The sero-prevalence and sero-incidence of African horse sickness and equine encephalosis in selected horse and donkey populations in Zimbabwe

Gordon, Stuart; Bolwell, Charlotte F.; Rogers, Chris W.; Musuka, Godfrey; Kelly, Patrick J.; Guthrie, Alan John; Mellor, P. S.; Hamblin, C.

doi:10.4102/ojvr.v84i1.1445

Cited by 10 publications

(16 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This survey indicated that the majority of Namibian donkeys (63.5%) had been exposed to AHSV. The results of the current study are in agreement with studies from other African countries that have reported donkey AHSV prevalence of between 59.3% and 72% [9,10]. It would appear that all AHSV serotypes, except AHSV-8, occur in Namibia.…”

Section: Discussionsupporting

confidence: 92%

Seroprevalence of African horse sickness in selected donkey populations in Namibia

et al. 2020

View full text Add to dashboard Cite

Background and Aim: African horse sickness (AHS) is a non-contagious viral disease of horses and other equids caused by an arbovirus belonging to the Reoviridae family and genus Orbivirus. AHS is an endemic disease that is responsible for the death of a high number of horses every year in Namibia. At present, there is no information on the prevalence and distribution of AHS virus (AHSV) serotypes in the different regions of Namibia. Therefore, this survey aimed to fill this knowledge gap by investigating the AHSV seroprevalence in Namibian donkeys. Materials and Methods: A total of 260 blood samples (20 samples for each region) were randomly collected from donkeys aged between 3 and 5 years. Sera were screened for AHSV-specific immunoglobulin G antibodies using a commercial competitive enzyme-linked immunosorbent assay kit and samples positive to AHSV antibodies were further tested by serum neutralization (SN) assay to evaluate the AHSV serotype-specific immune response. Results: Seroprevalence of antibodies against AHSV in Namibian donkeys was 63.5%. The AHSV prevalence was significantly higher in the northern region (64%) than in the southern region (36%). A significantly (p<0.05) higher number of donkeys had antibodies against AHSV-6 (37.8%) and AHSV-9 (37.8%). The AHSV-2, AHSV-6, and AHSV-9 prevalence were higher (p<0.05) in the northern regions compared to the southern regions. None of the donkeys in this study, however, tested positive for AHSV-8. Conclusion: Results of the current study indicate that all AHSV serotypes have either circulated previously or are circulating in Namibia except for AHSV-8. In particular, AHSV-1, -2, -3, -4, -5, -6, and -9 serotypes have circulated or are circulating in the northern region of Namibia, while AHSV-1, -4, -5, -6, -7, and -9 have infected donkeys in the south. AHSV-9 and AHSV-6 were the most prevalent serotypes detected in donkeys in this study. SN results showed that several donkeys from Kavango East, Kavango West, and Ohangwena regions had been exposed to multiple serotypes, indicating the possibility of cocirculation of several strains in Namibia.

show abstract

Section: Discussionsupporting

confidence: 92%

Seroprevalence of African horse sickness in selected donkey populations in Namibia

et al. 2020

View full text Add to dashboard Cite

show abstract

“…En Ethiopie, une étude montre ainsi une séroprévalence plus élevée chez les ânes (51,1 %) que chez les chevaux (28,3 %) (Bitew et al, 2011). Nos résultats confortent ceux obtenus par Gordon et al (2017). En général, la séroprévalence importante régulièrement observée chez l'âne confirme le rôle que jouerait cette espèce dans la circulation du virus de la PEA en intervenant comme un réservoir (Hamblin et al, 1998).…”

Section: ■ Discussionunclassified

Risque épidémiologique de la peste équine africaine chez les ânes au Burkina Faso

Savadogo

Sow

Dahourou

et al. 2018

Rev. Elev. Med. Vet. Pays Trop.

