The soil methane sink.

Dunfield, Peter F.

doi:10.1079/9781845931896.0152

Cited by 99 publications

(141 citation statements)

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“…Aerobic methylotrophic bacteria in soils have been recognized as drivers of methane fluxes in terrestrial ecosystem (Dunfield, 2007;Trotsenko and Murrell, 2008;Conrad, 2009;Kolb, 2009a). However, their role in methanol exchange with the atmosphere has widely been overlooked, even though the annual global emission rate (5 Tmol per year) of methanol is close to that of methane (that is, 10 Tmol per year; Jacob et al, 2005), and most methylotrophs utilize methanol (Lidstrom, 2006;Chistoserdova et al, 2009;Kolb, 2009a).…”

Section: Introductionmentioning

confidence: 99%

Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

et al. 2012

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The role of soil methylotrophs in methanol exchange with the atmosphere has been widely overlooked. Methanol can be derived from plant polymers and be consumed by soil microbial communities. In the current study, methanol-utilizing methylotrophs of 14 aerated soils were examined to resolve their comparative diversities and capacities to utilize ambient concentrations of methanol. Abundances of cultivable methylotrophs ranged from 10 6 -10 8 g soilDW. Methanol dissimilation was measured based on conversion of supplemented 14 C-methanol, and occurred at concentrations down to 0.002 lmol methanol g soilDW À 1. Tested soils exhibited specific affinities to methanol (a 0 s ¼ 0.01 d À 1 ) that were similar to those of other environments suggesting that methylotrophs with similar affinities were present. Two deep-branching alphaproteobacterial genotypes of mch responded to the addition of ambient concentrations of methanol (p0.6 lmol methanol g soilDW) in one of these soils. Methylotroph community structures were assessed by amplicon pyrosequencing of genes of mono carbon metabolism (mxaF, mch and fae). Alphaproteobacteria-affiliated genotypes were predominant in all investigated soils, and the occurrence of novel genotypes indicated a hitherto unveiled diversity of methylotrophs. Correlations between vegetation type, soil pH and methylotroph community structure suggested that plant-methylotroph interactions were determinative for soil methylotrophs.

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Section: Introductionmentioning

confidence: 99%

Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

et al. 2012

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“…It has been welldocumented, however, that temperature changes typically have little effect on overall methanotrophic activity in soils, with Q 10 values typically between 1-2 (Börjesson et al, 2004;Crill, et al, 1994;King and Adamsen, 1992;Roslev et al, 1997;Whalen et al, 1990). Such low values are attributed to slow mass transfer of methane (Dunfield, 2007), although occasionally higher Q 10 values are reported in soils that may have higher gas diffusivity (Christophersen, et al, 2000;MacDonald, et al, 1997) or exposed to methane concentrations greater than 10,000 ppmv (De Visscher, et al, 2001). At temperature extremes, however, i.e., <10 ºC and >40 ºC, methane oxidation is significantly limited in forest and landfill cover soil samples Christophersen, et al, 2000;Czepial, et al, 1996;Whalen and Reeburgh, 1996), likely due to inhibition of mesophilic methanotrophs.…”

Section: Temperaturementioning

confidence: 90%

“…Interestingly, in methanotrophic Verrucomicrobia, possibly a variation of the serine pathway was present supported by identifying some genes that encode enzymes part of the serine pathway (Dunfield, et al, 2007;Pol, et al, 2007). Additionally, in these studies, all the genes to form a complete Calvin-Benson cycle were identified (Dunfield, et al, 2007) or genes for Ribulose-1,5-bisphosphate carboxylase/oxylase (RuBisCO) was detected (Pol, et al, 2007). Unlike the RuMP and serine pathway where formaldehyde, a product of the oxidation of methanol shown in Figure 3, is utilized to produce biomass, the Calvin-Benson cycle utilizes CO 2 .…”

Section: Physiology and Phylogeny Of Methanotrophsmentioning

confidence: 99%

“…Traditionally, methanotrophs were classified into two general groups (Type I and II) based on several characteristics such as cell morphology, membrane arrangement, carbon assimilation pathway, and predominant phospholipid fatty acids (PLFA) (Semrau, et al, 2010). Recently, however, acidophilic bacteria belonging to the phylum Verrucomicrobia exhibiting CH 4 oxidation capabilities have been isolated (Dunfield, et al, 2007;Islam, et al, 2008;Pol, et al, 2007) while the traditional Type I and Type II methanotrophs belong to Proteobacteria. Some of the characteristics of Type I and II methanotrophs and methanotrophic Verrucomicrobia are summarized in Table 4.…”

