The Significant Cardiomyogenic Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells In Vitro

Nishiyama, Nobuhiro; Miyoshi, Shunichiro; Hida, Naoko; Uyama, Taro; Okamoto, Kazuma; Ikegami, Yasuyuki; Miyado, Kenji; Segawa, Kaoru; Terai, Masanori; Sakamoto, Michiie; Ogawa, Satoshi; Umezawa, Akihiro

doi:10.1634/stemcells.2006-0662

Cited by 110 publications

(112 citation statements)

References 41 publications

(73 reference statements)

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“…Adult MSCs have been reported to differentiate into cardiomyocytes after treatment with 5-azacytidine 34 and soluble factors. 35 However, fully functional cardiomyocytes with striated cytoskeleton and proper electrical coupling have not been observed. We also fail to observe fully functional cardiomyocytes, but ours is the first study to demonstrate that ECM components alone significantly affect the differentiation of stem cells, even those destined to become cells of the cardiovascular system.…”

Section: Santiago Et Almentioning

confidence: 99%

Heterogeneous Differentiation of Human Mesenchymal Stem Cells in Response to Extended Culture in Extracellular Matrices

Santiago

Pogemiller

Ogle

2009

Tissue Engineering Part A

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Extracellular matrix proteins (ECMs) guide differentiation of adult stem cells, but the temporal distribution of differentiation (i.e., heterogeneity) in a given population has not been investigated. We tested the effect of individual ECM proteins on lineage commitment of human bone marrow-derived mesenchymal stem cells (MSCs) over time. We exposed stem cell populations to ECM proteins representing the primary tissue structures of the body (i.e., collagens type I, III, IV; laminin; and fibronectin) and determined the lineage commitment of the stem cells at 1, 7, and 14 days. We found that collagens that can participate in the formation of fibrils guide differentiation of cardiomyocytes, adipocytes, and osteoblasts. ECMs of the basement membrane initiate differentiation of cardiomyocytes and osteoblasts but not adipocytes, and small facilitator ECMs (e.g., fibronectin) do not significantly affect stem cell differentiation. Differentiation was ECM-dependent because culture on tissue culture polystyrene, with consistent cell morphology, proliferation, and death, initiated differentiation of osteoblasts only. Thus, we show that ECMs independently trigger differentiation of human adult MSCs and that differentiation in this context can be guided down multiple lineages using the same ECM stimulus. This work highlights the importance of more clearly defining progenitor populations, especially those cultured in the presence of ECMs before transplantation.

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Section: Santiago Et Almentioning

confidence: 99%

Heterogeneous Differentiation of Human Mesenchymal Stem Cells in Response to Extended Culture in Extracellular Matrices

Santiago

Pogemiller

Ogle

2009

Tissue Engineering Part A

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“…When hUC-MSCs were treated with norm growth medium supplemented with acidic fibroblast growth factor (aFGF), sonic hedgehog (SHH), brain-derived growth factor (BDNF), nerve growth factor (NGF) and FBS and incubated at 37℃, 8% CO2, they were shown to differentiate into neurons. According to the research of Nishiyama N et al, it confirmed that hUC-MSCs can differentiate into cardiomyocyte cells using in vitro and in vivo models experiments [27] .…”

Section: Differentiation Capacities Of Huc-mscsmentioning

confidence: 95%

Unique Features and Clinical Application Prospect of Human Umbilical Cord Mesenchymal Stem Cells

