The simultaneous downregulation of TRPM7 and MagT1 in human mesenchymal stem cells in vitro: Effects on growth and osteogenic differentiation

Castiglioni, Sara; Romeo, Valentina; Locatelli, Laura; Zocchi, Monica; Zecchini, Silvia; Maier, Jeanette A. M.

doi:10.1016/j.bbrc.2019.03.178

Cited by 14 publications

(17 citation statements)

References 26 publications

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“…62,63 Otherwise, some studies have reported that Mg ions can stimulate the proliferation of BMSCs and promote BMSCs differentiation into osteoblasts through regulation of transient receptor potential melastatin 7 (TRPM7) channel activity and acceleration of notch intracellular domain translocation. [64][65][66] It is worth mentioning that we did not detect the serum magnesium ion concentration, which can cause cardiovascular, renal, liver disease, as well as metabolism disorder if present in higher concentrations. 67 However, not only all the experimental animals were healthy before being sacrificed, but also there was no obvious inflammatory cell infiltration in H&E staining of bone tissue, which proved that all the composite scaffold has good biocompatibility.…”

Section: Discussionmentioning

confidence: 81%

Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

Meng

et al. 2020

J Tissue Eng

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Artificial bioactive materials have received increasing attention worldwide in clinical orthopedics to repair bone defects that are caused by trauma, infections or tumors, especially dedicated to the multifunctional composite effect of materials. In this study, a weakly alkaline, biomimetic and osteogenic, three-dimensional composite scaffold (3DS) with hydroxyapatite (HAp) and nano magnesium oxide (MgO) embedded in fiber (F) of silkworm cocoon and silk fibroin (SF) is evaluated comprehensively for its bone repair potential in vivo and in vitro experiments, particularly focusing on the combined effect between HAp and MgO. Magnesium ions (Mg2+) has long been proven to promote bone tissue regeneration, and HAp is provided with osteoconductive properties. Interestingly, the weak alkaline microenvironment from MgO may also be crucial to promote Sprague-Dawley (SD) rat bone mesenchymal stem cells (BMSCs) proliferation, osteogenic differentiation and alkaline phosphatase (ALP) activities. This SF/F/HAp/nano MgO (SFFHM) 3DS with superior biocompatibility and biodegradability has better mechanical properties, BMSCs proliferation ability, osteogenic activity and differentiation potential compared with the scaffolds adding HAp or MgO alone or neither. Similarly, corresponding meaningful results are also demonstrated in a model of distal lateral femoral defect in SD rat. Therefore, we provide a promising 3D composite scaffold for promoting bone regeneration applications in bone tissue engineering.

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Section: Discussionmentioning

confidence: 81%

Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

Meng

et al. 2020

J Tissue Eng

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“…Human mesenchymal stem cell (hMSC) studies demonstrated that TRPM7 and MagT1 may be important for osteoblastogenesis due to the increase in their expression during differentiation into osteoblasts. Moreover, downregulation of TRPM7 and MagT1 leads to autophagy, which could lead to accelerating osteoblastic differentiation, leading the authors to describe TRPM7 and MagT1 as possible osteoblastogenesis regulators [ 76 , 77 ]. However, others also showed that higher Mg 2+ may have adverse effects on bone metabolism, maybe in part due to changes in the expression of TRPM7 and in the homeostasis of other important metal ions for bone tissue, in addition to inhibiting ALP activity in osteoblasts [ 34 ].…”

Section: The Role Of Mg 2+ In Hematopoiesismentioning

confidence: 99%

A Review of the Action of Magnesium on Several Processes Involved in the Modulation of Hematopoiesis

Lima

Fock

2020

IJMS

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Magnesium (Mg2+) is an essential mineral for the functioning and maintenance of the body. Disturbances in Mg2+ intracellular homeostasis result in cell-membrane modification, an increase in oxidative stress, alteration in the proliferation mechanism, differentiation, and apoptosis. Mg2+ deficiency often results in inflammation, with activation of inflammatory pathways and increased production of proinflammatory cytokines by immune cells. Immune cells and others that make up the blood system are from hematopoietic tissue in the bone marrow. The hematopoietic tissue is a tissue with high indices of renovation, and Mg2+ has a pivotal role in the cell replication process, as well as DNA and RNA synthesis. However, the impact of the intra- and extracellular disturbance of Mg2+ homeostasis on the hematopoietic tissue is little explored. This review deals specifically with the physiological requirements of Mg2+ on hematopoiesis, showing various studies related to the physiological requirements and the effects of deficiency or excess of this mineral on the hematopoiesis regulation, as well as on the specific process of erythropoiesis, granulopoiesis, lymphopoiesis, and thrombopoiesis. The literature selected includes studies in vitro, in animal models, and in humans, giving details about the impact that alterations of Mg2+ homeostasis can have on hematopoietic cells and hematopoietic tissue.

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“…It has a structural role in the apatite crystals, it modulates the differentiation of the precursors of osteoclasts and osteoblasts, it is essential in controlling the proliferation and the activity of osteoblasts [3,21]. TRPM7 has a fundamental role in skeletogenesis [22], is critical for the survival of MSC [23] and plays a role in their osteogenic differentiation [10,14]. Also the Mg 2+ transporter MagT1 contributes to the osteogenic differentiation of MSC [14,24], while no data are available at the moment about the expression and the role of MagT1 in mature osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

“…TRPM7 has a fundamental role in skeletogenesis [22], is critical for the survival of MSC [23] and plays a role in their osteogenic differentiation [10,14]. Also the Mg 2+ transporter MagT1 contributes to the osteogenic differentiation of MSC [14,24], while no data are available at the moment about the expression and the role of MagT1 in mature osteoblasts. In this paper we focused our attention on mature osteoblast-like SaOS-2 cells [15][16][17].…”

Section: Discussionmentioning

confidence: 99%

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TRPM7 and MagT1 regulate the proliferation of osteoblast-like SaOS-2 cells through different mechanisms

Zocchi¹,

Scrimieri²,

Locatelli³

et al. 2020

Magnesium Research

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A correct Magnesium (Mg 2+ ) intake is essential for bone health. In particular, Mg 2+ deficiency inhibits the proliferation of osteoblast-like SaOS-2 cells by increasing nitric oxide (NO) production through the upregulation of inducible NO synthase. At the moment, little is known about the expression and the role of TRPM7, a channel/enzyme involved in Mg 2+ uptake, and MagT1, a Mg 2+ selective transporter, in SaOS-2 cells. Here we demonstrate that TRPM7 expression is not modulated by different extracellular concentrations of Mg 2+ and its silencing exacerbates growth inhibition exerted by low Mg 2+ through the activation of inducible NO synthase and consequent accumulation of NO. Moreover, MagT1 is upregulated in SaOS-2 cultured in high Mg 2+ and its silencing inhibits the growth of SaOS-2 cultured in media containing physiological or high Mg 2+ , without any modulation of NO production. We propose that TRPM7 and MagT1 are both involved in regulating SaOS-2 proliferation through different mechanisms.

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The simultaneous downregulation of TRPM7 and MagT1 in human mesenchymal stem cells in vitro: Effects on growth and osteogenic differentiation

Cited by 14 publications

References 26 publications

Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

A Review of the Action of Magnesium on Several Processes Involved in the Modulation of Hematopoiesis

TRPM7 and MagT1 regulate the proliferation of osteoblast-like SaOS-2 cells through different mechanisms

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