The soy-derived peptide Vglycin inhibits the growth of colon cancer cells <i>in vitro</i> and <i>in vivo</i>

Gao, Chang; Sun, Rui; Xie, Ya-Rong; An-li, Jiang; Li, Min; Chen, Zhengwang; Zhang, Ping; Jin, Honglin; Jiang, Feng

doi:10.1177/1535370217697383

Cited by 17 publications

(17 citation statements)

References 29 publications

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“…The treatment of undifferentiated Caco2/TC7 cells with FPH exhibited a decrease in their viability, especially with PFPH that was correlated with incubation time. These results are in line with works reporting an anti-proliferative property of peptides and hydrolysates from soy [14], corn [12,31], chickpeas [13], and rice [11] on different cell models. Vglycin, a peptide isolated from soy, inhibited the proliferation of three types of colon cancer cells [14].…”

Section: Discussionsupporting

confidence: 91%

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Protein Hydrolysates from Fenugreek (Trigonella foenum graecum) as Nutraceutical Molecules in Colon Cancer Treatment

et al. 2019

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The application of plant extracts for therapeutic purposes has been used in traditional medicine since the plants are a source of a great variety of chemical compounds that possess biological activity. Actually, the effect of these extracts on diseases such as cancer is being widely studied. Colorectal adenocarcinoma is one of the main causes of cancer related to death and the second most prevalent carcinoma in Western countries. The aim of this work is to study the possible effect of two fenugreek (Trigonella foenum graecum) protein hydrolysates on treatment and progression of colorectal cancer. Fenugreek proteins from seeds were hydrolysed by using two enzymes separately, which are named Purafect and Esperase, and were then tested on differentiated and undifferentiated human colonic adenocarcinoma Caco2/TC7 cells. Both hydrolysates did not affect the growth of differentiated cells, while they caused a decrease in undifferentiated cell proliferation by early apoptosis and cell cycle arrest in phase G1. This was triggered by a mitochondrial membrane permeabilization, cytochrome C release to cytoplasm, and caspase-3 activation. In addition, the hydrolysates of fenugreek proteins displayed antioxidant activity since they reduce the intracellular levels of ROS. These findings suggest that fenugreek protein hydrolysates could be used as nutraceutical molecules in colorectal cancer treatment.

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Section: Discussionsupporting

confidence: 91%

“…However, Xue et al [13] reported that a chickpea-derived peptide inhibits the proliferation of breast cancer cells by increasing the p53 expression. Yet, Gao et al [14] found that peptides derived from soy Vglycin activate the expression levels of pro-apoptotic proteins and caspase-3.…”

Section: Introductionmentioning

confidence: 99%

Protein Hydrolysates from Fenugreek (Trigonella foenum graecum) as Nutraceutical Molecules in Colon Cancer Treatment

et al. 2019

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“…* represents a significant difference between the untreated groups of both macrophage types (p < 0.05), and different letters in each group represent significantly different IL-10 production (p < 0.05). polypeptide isolated from pea seeds, took 24 h to cause a significant increase in apoptosis in the CT-26, SW480, and NCI-H716 colon cancer cell lines at 10 µM (Gao et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Melittin Induced G1 Cell Cycle Arrest and Apoptosis in Chago-K1 Human Bronchogenic Carcinoma Cells and Inhibited the Differentiation of THP-1 Cells into Tumour- Associated Macrophages

