The sequential appearance of Ia-like antigens and two different complement receptors during the maturation of human neutrophils.

Abstract

The differentiation of neutrophils in normal, abnormal, and leukemic disease states has been extensively characterized from the aspects of morphology, cytochemistry, and functional properties. However, in comparison to the many studies of membrane components as indices of differentiation in the lymphoid system, little attention has been given to analysis of the membrane structural changes that occur during granulocyte differentiation. Since the acquisition of receptors and other mature cell membrane components is closely associated with functional differentiation, it is likely that identification of the structures and receptors associated with membrane differentiation would provide additional perspectives of particular utility in studies of granulocyte physiology, as well as assistance in classification of certain abnormal disease states.Mature neutrophils have complement (C) ~ receptors that are believed to be exclusively CR1 (immune adherence, C4b-C3b receptors) but not CI~ (C3d receptors) (1-4). However, there have been no studies of the specificity of neutrophil C receptors for different fragments of C3 or C4 molecules, or of when C receptors appear during neutrophil maturation. By contrast, the specificity * Supported by grants from the National Cancer Institute (CA-16190 and CA-20107) and the National Institute for Heart and Lung Disease (HL-18828) DHEW.$ An Irma T. Hirschl Career Scientist. § An Andrew W. Mellon Teacher-Scientist. II Recipient of a Research Career Development Award from the National Institutes of Health (K04-AI-00216).Abbreviations used in this paper: AML, acute myelogenous leukemia: B lymphocyte or B cell, bone marrow-derived lymphocyte; T lymphocyte or T cell, thymus-derived lymphocyte; BSA, bovine serum albumin; band or band form, neutrophil maturation stage proceeding the polymorphonuclear stage; C, complement; CR1, complement receptor type one, the immune adherence (C4b-C3b) receptor; CI~, complement receptor type two, C3d receptor; C4b, the 204,000 dalton fragment of C4 generated by cleavage of C4 by Ci; C3b, the 181,000-dalton fragment of C3 generated by cleavage of C3 by C4-~ or trypsin; C3c (140,000 daltens) and C3d (30,000 daltons) fragments are the fragments of C3b generated by cleavage with C3b inactivator, trypsin, or elastase; CML, chronic myelogenous leukemia; EAC, sheep erythrocyte-rabbit IgM antibodycomplement complexes; Fc receptor, receptor for the Fc portion of IgG; HBSS, Hanks' balanced salt solution; Ia antigens, human analogues of murine "immunity associated" antigens; polymorphonuclear neutrophil, neutrophil with segmented nucleus; PBS, phosphate-buffered saline; PBS/ BSA, PBS containing 1% BSA and 0.2% NAN,; SDS, sodium dodecyl sulfate.

High and low Fc IgG-receptor expression in human chronic granulocytic leukaemia cells

“…In normal leucocytes and in some cases of "highreceptor" myeloid leukaemias, the amount of CR2+ cells in the 1P105 fraction was significantly reduced. These observations are consistent with the data of Ross et al (1978) indicating that less dense neutrophils, containing both CR1 and CR2 receptors, differed in the mechanism of EAC-rosette formation from their denser counterparts.…”

“…The leucocyte suspension were then separated into fractions by discontinuous density-gradient centrifugation according to Ross et al (1978), slightly modified (Harlozin '-ska et al, 1980 Stingl & Knapp (1977) as earlier described in detail (Harlozinska et al, 1980). Cells with C3 receptors were detected by their capacity to form rosettes with SRBC coated with 19S anti-SRBC antibody and complement (EAC rosettes) (Gupta et al, 1976).…”

“…In a previous study we have observed that AML blasts are poor in receptors (Harlozinska et al, 1980). The number of cell-surface markers increases with differentiation Ross et al, 1978; Berridge, 1979;Harlozinska et al, 1980).…”

Surface properties of bacillus Calmette-Guérin-activated mouse macrophages. Reduced expression of mannose-specific endocytosis, Fc receptors, and antigen F4/80 accompanies induction of Ia.

“…Two hypotheses can be proposed to account for the changes in plasma membrane observed, viz., that the cell population is changed and represents an earlier stage or alternate pathway of macrophage differentiation or that the mature macrophage is able to modulate its surface phenotype. In this regard, it is known that Ia-like antigens may be expressed during earlier stages of myeloid differentiation (32), but that these antigens can also be induced on inflammatory peritoneal macrophages by treatment with lymphokines (11). It remains to be seen whether lymphokines can also induce the loss of F4/80 and other markers in nonactivated macrophages in vitro.…”

Human megakaryocytes. II. Expression of platelet proteins in early marrow megakaryocytes.

“…FcR and Ia antigen have been recognized as differentiation markers of the Blymphoid monocytic, myeloid, and erythroid series (38)(39)(40)(41). Parallel studies of megakaryocytes and small, low density marrow cells disclosed that FcR were found on all mature megakaryocytes and small marrow cells bearing platelet markers, whereas Ia antigen was only detected on a proportion of mature megakaryocytes, but not on any of the megakaryocytic precursors.…”