The “southeastern Europe” lineage of the brown dog tick Rhipicephalus sanguineus (sensu lato) identified as Rhipicephalus rutilus Koch, 1844: Comparison with holotype and generation of mitogenome reference from Israel

Šlapeta, Jan; Halliday, Bruce; Dunlop, Jason A.; Nachum‐Biala, Yaarit; Salant, Harold; Ghodrati, Sajjad; Modrý, David; Harrus, Shimon

doi:10.1016/j.crpvbd.2023.100118

Cited by 3 publications

(5 citation statements)

References 35 publications

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“…Seven ticks from Saudi Arabia morphologically fitted the general description of R. sanguineus ( s.l. ) with some exhibiting features congruent with R. linnaei as well as those of R. turanicus specimens ( Šlapeta et al, 2022 , 2023 ). Ticks underwent DNA isolation with DNA concentration ranging from 2.64 ng/μl to 0.40 ng/μl.…”

Section: Resultsmentioning

confidence: 87%

“…The four new mitogenome sequences (PP919883-PP919886) were appended to the mtDNA alignment of available R. sanguineus ( s.l .) ( Šlapeta et al, 2023 ). Both ML and ME reconstructed trees had identical topology for all species based on mitogenomes.…”

Section: Resultsmentioning

confidence: 99%

“…Generating mitogenomes of ticks and other parasites is possible using genome skimming via next-generation sequencing ( Jia et al, 2020 ; Šlapeta et al, 2021 ; Papaiakovou et al, 2023 ). The cost of genome skimming for ticks is comparable to the cost of PCR amplification and bi-directional Sanger sequencing of three or more amplicons, yet it generates all mitochondrial full coding sequences as well as both 12S and 16S rDNA gene sequences compared to a partial amplicon ( Kneubehl et al, 2022 ; Šlapeta et al, 2022 , 2023 ). Besides mitogenome, genome skimming enables screening for pathogens vectored by these ticks including bacteria, protozoans, fungi, nematodes, and viruses ( Jia et al, 2020 ; Paez-Triana et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

“…), and general acceptance of species (former lineages) identity determination based on partial mitochondrial sequences, there is currently no reference of a complete mitochondrial genome (mtDNA) also referred as ‘mitogenome’ from American specimen of R. linnaei . Mitogenomes enable comparison of the entire genome (∼14 kb) compared to small fragments (300–500 bp) and play a crucial role when comparing tick species and establishing robust taxonomy coupled with a morphological investigation ( Burger et al, 2014 ; Kneubehl et al, 2022 ; Šlapeta et al, 2022 , 2023 ; Barker et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

American mitogenome reference for the tropical brown dog tick, Rhipicephalus linnaei (Audouin, 1826)

Almazán,

Torres Rodríguez,

Alanazi

et al. 2024

Current Research in Parasitology & Vector-Borne Diseases

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Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

American mitogenome reference for the tropical brown dog tick, Rhipicephalus linnaei (Audouin, 1826)

Almazán,

Torres Rodríguez,

Alanazi

et al. 2024

Current Research in Parasitology & Vector-Borne Diseases

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“…Moreover, it cannot be excluded that other species of the Rhipicephalus genus can transmit B. vogeli ; for example, the tick from the “southeastern Europe” lineage of R. sanguineus s.l. found in Israel and Egypt has been identified as Rhipicephalus rutilus Koch, 1844, and was previously described by Koch in 1844 [ 210 ].…”

Section: Prevalence Of Babesia Vogelimentioning

confidence: 99%

Canine Babesiosis Caused by Large Babesia Species: Global Prevalence and Risk Factors—A Review

Zygner

Gójska-Zygner²,

Bartosik

et al. 2023

Animals

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Canine babesiosis is a disease caused by protozoan pathogens belonging to the genus Babesia. Four species of large Babesia cause canine babesiosis (B. canis, B. rossi, B. vogeli, and the informally named B. coco). Although canine babesiosis has a worldwide distribution, different species occur in specific regions: B. rossi in sub-Saharan Africa, B. canis in Europe and Asia, and B. coco in the Eastern Atlantic United States, while B. vogeli occurs in Africa, southern parts of Europe and Asia, northern Australia, southern regions of North America, and in South America. B. vogeli is the most prevalent large Babesia species globally. This results from its wide range of monotropic vector species, the mild or subclinical nature of infections, and likely the longest evolutionary association with dogs. The most important risk factors for infection by large Babesia spp. include living in rural areas, kennels or animal shelters, or regions endemic for the infection, the season of the year (which is associated with increased tick activity), infestation with ticks, and lack of treatment with acaricides.

