The simultaneous determination of vitamins A, E and β-carotene in bovine milk by high performance liquid chromatography–ion trap mass spectrometry (HPLC–MSn)

Effect of hemp seed oil supplementation on plasma lipid profile, liver function, milk fatty acid, cholesterol, and vitamin A concentrations in Carpathian goats

“…In particular, goat milk has high concentrations of caproic (6:0), caprylic (8:0), and capric (10:0) acids, known to exhibit antiobesity and antidiabetic properties (Nagao and Yanagita 2010). Butyric acid (4:0) and the major isomer of conjugated linoleic acid (CLA), cis-9,trans-11, have shown anticarcinogenic effects both in vitro and in vivo, and vitamin A may exert a major role in cell growth and differentiation (Mills et al 2011;Plozza et al 2012). By contrast, some saturated FA (SFA) (12:0, 14:0, 16:0), trans-18:1 FA, and cholesterol, if consumed in excess, have been associated with an increased cardiovascular disease risk (Shingfield et al 2008;Larsson et al 2012).…”

Quantification of selected fat soluble vitamins and carotenoids in infant formula and dietary supplements using fast liquid chromatography coupled with tandem mass spectrometry

“…For physiological samples such as human plasma, vitamins A, E, 25(OH) D 2 and 25(OH) D 3 were separated on a RP-C 18 column with gradient elution and all compounds were eluted in less than 15 min (Alvarez & De Mazancourt, 2001). Vitamins A, E and β-carotene in bovine milk were simultaneously separated on RP-C 18 column using methanol:water solvent system in about 30 min (Plozza, Trenerry, & Caridi, 2012) but the separation of vitamin D 3 was not included and reported as a separate method (Trenerry, Plozza, Caridi, & Murphy, 2011). Another LC/MS method was described for the determination of vitamin A, vitamin A acetate, vitamin A palmitate, vitamin E (α-tocopherol), vitamin E acetate, and coenzyme Q10 in multivitamin dietary supplements using a RP-C 30 stationary phase.…”

Determination of Antioxidant Compounds in Foodstuff

“…On the other hand, HPLC-MS/MS is mostly used for the quantification of compounds that are present in very low amounts in fi the sample, such as vitamins A and E or β-carotene [90,202]. Ultra performance liquid chromatography (UPLC) also exists, which presents higher sensitivity than HPLC; also, when coupled to ESI and/or MS it is described as being faster, consuming less amounts of eluent, presenting lower costs and taking less time for analysis.…”