View full text Add to dashboard Cite

Le virus de la peste équine africaine (PEA) provoque des épizooties sévères lorsqu’il est transmis aux chevaux par ses vecteurs, les culicoïdes. Une étude transversale a été réalisée dans cinq régions du Burkina Faso afin de déterminer la séroprévalence du virus chez les ânes et d’identifier les facteurs de risque potentiel. Au total, 460 sérums ont été prélevés dans 15 villages. Une analyse par dosage immunoenzymatique (ELISA) de compétition a été réalisée pour détecter les anticorps antivirus PEA. La séroprévalence apparente globale était de 72,6 %, témoignant du fait que les ânes étaient infectés par le virus de la PEA. Une analyse multivariée par régression logistique a été effectuée en vue de déterminer les facteurs affectant le niveau de séroprévalence. Celle-ci est apparue significativement différente entre les sites d’étude, confirmant le rôle de la zone agroécologique dans la survenue de la maladie. L’âge et le poids des animaux ont également eu un effet significatif sur la séroprévalence, contrairement au sexe, à la couleur de la robe et à la taille du troupeau. Cette étude a mis en évidence la circulation active du virus de la peste équine chez les ânes au Burkina Faso.

show abstract

“…The chance of isolating free virus was improved by assaying the blood samples collected before animals seroconverted. Virus isolations were made by intravenous inoculation of 11-day-old embryonated hens’ eggs (IAH, UK) and by intracerebral inoculation of 1- to 2-day-old suckling mice (CD-1, UK) using techniques described by Gordon et al ( 2017 ). The viruses isolated were typed using virus type-specific antisera and BHK-21 cells in microtitre plates using neutralisation test methods modified from Hazrati and Ozawa ( 1965 ).…”

Section: Methodsmentioning

confidence: 99%

A serosurvey of bluetongue and epizootic haemorrhagic disease in a convenience sample of sheep and cattle herds in Zimbabwe

Gordon

Bolwell

Rogers

et al. 2017

Onderstepoort j. vet. res.

Self Cite

View full text Add to dashboard Cite

A convenience sample of sheep and cattle herds around the cities of Harare, Kwekwe and Bulawayo, located in the Highveld region of Zimbabwe, was used to estimate the sero-prevalence and sero-incidence of bluetongue virus (BTV) and epizootic haemorrhagic disease virus (EHDV) antibodies. A competitive enzyme-linked immunosorbent assay was used to identify serum antibodies against BTV and EHDV across three rainy seasons. The median sero-prevalence of BTV and EHDV antibodies in cattle was 62% (interquartile range [IQR]: 30–89) and 56% (IQR: 5–77), respectively. In sheep, the median sero-prevalence of BTV and EHDV was 41% (IQR: 19–63) and 0% (IQR: 0–21), respectively. Median sero-incidences of BTV and EHDV antibodies in cattle of 43% (IQR: 22–67) and 27% (IQR: 9–57) respectively were recorded. The median sero-incidence of BTV in sheep was 14% (IQR: 6–23). Based on these preliminary findings, animal health workers in Zimbabwe should continue to monitor the exposure rates of cattle and sheep to BTV and consider the possibility of strains emerging with increased pathogenicity. There are no previous published reports of antibodies against EHDV in Zimbabwe so the possibility of epizootic haemorrhagic disease existing in domestic livestock should now be considered by Zimbabwean animal health officials. Seroconversions to BTV and EHDV occurred predominantly at the end of each rainy season (March and April), which generally corresponds to high numbers of the Culicoides vectors. BTV isolations were made from three individual cows in two of the sentinel herds and all three were identified as serotype 3. This is the first time BTV serotype 3 has been recorded in Zimbabwe, although its presence in neighbouring South Africa is well documented.

show abstract

The sero-prevalence and sero-incidence of African horse sickness and equine encephalosis in selected horse and donkey populations in Zimbabwe

Cited by 10 publications

References 13 publications

Seroprevalence of African horse sickness in selected donkey populations in Namibia

Seroprevalence of African horse sickness in selected donkey populations in Namibia

Risque épidémiologique de la peste équine africaine chez les ânes au Burkina Faso

A serosurvey of bluetongue and epizootic haemorrhagic disease in a convenience sample of sheep and cattle herds in Zimbabwe

Contact Info

Product

Resources

About