The sexuality of Phlebiopsis gigantea

Korhonen, Kari T.; Kauppila, Pirkko

doi:10.29203/ka.1987.250

Cited by 20 publications

(15 citation statements)

References 13 publications

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“…Based on morphological characters, pairing experiments, and random amplified microsatellite (RAMS) fingerprinting, P. gigantea is regarded as a single species throughout Europe (E RIKSSON et al. 1981; K ORHONEN and K AUPPILA 1988; K ORHONEN et al. 1997; V AINIO et al.…”

Section: Introductionmentioning

confidence: 99%

Persistence of a biocontrol strain of Phlebiopsis gigantea in conifer stumps and its effects on within‐species genetic diversity

2001

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Fungal isolations and genetic fingerprinting were used to determine whether Phlebiopsis gigantea stump treatment against Heterobasidion annosum sl. using a single genotype (Rotstop) would affect the genetic diversity of P. gigantea populations. The survival time of P. gigantea was longer in Norway spruce (Picea abies) stumps compared to Scots pine (Pinus sylvestris) as no isolates were obtained from pine stumps 6 years after treatment, whereas in about half of the spruce stumps the fungus was still present. The usage of Rotstop did not seem to increase the occurrence of the fungus 5 years after the treatment in fresh (1‐year‐old) untreated stumps within the same forest stands. All the isolates from the 6‐year‐old treated spruce stumps were identical in genotype with the Rotstop‐strain, whereas all isolates from the fresh untreated spruce and pine stumps differed from it. Within the treated pine stand, the biocontrol usage seemed to have caused a slight reduction in genetic markers not related to Rotstop, but there were no statistically significant differences between the marker frequencies and the local natural population. Thus, Rotstop is not likely to cause any immediate threat to the genetic diversity of P. gigantea.

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Section: Introductionmentioning

confidence: 99%

Persistence of a biocontrol strain of Phlebiopsis gigantea in conifer stumps and its effects on within‐species genetic diversity

2001

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“…It is not known whether homokaryotic fruit bodies of P. gigantea occur in the nature; all of the approximately 100 natural fruit bodies of P. gigantea analysed in our laboratory in this and in an earlier study (Korhonen and Kauppila 1988) were heterokaryotic. However, because homokaryotic fruit bodies are small they may remain unnoticed.…”

Section: Discussionmentioning

confidence: 92%

“…Path. 35 (2005) Phlebiopsis gigantea is a heterothallic fungus with bipolar (monofactorial) mating system (Korhonen and Kauppila 1988). The detection of sexual compatibility in matings is a bit problematic because no clear-cut morphological differences between heterokaryotic and homokaryotic mycelia have been found.…”

Section: Introductionmentioning

confidence: 99%

Interfertility between North American and European strains of Phlebiopsis gigantea

2005

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Thirteen homokaryotic strains of Phlebiopsis gigantea from Canada, six strains from the US and 10 strains from Europe were paired in all possible combinations in order to determine the degree of interfertility between them. The diagnosis of interfertility was based on the production of heterokaryotic fruit bodies in the pairings. Among the resulting 406 pairings, 253 (62%) fruited. Within the strains originating from Canada, USA and Europe, 64, 80 and 64% of the pairings fruited, respectively. The fruiting frequency in pairings between the Canadian and US strains was 65%, between the Canadian and European strains 55%, and between the US and European strains 67%. True hybridization between the European and North American P. gigantea was shown by analysing the single-spore progeny using DNA fingerprinting. In spite of the relatively low interfertility in pairings within and between continents, no clear indication of the existence of intersterility groups was found. The low interfertility is probably due to the ageing of the pure cultures and to deficient fruiting ability of certain heterokaryons on agar medium. The results strongly suggest that although the North American and European strains of P. gigantea show some differentiation they can be regarded as belonging to the same biological species.

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“…It is known that the mating system of P. gigantea is bipolar outcrossing, and that different European populations of the species are interfertile (Korhonen and Kauppila 1987). This raises the question to what extent basidiospores from the introduced Rotstop † strain mate with individuals of resident P. gigantea populations and what impact this may have on intraspecific genetic diversity.…”

Section: Introductionmentioning

confidence: 99%

Impact of the biological control agentPhlebiopsis giganteaon its resident genetic structure in the Baltic Sea area

Samils

Vasaitis

Stenlid

2009

Biocontrol Science and Technology

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The basidiomycete Phlebiopsis gigantea (Fr.) Jü lich has been used in Swedish forestry as a biocontrol agent against the root and butt-rot pathogen Heterobasidion annosum s.l. on freshly cut Picea abies stumps since the early 1990s. Until 2005, the commercial preparation of this biological stump treatment, Rotstop † , has been based on a single strain of P. gigantea that has been applied on more than 47,000 ha annually in Fennoscandia. This paper reports on the spread of genetic material from the Rotstop † biocontrol strain of P. gigantea to resident populations of P. gigantea. We conclude that the inoculated fungus remained to a large extent restricted to the treated plots and did not spread to the adjacent areas, dominated by the natural spore deposition from resident populations of the fungus. Furthermore, the study demonstrates high genetic diversity and low geographic differentiation in P. gigantea populations in the geographical area around the Baltic Sea.

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The sexuality of Phlebiopsis gigantea

Cited by 20 publications

References 13 publications

Persistence of a biocontrol strain of Phlebiopsis gigantea in conifer stumps and its effects on within‐species genetic diversity

Persistence of a biocontrol strain of Phlebiopsis gigantea in conifer stumps and its effects on within‐species genetic diversity

Interfertility between North American and European strains of Phlebiopsis gigantea

Impact of the biological control agentPhlebiopsis giganteaon its resident genetic structure in the Baltic Sea area

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