The significance of mannose-binding lectin gene polymorphisms on the risk of BK virus coinfection in women with human papillomavirus–positive cervical lesions

Comar, Manola; Segat, Ludovica; Crovella, Sérgio; Bovenzi, Massimo; Cortini, Enzo; Tognon, Mauro

doi:10.1016/j.humimm.2011.04.002

Cited by 10 publications

(2 citation statements)

References 28 publications

(31 reference statements)

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“…When comparing ddPCR and qPCR, HPV DNA loads, determined in CIN/cervical cancer cell lines resulting HPV-positive with both assays, were well correlated (R 2 = 0.9706). These qualitative/quantitative data are in agreement with previous studies on CIN lesions (Andersson et al, 2005;Comar et al, 2011b;Cerasuolo et al, 2017) and cervical cancer cell lines (Ostrowska et al, 2011;Diao et al, 2015), thereby underling the robustness of this ddPCR. HPV genotyping indicated that CINs were mainly positive for HPV16, followed by HPV18.…”

Section: Discussionsupporting

confidence: 91%

Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

et al. 2020

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Human papillomaviruses (HPVs) are small DNA tumor viruses that mainly infect mucosal epithelia of anogenital and upper respiratory tracts. There has been progressive demand for more analytical assays for HPV DNA quantification. A novel droplet digital PCR (ddPCR) method was developed to simultaneously detect and quantify HPV DNA from different HPV types. DdPCR was initially tested for assay sensitivity, accuracy, specificity as well as intra-and inter-run assay variation employing four recombinant plasmids containing HPV16, HPV18, HPV11, and HPV45 DNAs. The assay was extended to investigate/quantify HPV DNA in Cervical Intraepithelial Neoplasia (CIN, n = 45) specimens and human cell lines (n = 4). DdPCR and qPCR data from clinical samples were compared. The assay showed high accuracy, sensitivity and specificity, with low intra-/inter-run variations, in detecting/quantifying HPV16/18/11/45 DNAs. HPV DNA was detected in 51.1% (23/45) CIN DNA samples by ddPCR, whereas 40% (18/45) CIN tested HPV-positive by qPCR. Five CIN, tested positive by ddPCR, were found to be negative by qPCR. In CIN specimens, the mean HPV DNA loads determined by ddPCR were 3.81 copy/cell (range 0.002-51.02 copy/cell), whereas 8.04 copy/cell (range 0.003-78.73 copy/cell) by qPCR. DdPCR and qPCR concordantly detected HPV DNA in SiHa, CaSki and Hela cells, whereas HaCaT tested HPV-negative. The correlation between HPV DNA loads simultaneously detected by ddPCR/qPCR in CINs/cell lines was good (R 2 = 0.9706, p < 0.0001). Our data indicate that ddPCR is a valuable technique in quantifying HPV DNA load in CIN specimens and human cell lines, thereby improving clinical applications, such as patient management after primary diagnosis of HPV-related lesions with HPV-type specific assays.

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Section: Discussionsupporting

confidence: 91%

Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

et al. 2020

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“…There has also been interest to determine whether MBL2 polymorphisms can influence rates of viral infections related to cancer, including human papillomavirus (HPV) infection [16], [27], hepatitis C virus [28] and BK virus coinfection in HPV-positive cervical lesions [29]. These studies have reported mixed results.…”

Section: Discussionmentioning

confidence: 99%

Mannose-Binding Lectin 2 Gene and Risk of Adult Glioma

et al. 2013

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Background and AimsThe immune system is likely to play a key role in the etiology of gliomas. Genetic polymorphisms in the mannose-binding lectin gene, a key activator in the lectin complement pathway, have been associated with risk of several cancers.MethodsTo examine the role of the lectin complement pathway, we combined data from prospectively collected cohorts with available DNA specimens. Using a nested case-control design, we genotyped 85 single nucleotide polymorphisms (SNPs) in 9 genes in the lectin complement pathway and 3 additional SNPs in MBL2 were tested post hoc). Initial SNPs were selected using tagging SNPs for haplotypes; the second group of SNPs for MBL2 was selected based on functional SNPs related to phenotype. Associations were examined using logistic regression analysis. All statistical tests were two-sided. Nominal p-values are presented and are not corrected for multiple comparisons.ResultsA total of 143 glioma cases and 419 controls were available for this analysis. Statistically significant associations were observed for two SNPs in the mannose-binding lectin 2 (ML2) gene and risk of glioma (rs1982266 and rs1800450, test for trend p = 0.003 and p = 0.04, respectively, using the additive model). One of these SNPs, rs1800450, was associated with a 58% increase in glioma risk among those carrying one or two mutated alleles (odds ratio = 1.58, 95% confidence interval = 0.99–2.54), compared to those homozygous for the wild type allele.ConclusionsOverall, our findings suggest that MBL may play a role in the etiology of glioma. Future studies are needed to confirm these findings which may be due to chance, and if reproduced, to determine mechanisms that link glioma pathogenesis with the MBL complement pathway.

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Association of MBL2 exon1 polymorphisms with high-risk human papillomavirus infection and cervical cancers: a meta-analysis

Wang

Yang

et al. 2016

Arch Gynecol Obstet

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The significance of mannose-binding lectin gene polymorphisms on the risk of BK virus coinfection in women with human papillomavirus–positive cervical lesions

Cited by 10 publications

References 28 publications

Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

Mannose-Binding Lectin 2 Gene and Risk of Adult Glioma

Association of MBL2 exon1 polymorphisms with high-risk human papillomavirus infection and cervical cancers: a meta-analysis

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