Dilxat¹,

Li²,

Liu³

2016

International Journal of Orthopaedics

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MSCs. Moreover, MSCs harvested from hUC are pluripotent and immunologically naive. The morphology and biological characteristic of hUC-MSCs resembles that of MSCs isolated from bone marrow. CONCLUSION: Cell-based therapy is an emerging field, which can offer great therapeutic potential in the complex human diseases. Compared with bone marrow, hUC has been considered as an ideal source of MSCs due to their little ethical obstacles, painless procedures involved, lower risk of immune rejection and convenient sources for autologous cell therapy. The rapid development of umbilical cord blood banks appears to provide plentiful supply of stem cells for regenerative medicine and tissue engineering. INTRODUCTIONWith the increasing prevalence and incidence rates in the complex human diseases, e.g., bone regeneration disease, cardiovascular disease, diabetic disease and hematopoietic disease, there exits an urgent need to search for more effective and efficient therapies. Cellbased therapy is a novel and potential therapeutic approach, which can replace diseased or damaged cells with minimal side effects by introducing stem cells to these tissues or organs [1][2][3][4] . Currently, BMSCs have represented the golden type of stem cell for cell-based therapies. Experimental and clinical studies have showed the selfrenewal and multilineage differentiation capability of BMSCs [5][6][7] . However, it is quite difficult to produce large quantities of tissue/cells via bone marrow due to the decreasing frequency and differentiation potential with age. The harvesting of BMSCs also involves invasive procedures [8] . Therefore, the search for substitute of BMSCs is of ABSTRACT AIM: The purpose of the study was to summarize the unique features of human umbilical cord mesenchymal stem cells (hUC-MSCs) and their clinical application prospect.

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“…Isolation and Culture of MSCs-MSCs were isolated from UCs using the protocol developed by our group (4). Briefly, the tissue was washed with phosphate buffered saline and cut into small pieces (explants).…”

Section: Methodsmentioning

confidence: 99%

“…MSCs have proven to be a versatile source of cells capable of differentiating into various cellular lineages (1). MSCs have been isolated from a number of organs or tissues including adipose tissue (2), muscle (3), and umbilical cord (UC) (4). Our group has conducted proteome studies of chondrocytes with diverse pathologies (5,6) and has also achieved differentiation toward chondrocyte-like cells of MSCs from UC stroma using a new spheroid model and defined chondrogenic medium (7).…”

Section: Mesenchymal Stem Cells (Mscs)mentioning

confidence: 99%

Proteome Analysis During Chondrocyte Differentiation in a New Chondrogenesis Model Using Human Umbilical Cord Stroma Mesenchymal Stem Cells

Fuente

Mateos

Lesende-Rodríguez

et al. 2012

Molecular & Cellular Proteomics

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Umbilical cord stroma mesenchymal stem cells were differentiated toward chondrocyte-like cells using a new in vitro model that consists of the random formation of spheroids in a medium supplemented with fetal bovine serum on a nonadherent surface. The medium was changed after 2 days to one specific for the induction of chondrocyte differentiation. We assessed this model using reverse transcriptase-polymerase chain reaction, flow cytometry, immunohistochemistry, and secretome analyses. The purpose of this study was to determine which proteins were differentially expressed during chondrogenesis. Differential gel electrophoresis analysis was performed, followed by matrix-assisted laser desorption/ionization mass spectrometry protein identification. A total of 97 spots were modulated during the chondrogenesis process, 54 of these spots were identified as 39 different proteins and 15 were isoforms. Of the 39 different proteins identified 15 were down-regulated, 21 were up-regulated, and 3 were up-and down-regulated during the chondrogenesis process. Using Pathway Studio 7.0 software, our results showed that the major cell functions modulated during chondrogenesis were cellular differentiation, proliferation, and migration. Five proteins involved in cartilage extracellular matrix metabolism found during the differential gel electrophoresis study were confirmed using Western blot. The results indicate that our in vitro chondrogenesis model is an efficient and rapid technique for obtaining cells similar to chondrocytes that express proteins characteristic of the cartilage extracellular matrix. These chondrocyte-like cells could prove useful for future cell ther-

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The Significant Cardiomyogenic Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells In Vitro

Abstract: ABSTRACT

Cited by 110 publications

References 41 publications

Heterogeneous Differentiation of Human Mesenchymal Stem Cells in Response to Extended Culture in Extracellular Matrices

Heterogeneous Differentiation of Human Mesenchymal Stem Cells in Response to Extended Culture in Extracellular Matrices

Unique Features and Clinical Application Prospect of Human Umbilical Cord Mesenchymal Stem Cells

Proteome Analysis During Chondrocyte Differentiation in a New Chondrogenesis Model Using Human Umbilical Cord Stroma Mesenchymal Stem Cells

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