The severity of human peri‐implantitis lesions correlates with the level of submucosal microbial dysbiosis

Kröger, Annika; Hülsmann, Claudia; Fickl, Stefan; Spinell, Thomas; Hüttig, Fabian; Kaufmann, Frederic; Heimbach, André; Hoffmann, Per; Enkling, Norbert; Renvert, Stefan; Schwarz, Frank; Demmer, Ryan T.; Papapanou, Panos N.; Jepsen, Søren; Kebschull, Moritz

doi:10.1111/jcpe.13023

Cited by 72 publications

(78 citation statements)

References 62 publications

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“…The bacterial occurrence network analysis shown here (Figures and ) revealed a conserved pattern of dysbiosis within periodontitis and peri‐implantitis niches that commonly involved higher abundances of and/or positive co‐correlations between Prevotella spp . , Treponema , Porphyromonas, Tannerella, Filifactor, Parvimonas, Desulfobulbus, and Synergistetes taxa, consistent with findings from other microbiome studies (Apatzidou et al, ; Chen et al, ; Kröger et al, ; Lafaurie et al, ; Park et al, ; Schincaglia et al, ; Shi et al, ; Shiba et al, ). Several other taxa appear to play important etiological roles, including the as‐yet‐uncultivated Peptostreptococcaceae [XI] and Bacteroidetes [G‐5] phylotypes.…”

Section: Discussionsupporting

confidence: 86%

“…in both healthy and diseased subgingival plaque samples (Chen et al, ), as well as with Tannerella and Synergistetes taxa in periodontitis sites (Shi et al, ). Treponema parvum , which was originally isolated from periodontitis and necrotizing ulcerative gingivitis lesions (Wyss et al, ), was associated with BOP here and was recently associated with maximum pocket depth in peri‐implantitis sites (Kröger et al, ).…”

Section: Discussionmentioning

confidence: 96%

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Intra‐oral single‐site comparisons of periodontal and peri‐implant microbiota in health and disease

Chan

Liu

et al. 2019

Clinical Oral Implants Res

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Objective: Periodontitis and peri-implantitis are oral infectious-inflammatory diseases that share similarities in their pathology and etiology. Our objective was to characterize the single-site subgingival and submucosal microbiomes of implant-rehabilitated, partially dentate Chinese subjects (n = 18) presenting with both periodontitis and peri-implantitis. Materials and methods:Subgingival/submucosal plaque samples were collected from four clinically distinct sites in each subject: peri-implantitis submucosa (DI), periodontal pocket (DT), clinically healthy (unaffected) peri-implant submucosa (HI), and clinically healthy (unaffected) subgingival sulcus (HT). The bacterial microbiota present was analyzed using Illumina MiSeq sequencing.Results: Twenty-six phyla and 5,726 operational taxonomic units (OTUs, 97% sequence similarity cutoff) were identified. Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, Actinobacteria, Synergistetes, TM7, and Spirochaetes comprised 99.6% of the total reads detected. Bacterial communities within the DI, DT, HI, and HT sites shared high levels of taxonomic similarity. Thirty-one "core species" were present in >90% sites, with Streptococcus infantis/mitis/oralis (HMT-070/HMT-071/ HMT-638/HMT-677) and Fusobacterium sp. HMT-203/HMT-698 being particularly prevalent and abundant. Beta-diversity analyses (PERMANOVA test, weighted UniFrac) revealed the largest variance in the microbiota was at the subject level (46%), followed by periodontal health status (4%). Differing sets of OTUs were associated with periodontitis and peri-implantitis sites, respectively. This included putative "periodontopathogens," such as Prevotella, Porphyromonas, Tannerella, Bacteroidetes [G-5], and Treponema spp. Interaction network analysis identified several putative patterns underlying dysbiosis in periodontitis/peri-implantitis sites. Conclusions: Species (OTU) composition of the periodontal and peri-implant microbiota varied widely between subjects. The inter-subject variations in subgingival/ submucosal microbiome composition outweighed differences observed between implant vs. tooth sites, or between diseased vs. healthy (unaffected) peri-implant/ periodontal sites. | 761 YU et al. vincentii ("Fusobacterium Genus Probe 2"). Our data also correlate with that reported by Maruyama et al. (2014), who found that F. nucleatum, S. oralis, and Neisseria subflava, were abundant in both periodontitis and peri-implantitis lesions sampled from the same Japanese individuals. The respective compositions of the "core" microbiota reported in several other high-throughput TA B L E 3 Bacterial taxa present at higher abundances in the four respective clinical sites a Clinically healthy (unaffected) periodontal sites (HT) Periodontitis sites (DT) Halomonas sp. (OTU0) (core) Tannerella forsythia HMT-613 (OTU15) Escherichia coli HMT-574 (OTU17) Cardiobacterium valvalum HMT-540 (OTU33) Streptococcus mutans HMT-686 (OTU27) Capnocytophaga granulosa (OTU32) Veillonella dispar HMT-160 (OTU1252) Bacteroidales [G-2] sp. HMT-274...

