoberberine Alkaloids from <i>Enantia chloranth</i><i>a</i>

Changes in the Catalytic Activities of Proteoglycan-Degrading Lysosomal Enzymes in Parenchymal and Non-Parenchymal Liver Cells and in Serum During the Development of Experimental Liver Fibrosis

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