The serological response of adult cattle after vaccination with Brucella abortus strain 19 and RB51

Poester, Fernando Padilla; Ramos, Ernani Tadeu; Gomes, Marcos José Pereira; Chiminazzo, Cláudio; Schurig, Gerhardt G.

doi:10.1590/s1413-95962000000100010

Cited by 11 publications

(10 citation statements)

References 5 publications

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“…These explanations substantiate with the findings that the heifers vaccinated with SRB51 acquired visible immune response four weeks post-immunization, 38 a peak in antibody response in adult cattle vaccinated with RB51 at day 30 by employing an I-ELISA. 39 The antibody titer has been reported to be significantly increased in the vaccinated calves after one month and the titer declined but remained positive up to six months and then negative throughout the 12 months study periods. 23 The antibodies titer began and reached the maximum as early as first week of RB51 vaccination in buffalo calves, remain steady till two weeks post vaccination (WPV), fluctuating till the 6 th WPV, then dropped sharply when it disappeared at 11 WPV till the end of the experiment.…”

Section: Discussionmentioning

confidence: 95%

“…42 However, these findings contradict with the report that the adult cattle, pregnant or not vaccinated once or twice with 2  10 9 viable B. abortus strain RB51 did not seroconvert in the traditional brucellosis tests (Rose Bengal, Serum agglutination and Mercaptoethanol tests), whereas animals vaccinated while pregnant did not abort and no B. abortus was isolated from their vaginal mucus and milk. 39 It has also been reported that the vaccination with RB51 alone is not enough to control brucellosis in endemic areas, and therefore it has suggested eliminating all positive animals at the time of vaccination and all new positive animals after that for long periods of time. 43 It appears from this study that the HIR is produced both in cattle and buffaloes but it is comparatively higher in cattle than buffaloes immunized with B. abortus RB 51 vaccine.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Humoral Immune Responses Between Cattle and Buffaloes Immunized With Commercial Brucella Abortus Strain Rb51 Vaccine in Bangladesh

Mohamud¹,

Mohamud²,

Rahman³

et al. 2020

JVMOHR

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Background: The effective control and eradication of brucellosis can be achieved by rapid and accurate diagnosis and effective vaccination but both have limitations. Therefore, brucellosis research is currently focused on the improvement of the diagnosis and vaccine induced prophylaxis. Moreover, diagnostic tests and immunization have not been thoroughly studied in buffaloes and even not compared with cattle. Therefore, the comparative evaluation of the immunological responses of Brucella vaccinated cattle and buffaloes would be required for both the diagnosis and vaccine induced efficacy. Objectives: The main objective of this study was to compare the humoral immune response (HIR) between cattle and buffalo cows immunized with B. abortus RB51 vaccine by using indirect ELISA Materials and Methods: Each of the three randomly selected B. abortus sero-negative native cows and three buffaloes received 2.0 ml imported commercial B. abortus SRB51 vaccine subcutaneously in the neck region at day 0 and then booster dose at 60 days after first vaccination with similar dose and route. Each of the collected serum samples of both the cattle and buffaloes was tested to detect the antibody status by using commercial indirect ELISA kit. Results: The results showed that the OD value of the serum of cows and buffalos before inoculation of RB51 B. abortus vaccine was 0.088 ± 0.009 and 0.096  0.011 at 0 week and 0.124 ± 0.018 and 0.111  0.010 at 1st week, near about the negative control OD value (0.106). After that, the OD value started to rise from the 2nd week (OD value (0.144 ± 0.023 and 0.1333  0.007) and reached to a peak level at 90 days (OD value 0.376  0.0080 and 0.316  0.219) and then started to decline from 120 days (OD value 0.2963  0.0416 and 0.2863  0.070) to 180 days (OD value 0.1943 0.073 and 0.176 0.172) in cows and buffalos respectively. Conclusion: This study suggests that the RB51 vaccination has induced satisfactory HIR with initial inoculation but significantly higher immune responses with booster immunization which enhancing immunity against both in the cattle and buffaloes. The CMI plays major role in protection against brucellosis needs further investigation in both cattle and buffaloes in Bangladesh. Keywords: Brucellosis, SRB51 vaccine, Humoral immune response (HIR), I-ELISA, Cattle and Buffaloes

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Comparison of Humoral Immune Responses Between Cattle and Buffaloes Immunized With Commercial Brucella Abortus Strain Rb51 Vaccine in Bangladesh