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Molecular screening and genetic diversity of tick-borne pathogens associated with dogs and livestock ticks in Egypt

Senbill,

Karawia,

Zeb

et al. 2024

PLoS Negl Trop Dis

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Background The Middle East and North Africa (MENA) offer optimal climatic conditions for tick reproduction and dispersal. Research on tick-borne pathogens in this region is scarce. Despite recent advances in the characterization and taxonomic explanation of various tick-borne illnesses affecting animals in Egypt, no comprehensive examination of TBP (tick-borne pathogen) statuses has been performed. Therefore, the present study aims to detect the prevalence of pathogens harbored by ticks in Egypt. Methodology/Principal findings A four-year PCR-based study was conducted to detect a wide range of tick-borne pathogens (TBPs) harbored by three economically important tick species in Egypt. Approximately 86.7% (902/1,040) of the investigated Hyalomma dromedarii ticks from camels were found positive with Candidatus Anaplasma camelii (18.8%), Ehrlichia ruminantium (16.5%), Rickettsia africae (12.6%), Theileria annulata (11.9%), Mycoplasma arginini (9.9%), Borrelia burgdorferi (7.7%), Spiroplasma-like endosymbiont (4.0%), Hepatozoon canis (2.4%), Coxiella burnetii (1.6%) and Leishmania infantum (1.3%). Double co-infections were recorded in 3.0% (27/902) of Hy. dromedarii ticks, triple co-infections (simultaneous infection of the tick by three pathogen species) were found in 9.6% (87/902) of Hy. dromedarii ticks, whereas multiple co-infections (simultaneous infection of the tick by ≥ four pathogen species) comprised 12% (108/902). Out of 1,435 investigated Rhipicephalus rutilus ticks collected from dogs and sheep, 816 (56.9%) ticks harbored Babesia canis vogeli (17.1%), Rickettsia conorii (16.2%), Ehrlichia canis (15.4%), H. canis (13.6%), Bo. burgdorferi (9.7%), L. infantum (8.4%), C. burnetii (7.3%) and Trypanosoma evansi (6.6%) in dogs, and 242 (16.9%) ticks harbored Theileria lestoquardi (21.6%), Theileria ovis (20.0%) and Eh. ruminantium (0.3%) in sheep. Double, triple, and multiple co-infections represented 11% (90/816), 7.6% (62/816), and 10.3% (84/816), respectively in Rh. rutilus from dogs, whereas double and triple co-infections represented 30.2% (73/242) and 2.1% (5/242), respectively in Rh. rutilus from sheep. Approximately 92.5% (1,355/1,465) of Rhipicephalus annulatus ticks of cattle carried a burden of Anaplasma marginale (21.3%), Babesia bigemina (18.2%), Babesia bovis (14.0%), Borrelia theleri (12.8%), R. africae (12.4%), Th. annulata (8.7%), Bo. burgdorferi (2.7%), and Eh. ruminantium (2.5%). Double, triple, and multiple co-infections represented 1.8% (25/1,355), 11.5% (156/1,355), and 12.9% (175/1,355), respectively. The detected pathogens’ sequences had 98.76–100% similarity to the available database with genetic divergence ranged between 0.0001 to 0.0009% to closest sequences from other African, Asian, and European countries. Phylogenetic analysis revealed close similarities between the detected pathogens and other isolates mostly from African and Asian countries. Conclusions/Significance Continuous PCR-detection of pathogens transmitted by ticks is necessary to overcome the consequences of these infection to the hosts. More restrictions should be applied from the Egyptian authorities on animal importations to limit the emergence and re-emergence of tick-borne pathogens in the country. This is the first in-depth investigation of TBPs in Egypt.

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The “southeastern Europe” lineage of the brown dog tick Rhipicephalus sanguineus (sensu lato) identified as Rhipicephalus rutilus Koch, 1844: Comparison with holotype and generation of mitogenome reference from Israel

Cited by 3 publications

References 35 publications

American mitogenome reference for the tropical brown dog tick, Rhipicephalus linnaei (Audouin, 1826)

American mitogenome reference for the tropical brown dog tick, Rhipicephalus linnaei (Audouin, 1826)

Canine Babesiosis Caused by Large Babesia Species: Global Prevalence and Risk Factors—A Review

Molecular screening and genetic diversity of tick-borne pathogens associated with dogs and livestock ticks in Egypt

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