Section: Physiology and Phylogeny Of Methanotrophsmentioning

confidence: 99%

“…Methylocella has a vesicular membrane system connected to the cytoplasmic membrane, found on the periphery of the cytoplasm (Dedysh, et al, 2000(Dedysh, et al, , 2004Semrau, et al, 2010), while Methylocapsa contain packed vesicular membranes aligned to only one side of the cell (Dedysh, et al, 2002). The methanotrophs belonging to the phylum Verrucomicrobia have yet another different membrane system, i.e., vesicular/tubular membranes or filled with polyhedral organelles ( (Dunfield, et al, 2007;Islam, et al, 2008;Pol, et al, 2007. The identity of the unique membrane system of methanotrophic Verrucomicrobia has yet to be elucidated.…”

Section: Physiology and Phylogeny Of Methanotrophsmentioning

confidence: 99%

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Strategies to Optimize Microbially-Mediated Mitigation of Greenhouse Gas Emissions from Landfill Cover Soils

Semrau¹,

Lee²,

Im³

et al. 2010

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The overall objective of this project, "Strategies to Optimize Microbially-Mediated Mitigation of Greenhouse Gas Emissions from Landfill Cover Soils" was to develop effective, efficient, and economic methodologies by which microbial production of nitrous oxide can be minimized while also maximizing microbial consumption of methane in landfill cover soils. A combination of laboratory and field site experiments found that the addition of nitrogen and phenylacetylene stimulated in situ methane oxidation while minimizing nitrous oxide production. Molecular analyses also indicated that methane-oxidizing bacteria may play a significant role in not only removing methane, but in nitrous oxide production as well, although the contribution of ammonia-oxidizing archaea to nitrous oxide production can not be excluded at this time. Future efforts to control both methane and nitrous oxide emissions from landfills as well as from other environments (e.g., agricultural soils) should consider these issues. Finally, a methanotrophic biofiltration system was designed and modeled for the promotion of methanotrophic activity in local methane "hotspots" such as landfills. Model results as well as economic analyses of these biofilters indicate that the use of methanotrophic biofilters for controlling methane emissions is technically feasible, and provided either the costs of biofilter construction and operation are reduced or the value of CO 2 credits is increased, can also be economically attractive. 3 TABLE OF CONTENTSABSTRACT 2 EXECUTIVE SUMMARY 6

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Structural and functional response of methane‐consuming microbial communities to different flooding regimes in riparian soils

Bodelier

Bär-Gilissen

Meima-Franke

et al. 2011

Ecology and Evolution

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Climate change will lead to more extreme precipitation and associated increase of flooding events of soils. This can turn these soils from a sink into a source of atmospheric methane. The latter will depend on the balance of microbial methane production and oxidation. In the present study, the structural and functional response of methane oxidizing microbial communities was investigated in a riparian flooding gradient. Four sites differing in flooding frequency were sampled and soil-physico-chemistry as well as methane oxidizing activities, numbers and community composition were assessed. Next to this, the active community members were determined by stable isotope probing of lipids. Methane consumption as well as population size distinctly increased with flooding frequency. All methane consumption parameters (activity, numbers, lipids) correlated with soil moisture, organic matter content, and conductivity. Methane oxidizing bacteria were present and activated quickly even in seldom flooded soils. However, the active species comprised only a few representatives belonging to the genera Methylobacter, Methylosarcina, and Methylocystis, the latter being active only in permanently or regularly flooded soils.This study demonstrates that soils exposed to irregular flooding harbor a very responsive methane oxidizing community that has the potential to mitigate methane produced in these soils. The number of active species is limited and dominated by one methane oxidizing lineage. Knowledge on the characteristics of these microbes is necessary to assess the effects of flooding of soils and subsequent methane cycling therein.

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The soil methane sink.

Cited by 99 publications

References 0 publications

Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

Strategies to Optimize Microbially-Mediated Mitigation of Greenhouse Gas Emissions from Landfill Cover Soils

Structural and functional response of methane‐consuming microbial communities to different flooding regimes in riparian soils

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