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 96%

Intra‐oral single‐site comparisons of periodontal and peri‐implant microbiota in health and disease

Chan

Liu

et al. 2019

Clinical Oral Implants Res

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“…Microbial differences between healthy and diseased peri‐implant sites have been studied in detail (Al‐Ahmad et al, ; Kumar, Mason, Brooker, & O'Brien, ; Sanz‐Martin et al, ; Zheng et al, ). Increases in peri‐implant pocket depth have been shown to be associated with substantial changes in the submucosal microbiome and increasing levels of dysbiosis (Kröger et al, ).…”

Section: Peri‐implantitismentioning

confidence: 99%

“…, 2015). Increases in peri-implant pocket depth have been shown to be associated with substantial changes in the submucosal microbiome and increasing levels of dysbiosis (Kröger et al, 2018).…”

Section: Peri -Impl Ant Mucos Itismentioning

confidence: 99%

Peri‐implantitis and its prevention

Berglundh

Jepsen

Stadlinger

et al. 2019

Clinical Oral Implants Res

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117

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This perspective article on peri‐implantitis and its prevention was produced as a supplement to a 3‐D, computer‐animated film aiming at presenting key characteristics of peri‐implant health, the build‐up of a biofilm and the ensuing host‐response resulting in peri‐implant mucositis and, subsequently, peri‐implantitis. Treatment concepts for peri‐implantitis were briefly reviewed, and prevention of the condition was brought to attention as a priority in implant dentistry. The overview also highlighted the 2017 World Workshop on Classification of Periodontal and Peri‐implant diseases and Conditions, in which new disease definitions and case definitions were presented for peri‐implant health, peri‐implant mucositis, and peri‐implantitis.

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“…Without therapy, this may lead to loss of the tooth/implant [5,44]. In patients without a history of periodontitis, the implant had less bone loss and less inflammation in the long term [45]. In addition, periodontal pathogens are thought to be involved in the development of peri-implant inflammation [46].…”

Section: Discussionmentioning

confidence: 99%

Effect of Novel Micro-Arc Oxidation Implant Material on Preventing Peri-Implantitis

Huang

Zhou

et al. 2019

Coatings

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Dental implants occasionally fail for many reasons, especially peri-implantitis. The adhesion of bacteria to the surface of titanium is the initial factor in peri-implantitis. Therefore, the aim of this study was to assess the effect of a novel micro-arc oxidation (MAO) titanium on bacteria inhibition and regulation through periodontitis, and on a healthy saliva-derived biofilm, in vitro. MAO, sandblasting and acid etching (SLA), machined titanium and plasma-sprayed hydroxyapatite (HA) were selected for further study. The metabolic activity and biomass accumulation were tested using MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) and crystal violet assay after 24 h of anaerobic incubation. The structure was determined by scanning electron microscopy (SEM) and live/dead staining. Moreover, 16S rDNA sequencing was used to assess the microbial community. The results showed that biofilms on MAO were thinner compared to HA and SLA. In the periodontitis group, the biofilm accumulation and metabolic activity reached the highest levels in the HA group (p < 0.05); MAO titanium had the smallest biofilm accumulation and higher live/dead ratio; and the relative abundance of Lactobacillus in the SLA, HA and MAO groups increased significantly compared to the machined group (p < 0.05). In the healthy group, the relative abundance of Lactobacillus in the MAO group increased significantly compared to the other three groups (p < 0.05); the amount and metabolism activity of bacteria in the MAO group was lower (p < 0.05); MAO titanium had the least biofilm accumulation and a higher live/dead ratio. In conclusion, the novel MAO titanium had the ability to combat peri-implantitis by inhibiting the biofilm and regulating the microbial ecosystem to healthier conditions.

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The severity of human peri‐implantitis lesions correlates with the level of submucosal microbial dysbiosis

Cited by 72 publications

References 62 publications

Intra‐oral single‐site comparisons of periodontal and peri‐implant microbiota in health and disease

Intra‐oral single‐site comparisons of periodontal and peri‐implant microbiota in health and disease

Peri‐implantitis and its prevention

Effect of Novel Micro-Arc Oxidation Implant Material on Preventing Peri-Implantitis

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