The Sources of Fibroblasts in Corneal Wound Repair

Weimar, Virginia

doi:10.1001/archopht.1958.00940080107014

Cited by 50 publications

(5 citation statements)

References 15 publications

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“…Furthermore, it has been estimated that most fibroblasts (65%) that contribute to the wound repair in the cornea are not derived from keratocytes but from circulating monocytes. 44 The overall extent of tissue damage corresponded well to the previously mentioned light microscopic findings.…”

Section: Commentsupporting

confidence: 86%

Nonmechanical Q-Switched Erbium:YAG Laser Trephination for Penetrating Keratoplasty

Stojković

Küchle²,

Seitz³

et al. 2003

Arch Ophthalmol

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To assess the alterations in human donor corneal tissue induced by Q-switched erbium (Er):YAG laser corneal trephination. Methods: Thirty human corneoscleral donor buttons unsuitable for transplantation were placed in an artificial chamber on an automated rotation device. Corneas were trephined with a Q-switched Er:YAG laser (wavelength, 2.94 µm; pulse duration, 400 nanoseconds) along (donor and recipient) aluminum silicate (ceramic) open masks. A spot diameter of 0.65 mm, energy setting of 50 mJ/pulse, and repetition rate of 5 Hz were used. Corneal thermal damage and cut regularity were quantitatively assessed in 24 corneas processed for light microscopy and by transmission and scanning electron microscopy. Results: The stromal thermal damage was the highest (mean [SD], 8.0 [2.7] µm) at a 150-µm cut depth and decreased downward. Cut regularity was very good and did not significantly differ between donors and recipients. Scanning electron microscopy confirmed that the cuts were highly regular; transmission electron microscopy revealed 2 distinctive subzones within the stromal thermal damage zone. Conclusions: Thermal damage induced by Q-switched Er:YAG nonmechanical corneal trephination was low, and the regularity of the cuts was very good. Clinical Relevance: The Q-switched Er:YAG laser may have the potential to become an alternative to the excimer laser for nonmechanical penetrating keratoplasty.

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Section: Commentsupporting

confidence: 86%

Nonmechanical Q-Switched Erbium:YAG Laser Trephination for Penetrating Keratoplasty

Stojković

Küchle²,

Seitz³

et al. 2003

Arch Ophthalmol

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“…He was able to describe migrating leukocytes as they passed through the walls of blood vessels in inflammatory exudates, and suggested that these leukocytes might become transformed to fibroblasts during the process of repair . Many investigations have been pursued since that time, several of which have tended to support Cohnheim's original suggestion (1,2,4,5,9,18,19,(21)(22)(23)31) . In contrast, numerous other studies have suggested the opposite, that is, that blood cells do not have the capacity to transform into connective tissue fiber-forming cells (3, 7, 8, 11-17, 20, 24-26) .…”

Section: Introductionmentioning

confidence: 99%

Wound Healing and Collagen Formation

Ross

Everett

Tyler

1970

The Journal of Cell Biology

217

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Healing skin wounds were studied in a series of parabiotic rats . The femurs of one parabiont of each pair were shielded whilst both animals were given 800 r from a Co60 source . The animals were wounded 3 days after irradiation . Each animal with partially shielded marrow was then given tritiated thymidine intraperitoneally daily while the cross-circulation was arrested by clamping . After the thymidine-3 H had cleared the blood, the clamp was released. Animals were sacrificed, and wounds were prepared for radioautography 1, 2, and 6 days after wounding. In the wounds of the shielded animals thymidine-3H was observed in epidermis, endothelium, leukocytes, fibroblasts, and mast cells . Only neutrophilic leukocytes, monocytes, and lymphocytes were labeled, as determined by light and electron microscope radioautography, in the wounds of each nonshielded parabiont . None of the many fibroblasts present were found to contain label in the wounds of the nonshielded parabionts through the 6 day period . These observations provide further evidence that wound fibroblasts do not arise from hematogenous precursors and, therefore, must arise from adjacent connective tissue cells .

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“…The cornea provides an accessible model system for studying the cooperative roles of various cell types and the mechanisms of their activation during wound healing. Within 1 hour after injury of rat corneas in vivo there is an activation or release of an apparent protease activity that appears to "trigger" the following: a) corneal invasion by polymomhonuclear leukocytes within 4-6 hours of injury (Weimar, 1957), b) activation of stromal cells to take up neutral red beginning 4 hours after injury (Weimar, 1958(Weimar, , 1959, and c) fibroblast formation at the wound edge in the stroma (Weimar, 1960). Inhibition of this protease activity blocks these events, but all can be restored in the wounded, protease-inhibited cornea by the topical application of trypsin (Weimar, 1960).…”

mentioning

confidence: 99%

Synergistic growth stimulation of corneal fibroblasts by components of mesodermal growth factor from murine submaxillary glands

Haraguchi

Knox

Weimar

et al. 1982

Journal Cellular Physiology

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Recycled mesodermal growth factor (R-MGF) is a pool of proteins of 26,000 molecular weight obtained by recycling gel chromatography of male murine submaxillary gland extracts. R-MGF strikingly accelerates corneal stromal wound healing in vivo, fibroblast growth and migration in cultured corneal buttons and is showing here to stimulate stromal fibroblast growth and division in tissue culture. Chromatographic fractionation of R-MGF has yielded several components, none of which has a greater biological potency than the parent R-MGF. In contrast, two components, MGF-I and -II, when recombined synergistically stimulate fibroblast response in tissue culture and organ culture in excess of those obtained with the parent R-MGF. Three MGF components (I, III, and IV) have been purified and are inactive at 10-15 micrograms/ml in organ culture but potently stimulate fibroblast responses when combined in pairs containing 7.5 micrograms of each component. The striking synergism in organ culture suggests that the stimulation of wound healing by R-MGF in vivo may also reflect synergistic action of more than one R-MGF component. Procedures for isolating gram quantities of R-MGF and for the purification of different R-MGF components by ion exchange chromatography are detailed.

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The Sources of Fibroblasts in Corneal Wound Repair

Cited by 50 publications

References 15 publications

Nonmechanical Q-Switched Erbium:YAG Laser Trephination for Penetrating Keratoplasty

Nonmechanical Q-Switched Erbium:YAG Laser Trephination for Penetrating Keratoplasty

Wound Healing and Collagen Formation

Synergistic growth stimulation of corneal fibroblasts by components of mesodermal growth factor from murine submaxillary glands

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