Tipgomut

Wongprommoon

Takeo

et al. 2018

Asian Pac J Cancer Prev

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Background: Bronchogenic carcinoma (lung cancer) is one of the leading causes of death. Although many compounds isolated from natural products have been used to treat it, drug resistance is a serious problem, and alternative anti-cancer drugs are required. Here, melittin from Apis mellifera venom was used, and its effects on bronchogenic carcinoma cell proliferation and tumour-associated macrophage differentiation were evaluated. Methods: The half maximal inhibitory concentration (IC50) of melittin was measured by MTT. Cell death was observed by annexin V and propidium iodide (PI) co-staining followed by flow cytometry. Cell cycle arrest was revealed by PI staining and flow cytometry. To investigate the tumour microenvironment, differentiation of circulating monocytes (THP-1) into tumour-associated macrophages (TAMs) was assayed by sandwich-ELISA and interleukin (IL)-10 levels were determined. Cell proliferation and migration was observed by flat plate colony formation. Secretion of vascular endothelial growth factor (VEGF) was detected by ELISA. The change in expression levels of CatS, Bcl-2, and MADD was measured by quantitative RT-PCR. Results: Melittin was significantly more cytotoxic (p < 0.01) to human bronchogenic carcinoma cells (ChaGo-K1) than to the control human lung fibroblasts (Wi-38) cells. At 2.5 µM, melittin caused ChaGo-K1 cells to undergo apoptosis and cell cycle arrest at the G1 phase. The IL-10 levels showed that melittin significantly inhibited the differentiation of THP-1 cells into TAMs (p < 0.05) and reduced the number of colonies formed in the treated ChaGo-K1 cells compared to the untreated cells. However, melittin did not affect angiogenesis in ChaGo-K1 cells. Unlike MADD, Bcl-2 was up-regulated significantly (p < 0.05) in melittin-treated ChaGo-K1 cells. Conclusion: Melittin can be used as an alternative agent for lung cancer treatment because of its cytotoxicity against ChaGo-K1 cells and the inhibition of differentiation of THP-1 cells into TAMs.

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“…Soy milk digestion extract (daizein, glycitein, genistein) might inhibit the proliferation of in vitro human prostate cancer cells by regulating the expression of ERβ, PSA, p21, Cyclin D1 and CDK4 in an estrogen receptor (ER)-dependent manner [ 49 ]. The soy-derived peptide Vglycin decreased the tumor volume by 38% in xenograft mice transplanted with colon cancer (CT-26) cells [ 50 ]. The mechanisms of these phenomena may be due to the down regulation of CDK2 and Cyclin D1, G1/S phase cell cycle arrest and the dysregulated expression of Bax, Bcl-2, and Mcl-1.…”

Section: Discussionmentioning

confidence: 99%

The effect of the type of dietary protein on the development of ovarian cancer

et al. 2018

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We evaluated whether different dietary protein qualities (isocaloric diets involving animal (casein) or plant protein (soy protein) could inhibit the ovarian cancer growth in mice and improve their prognosis and whether chemotherapy had different tumor reducing effects on these mice. In the mice of the 20% plant protein group, the ovarian cancer growth at 5 weeks after tumor implantation was clearly reduced in comparison to the mice in the 20% animal protein group (p< 0.001). The serum levels of insulin and IGF-1 levels were both lower in the mice of the 20% plant protein group than in the mice of the 20% animal protein group (p<0.001 and p<0.01, respectively). Immunohistochemistry revealed that the level of eukaryotic initiation factor 4E-binding protein 1 (p-4EBP1) activity―one of the major downstream effectors of the mTOR pathway ―of the plant protein group was significantly weaker than that of the animal protein group (p<0.001). The prognosis of the 20% plant protein group was better than that of the 20% animal protein group (log-rank test, p=0.0062). The ovarian cancer growth in the 20% plant protein plus cisplatin treatment group was not significantly reduced in comparison to the 20% animal protein plus cisplatin treatment group. Our findings suggest that a diet high in plant protein reduces the growth of human ovarian cancer cells in mice compared to a diet high in animal protein, ―possibly through the lack of activation of the IGF/Akt/mTOR pathway, and leads to a better prognosis with or without cisplatin treatment.

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The soy-derived peptide Vglycin inhibits the growth of colon cancer cells in vitro and in vivo

Cited by 17 publications

References 29 publications

Protein Hydrolysates from Fenugreek (Trigonella foenum graecum) as Nutraceutical Molecules in Colon Cancer Treatment

Protein Hydrolysates from Fenugreek (Trigonella foenum graecum) as Nutraceutical Molecules in Colon Cancer Treatment

Melittin Induced G1 Cell Cycle Arrest and Apoptosis in Chago-K1 Human Bronchogenic Carcinoma Cells and Inhibited the Differentiation of THP-1 Cells into Tumour- Associated Macrophages

The effect of the type of dietary protein on the development of ovarian cancer

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