The Significance and Applications of Bacteriophage in Bacteriological and Virus Research 1

Craigie, James

doi:10.1128/mmbr.10.3-4.73-88.1946

Cited by 15 publications

(4 citation statements)

References 24 publications

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“…Viruses of bacteria (bacteriophages, or phages) have been used for the diagnostics of bacterial infections since the 1930s [27] . Bacteriophage-based detection exploits fundamental properties of lytic phages: specific targeting, infection and lysis of host cells with simultaneous amplification of phage particles.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Detection of Yersinia pestis Using Amplification of Plague Diagnostic Bacteriophages Monitored by Real-Time PCR

Sergueev

Borschel

et al. 2010

PLoS ONE

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Background Yersinia pestis, the agent of plague, has caused many millions of human deaths and still poses a serious threat to global public health. Timely and reliable detection of such a dangerous pathogen is of critical importance. Lysis by specific bacteriophages remains an essential method of Y. pestis detection and plague diagnostics.Methodology/Principal FindingsThe objective of this work was to develop an alternative to conventional phage lysis tests – a rapid and highly sensitive method of indirect detection of live Y. pestis cells based on quantitative real-time PCR (qPCR) monitoring of amplification of reporter Y. pestis-specific bacteriophages. Plague diagnostic phages ϕA1122 and L-413C were shown to be highly effective diagnostic tools for the detection and identification of Y. pestis by using qPCR with primers specific for phage DNA. The template DNA extraction step that usually precedes qPCR was omitted. ϕA1122-specific qPCR enabled the detection of an initial bacterial concentration of 103 CFU/ml (equivalent to as few as one Y. pestis cell per 1-µl sample) in four hours. L-413C-mediated detection of Y. pestis was less sensitive (up to 100 bacteria per sample) but more specific, and thus we propose parallel qPCR for the two phages as a rapid and reliable method of Y. pestis identification. Importantly, ϕA1122 propagated in simulated clinical blood specimens containing EDTA and its titer rise was detected by both a standard plating test and qPCR.Conclusions/SignificanceThus, we developed a novel assay for detection and identification of Y. pestis using amplification of specific phages monitored by qPCR. The method is simple, rapid, highly sensitive, and specific and allows the detection of only live bacteria.

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Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Detection of Yersinia pestis Using Amplification of Plague Diagnostic Bacteriophages Monitored by Real-Time PCR

Sergueev

Borschel

et al. 2010

PLoS ONE

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“…In the 1940s Craigie observed that many strains of Salmonella enterica serotype Typhi carried temperate phages [1,2]. He believed that carriage of these phages made their host strains resistant to the Vi-typing phages.…”

Section: Introductionmentioning

confidence: 99%

Phage conversion inSalmonella entericaserotype Enteritidis: implications for epidemiology

Rankin

Platt

1995

Epidemiol. Infect.

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A model system for the study of phage conversion of Salmonella enterica serotype Enteritidis is reported. Temperate phages 1,2,3 and 6 from the phage typing scheme were used to convert several individually recognized phage types into others. Phage type 4 was converted to PT8, PT6a to PT4, PT6a to PT7, PT13 to PT13a and PT15 to PT11; some new phage lysis patterns were also detected. This model was used to examine the relationships between phage types within a previously defined clonal lineage, SECLIII, to establish whether or not Enteritidis like Salmonella enterica serotype Typhi and Salmonella enterica serotype Paratyphi B possessed type determining phages. We were able to convert PT1 to PT20, and PT15 to PT11.

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“…all other hosts. The adapted phages were supposed to be hostrange mutants (see Craigie, 1946). Recently, however, many of them were shown to be due to hostinduced modifications of Vi-phage II Felix, 1952, 1953a).…”

Section: The Causes Of the Host-induced Modificationsmentioning

confidence: 99%

Host-Induced Modifications of Viruses

Luria¹

1953

Cold Spring Harbor Symposia on Quantitative Biology

142

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The Significance and Applications of Bacteriophage in Bacteriological and Virus Research 1

Cited by 15 publications

References 24 publications

Rapid and Sensitive Detection of Yersinia pestis Using Amplification of Plague Diagnostic Bacteriophages Monitored by Real-Time PCR

Rapid and Sensitive Detection of Yersinia pestis Using Amplification of Plague Diagnostic Bacteriophages Monitored by Real-Time PCR

Phage conversion inSalmonella entericaserotype Enteritidis: implications for epidemiology

Host-Induced Modifications of Viruses

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