Mohamud¹,

Mohamud²,

Rahman³

et al. 2020

JVMOHR

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“…A vacina B19, quando aplicada em animais com idade superior a oito meses, induz o surgimento de títulos de anticorpos que podem dificultar o diagnóstico (Poester et al 2000, Mathias et al 2001), e que, quando presentes, são detectados pelas técnicas de diagnóstico convencionais, pelo ELISA ID e pela FC, que não conseguem diferenciar anticorpos vacinais dos induzidos por infecção de campo (Viana et al 1982, Costa et al 1999, Ferraz 1999, Megid et al 1999, Samartino et al 1999. Os anticorpos vacinais não são detectados em animais adultos quando a vacinação com dose padrão respeita a idade preconizada pelo PNCEBT, pois, nestes casos, a titulação se estende, normalmente, até os 18 meses de idade (Costa et al 1999).…”

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Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus

Jardim¹,

Pires²,

Mathias³

et al. 2006

Pesq. Vet. Bras.

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Com o presente trabalho avaliou-se o uso de dose reduzida da vacina produzida com a amostra 19 de Brucella abortus, em rebanho adulto negativo para a enfermidade, por meio de técnicas de diagnóstico sorológico preconizadas pelo Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose Animal e por um ensaio indireto de imunoadsorção enzimática (ELISA ID). A prova de fixação de complemento detectou 46,77% de positivos, o antígeno acidificado tamponado 67,74%, o 2-mercaptoetanol com soroaglutinação lenta 87,09% e o ELISA ID 100%. A dose reduzida interferiu no diagnóstico sorológico. Nenhuma das técnicas apresentou especificidade adequada para uso em rebanho nestas condições, até 3 meses após a vacinação.

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“…The use of a reduced dose rate decreases the presence of vaccine-associated undesirable events, such as postvaccine reactors to conventional tests, abortion, and milk shedding [16]. The vaccination is recommended prior to the first gestation between 3 and 7 months of age to avoid abortion in pregnant animals [17]. When used at a reduced dose, Rev.…”

Section: Introductionmentioning

confidence: 99%

Comparative Field Trial Effect of Brucella spp. Vaccines on Seroconversion in Goats and Their Possible Implications to Control Programs

Molina-Sánchez¹,

Martínez-Herrera²,

Pardío-Sedas³

et al. 2020

New Insight Into Brucella Infection and Foodborne Diseases

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The aim of this study was to determine the seroprevalence of Brucella spp. in a goat flock and the seroconversion of three groups of animals vaccinated with Rev-1 (Brucella melitensis), RB51, and RB51-SOD (Brucella abortus) to estimate the level of protection conferred on susceptible females. Seventy-two animals were used by group. Goats were older than 3 months, seronegative to brucellosis, not vaccinated previously, and kept within positive flocks. Vaccinated animals received 2 mL of product subcutaneously in the neck region. The first block was injected with Rev-1; the second received RB51, and the third group was injected with RB51-SOD. Follow-up sampling was performed at 30, 60, 90, and 365 days postvaccination. The general prevalence of brucellosis for the three groups was 1.2% (95%CI:0.5-2.7). The seroconversion rate by day 30 after vaccination was 77.7% (95%CI:61.9-88.2) for goats vaccinated with Rev-1. At 365 days post vaccination, the percentage of seropositive goats declined to 13.8% (95%CI:6.0-28.6). At day 365 after vaccination, 2.7% (95%CI:0.4-14.1) and 5.5% (95%CI:1.5-18.1) of animals vaccinated with RB51 and RB51-SOD, respectively, became positive. Results show that the seroconversion induced by Brucella abortus RB51 and RB51-SOD vaccines is lower than that by Brucella melitensis Rev-1.

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The serological response of adult cattle after vaccination with Brucella abortus strain 19 and RB51

Cited by 11 publications

References 5 publications

Comparison of Humoral Immune Responses Between Cattle and Buffaloes Immunized With Commercial Brucella Abortus Strain Rb51 Vaccine in Bangladesh

Comparison of Humoral Immune Responses Between Cattle and Buffaloes Immunized With Commercial Brucella Abortus Strain Rb51 Vaccine in Bangladesh

Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus

Comparative Field Trial Effect of Brucella spp. Vaccines on Seroconversion in Goats and Their Possible Implications to Control Programs

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