The serosal mesothelium is a major source of smooth muscle cells of the gut vasculature

Wilm, Bettina; Ipenberg, Annemieke; Hastie, Nicholas D.; Burch, John; Bader, David M.

doi:10.1242/dev.02141

Cited by 292 publications

(321 citation statements)

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“…In the mouse, the development of the pulmonary vasculature matches lung branching morphogenesis from early E10.5 onwards and forms a dense capillary network encompassing the distal epithelium (16,17). To establish a temporal link between the development of the mesothelium and the pulmonary vasculature, we examined the expression of Wt1, a marker for lung mesothelial cells (5,7,18), at selected stages of lung development ( Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

“…Recent cell lineage labeling studies on the developing heart provide evidence that the surface epicardial mesothelium undergoes epithelial-mesenchymal transition (EMT) and migrates into the myocardium where it differentiates into various cell types, including endothelium, smooth muscle cells, and cardiomyocytes (5,8,10,11). In addition, lineage tracing and other studies show that the serosal mesothelium of the gut also contributes the majority of vascular smooth muscle cells (7,12).…”

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confidence: 99%

“…Whereas there is strong evidence in favor of the mesothelium having an important signaling role in the embryonic lung, whether it contributes to the formation of the pulmonary vascular system is unknown. Here, we take advantage of the Wt1-Cre transgenic mouse line utilized by Wilm et al (7) to lineage label the mesothelial cells during development. We demonstrate that lineage-labeled cells appear in the lung and give rise to vascular smooth muscle cells that populate the walls of vessels in both the proximal and distal lung.…”

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confidence: 99%

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Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development

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Wilm

Hasegawa

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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During mouse development, the sophisticated vascular network of the lung is established from embryonic day (E)Ϸ10.5 and continues to develop postnatally. This network is composed of endothelial cells enclosed by vascular smooth muscle, pericytes, and other mesenchymal cells. Recent in vivo lineage labeling studies in the developing heart and intestine suggest that some of the vascular smooth muscle cells arise from the surface mesothelium. In the developing lung, the Wilm's tumor 1 gene (Wt1) is expressed only in the mesothelial cells. Therefore, we lineage-labeled the mesothelium in vivo by using a Wt1-Cre transgene in combination with either Rosa26R lacZ , Rosa26R CAG-hPLAP , or Rosa26R EYFP reporter alleles. In all three cases, cells derived from lineage-labeled mesothelium are found inside the lung and as smooth muscle actin (SMA) and PDGF receptor-beta positive cells in the walls of pulmonary blood vessels. To corroborate this finding, we used 5-(and-6)-carboxy-2 ,7 -dichlorofluorescein diacetate, succinimidyl ester ''mixed isomers'' (CCFSE) dye to label mesothelial cells on the surface of the embryonic lung. Over the course of 72-h culture, dye-labeled cells also appear within the lung mesenchyme. Together, our data provide evidence that mesothelial cells serve as a source of vascular smooth muscle cells in the developing lung and suggest that a conserved mechanism applies to the development of blood vessels in all coelomic organs.lineage tracing ͉ blood vessel ͉ embryo ͉ pleura

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development

Que

Wilm

Hasegawa

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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233

187

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“…In the heart and gut, lineage-tracing of the outer mesothelial layer, using mesothelial-specific Wt1-Cre mouse strains, has shown that the mesothelium is the source of $80% of the vascular smooth muscle cells during embryonic development (Wilm et al, 2005;Zhou et al, 2008). Similar A: Hematoxylin and eosin stained section of an embryonic day (E) 12.5 left lung lobe.…”

Section: Vascular Smooth Muscle Progenitorsmentioning

confidence: 99%

The building blocks of mammalian lung development

Rawlins

2010

Developmental Dynamics

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Progress has recently been made in identifying progenitor cell populations in the embryonic lung. Some progenitor cell types have been definitively identified by lineage-tracing studies. However, others are not as well characterized and their existence is inferred on the basis of lung morphology, or mutant phenotypes. Here, I focus on lung development after the specification of the initial lung primordium. The evidence for various lung embryonic progenitor cell types is discussed and future experiments are suggested. The regulation of progenitor proliferation in the embryonic lung, and its coordinate control with morphogenesis, is also discussed. In addition, the relationship between embryonic and adult lung progenitors is considered. Developmental Dynamics 240:463-476,

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“…The mainstream view of coronary artery formation from PE-derived ECs has been re-evaluated over the past decade through the use of Cre-LoxP genetic lineage-tracing approaches in mice [4][5][6][7]. Several different mouse Cre lines that label populations within the PE were developed.…”

mentioning

confidence: 99%

The mysterious origins of coronary vessels

Zhang

2013

Cell Res

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The serosal mesothelium is a major source of smooth muscle cells of the gut vasculature

Abstract: SummaryThe serosal mesothelium is a major source of smooth muscle cells of the gut vasculature

Cited by 292 publications

References 59 publications

Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development

Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development

The building blocks of mammalian lung development

The mysterious origins of coronary vessels

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