The solute transport profile of two Aza-guanine transporters from the Honey bee pathogen Paenibacillus larvae

Alexander, Candace R; Dingman, Douglas W.; Schultes, Neil P.; Mourad, George

doi:10.1093/femsle/fny018

Cited by 5 publications

(2 citation statements)

References 48 publications

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“…To assess the thermodynamic aspect of the uptake of tZ, tZR, iP, and iPR in the BY-2 cells, we performed a series of accumulation assays in suspensions treated with 50 µM protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP) in dimethyl sulfoxide (DMSO). CCCP uncouples electron transfer from oxidative phosphorylation (Cavari et al, 1967; Cunarro and Weiner, 1975; Heytler, 1963), thus inhibiting proton gradient-dependent transport processes (Alexander et al, 2018; Culos and Watanabe, 1983; Stoffer-Bittner et al, 2018). We performed the same assays using cells treated with the corresponding amount of DMSO alone (mock treatment) as control.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Model of Cell-to-Cell Cytokinin Transport Reveals A Specific Mode of Cytokinin Riboside Influx

Nedvěd,

Hudeček,

Klíma

et al. 2024

Preprint

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1AbstractRibosylated forms of plant hormones cytokinins (CKs) are the dominant CK species translocated at long distances. Their particular roles in plant physiology imply the existence of a yet uncharacterized CK riboside-specific membrane transport system. In this work, we report significant differences in the kinetics of the membrane transport of CK nucleobases and ribosides and the overall affinity of membrane-bound carriers towards the two CK forms. We show that CK ribosides can inhibit the uptake of CK nucleobases in tobacco Bright Yellow 2 cell suspensions but not vice versa, confirming the existence of a membrane transport system that strictly recognizes CK ribosides.We further characterize the membrane transport of CK nucleobases and ribosides mediated by AtENT3 (EQULIBRATIVE NUCLEOSIDE TRANSPORTER 3), showing its preference towardstrans-zeatin riboside (tZR) over isopentenyl adenosine (iPR). With the molecular docking and molecular dynamics, we assess the interactions among the side chain of tZR and AtENT3 residues Tyr61 and Asp129, which are conserved in all AtENTs but not in the ENTs from non-plant species. Lastly, we show thatatent3mutation affects shoot phenotype, demonstrating the impact of CK riboside membrane transport on shoot development.

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Section: Resultsmentioning

confidence: 99%

Comprehensive Model of Cell-to-Cell Cytokinin Transport Reveals A Specific Mode of Cytokinin Riboside Influx

Nedvěd,

Hudeček,

Klíma

et al. 2024

Preprint

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“…The broad solute recognition of PlUacP is reminiscent of some bacterial NCS2 COG2252 and some eukaryotic NCS2 transporters. Recently, it was shown that two AzgA‐like transporters of P. larvae , PlAzg1 and PlAzg2, belonging to COG2252, display a wide solute transport profile including adenine, guanine, xanthine, uracil, and cytosine . Eukaryotic NCS2 transporters have similarly broad transport profiles including A. nidulans AzgA transporting adenine, guanine, and hypoxanthine ; Arabidopsis thaliana Azg1 and Azg2 transporting adenine and guanine; Zea mays Azg2–Azg3 transporters transporting adenine, guanine, cytosine, hypoxanthine, and xanthine (Mourad and Schultes, pers.…”

Section: Resultsmentioning

confidence: 99%

The solute transport and binding profile of a novel nucleobase cation symporter 2 from the honeybee pathogen Paenibacillus larvae

et al. 2018

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Here, we report that a novel nucleobase cation symporter 2 encoded in the genome of the honeybee bacterial pathogen Paenibacillus larvae reveals high levels of amino acid sequence similarity to the Escherichia coli and Bacillus subtilis uric acid and xanthine transporters. This transporter is named P. larvae uric acid permease‐like protein (PlUacP). Even though PlUacP displays overall amino acid sequence similarities, has common secondary structures, and shares functional motifs and functionally important amino acids with E. coli xanthine and uric acid transporters, these commonalities are insufficient to assign transport function to PlUacP. The solute transport and binding profile of PlUacP was determined by radiolabeled uptake experiments via heterologous expression in nucleobase transporter‐deficient Saccharomyces cerevisiae strains. PlUacP transports the purines adenine and guanine and the pyrimidine uracil. Hypoxanthine, xanthine, and cytosine are not transported by PlUacP, but, along with uric acid, bind in a competitive manner. PlUacP has strong affinity for adenine K m 7.04 ± 0.18 μ m , and as with other bacterial and plant NCS 2 proteins, PlUacP function is inhibited by the proton disruptor carbonyl cyanide m‐chlorophenylhydrazone. The solute transport and binding profile identifies PlUacP as a novel nucleobase transporter.

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A nucleobase cation symporter 2, EaXanP, from Erwinia amylovora transports xanthine

et al. 2020

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The solute transport profile of two Aza-guanine transporters from the Honey bee pathogen Paenibacillus larvae

Cited by 5 publications

References 48 publications

Comprehensive Model of Cell-to-Cell Cytokinin Transport Reveals A Specific Mode of Cytokinin Riboside Influx

Comprehensive Model of Cell-to-Cell Cytokinin Transport Reveals A Specific Mode of Cytokinin Riboside Influx

The solute transport and binding profile of a novel nucleobase cation symporter 2 from the honeybee pathogen Paenibacillus larvae

A nucleobase cation symporter 2, EaXanP, from Erwinia amylovora transports xanthine

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