The simultaneous determination of 3,4-dihydroxyphenylethylene glycol, 3,4-dihydroxyphenylacetic acid and catecholamines in brain tissue by high performance liquid chromatography with electrochemical detection

Howes, L. G.; Summers, Roger J.; Rowe, P. R.; Loui, W.J.

doi:10.1016/0304-3940(83)90390-7

Cited by 36 publications

(19 citation statements)

References 17 publications

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“…The catecholamines were eluted with 250 ,ul 0.1 M perchloric acid, and aliquots were assayed by highperformance liquid chromatography with electrochemical detection. 17 The between-day coefficient of variation for the assay of each catecholamine was 8%, and the limit of detection was 20 pg. Urine catecholamines were extracted by a combined cation exchange column technique.18 Samples were assayed by high-performance liquid chromatography with electrochemical detection with DHBA as an internal standard.…”

Section: Materials and Methods Induction Of Heart Failurementioning

confidence: 98%

Neurohumoral responses to chronic myocardial infarction in rats.

et al. 1988

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In chronic cardiac failure, various neurohumoral mechanisms are activated to sustain blood volume, blood pressure, and organ perfusion. Using the coronary artery ligation model of heart failure in the rat, we have measured changes in vasoactive hormone secretion and related these changes to salt and water status during a 1-month period. When compared with controls, rats with infarction had a marked rise in plasma atrial natriuretic peptide (294 ± 59 vs. 79±10 pg/ml, p

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Section: Materials and Methods Induction Of Heart Failurementioning

confidence: 98%

Neurohumoral responses to chronic myocardial infarction in rats.

et al. 1988

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“…These levels are surprisingly high since the hypothalamic content of EPI is low and ranges from 17 to 40 pg/mg wet tissue [25][26][27]. The EPI content of the hypothalamus ranges from 340 to 800 pg, the mean weight of the hypothalamus being 20 mg.…”

Section: Discussionmentioning

confidence: 99%

Epinephrine in Rat Hypophysial Portal Blood Is Derived Mainly from the Adrenal Medulla

et al. 1990

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This study was performed to determine if epinephrine (EPI) present in hypophysial portal blood has a peripheral or a central origin. Pituitary stalk and femoral arterial EPI plasma levels were simultaneously measured in rats anesthetized with thiopental or urethane. EPI was measured by two different methods: radioenzymatic assay or HPLC followed by electrochemical detection. In thiopental-anesthetized rats, levels were higher in hypophysial portal than in femoral arterial plasma with both methods. In urethane-anesthetized rats, no difference in EPI concentrations was found between peripheral and portal plasma. A rapid blood volume depletion (15 ml/kg in 3 min) evoked a marked elevation of pituitary stalk and arterial EPI plasma concentration while norepinephrine (NE) and dopamine (DA) levels were unaffected. Under both types of anesthesia, the increase of EPI levels was higher in peripheral than in portal plasma. After removal of the adrenal glands, EPI was undetectable or barely detectable in pituitary stalk and arterial plasma. The hemorrhage-induced stimulation of EPI secretion was blunted in portal and peripheral plasma of adrenalectomized rats. These findings indicate that: (1) hypophysial portal EPI plasma concentration is highly dependent on an adrenal source; (2) hypothalamic EPI is not released into portal circulation in response to acute hemorrhage, and (3) anesthesia markedly influences catecholamines plasma levels.

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“…DHPG is a major metabolite of norepinephrine formed by oxidative deamination of norepinephrine within sympathetic neurones [9]. DHPG levels in heart [10] and brain [11] have been shown to be related to neuronal activity and to correlate with norepinephrine turnover [12]. Cardiac DHPG/NE ratios appear to provide a better index of elevated sympathetic activity in rats with chronic left ventricular failure than DHPG or norepinephrine levels alone, as they reflect the tendency for DHPG levels to rise and norepinephrine levels to fall.…”

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confidence: 99%

Comparative effects of angiotensin converting enzyme inhibition (perindopril) or diuretic therapy on cardiac hypertrophy and sympathetic activity following myocardial infarction in rats

Howes

Hodsman

et al. 1991

Cardiovasc Drug Ther

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The long-term effects of perindopril or chlorothiazide therapy were studied in rats after the induction of myocardial infarction by coronary artery ligation. Rats with infarction developed marked cardiomegaly, indicating the presence of chronic left ventricular dysfunction. The ratio of the norepinephrine metabolite, 3,4-dihydroxyphenylethylene glycol (DHPG) to norepinephrine (NE) was elevated in the right ventricle of rats with infarction, suggesting a chronic increase in cardiac sympathetic activity. Perindopril therapy commenced either immediately following infarction or 4 weeks following infarction reduced DHPG/NE ratios toward normal levels, and prevented or reversed cardiac hypertrophy. In contrast, chlorothiazide therapy significantly reduced DHPG/NE ratios but did not decrease cardiac hypertrophy. Perindopril reverses or prevents cardiac hypertrophy and chronic cardiac sympathetic hyperactivity following myocardial infarction, while chlorothiazide reduces cardiac sympathetic activity without influencing cardiomegaly.

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The simultaneous determination of 3,4-dihydroxyphenylethylene glycol, 3,4-dihydroxyphenylacetic acid and catecholamines in brain tissue by high performance liquid chromatography with electrochemical detection

Cited by 36 publications

References 17 publications

Neurohumoral responses to chronic myocardial infarction in rats.

Neurohumoral responses to chronic myocardial infarction in rats.

Epinephrine in Rat Hypophysial Portal Blood Is Derived Mainly from the Adrenal Medulla

Comparative effects of angiotensin converting enzyme inhibition (perindopril) or diuretic therapy on cardiac hypertrophy and sympathetic activity following myocardial infarction in rats

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