The Specific Gravity of Bull Spermatozoa and Seminal Plasma

Lavon, U.; Volcani, R.; Amir, D.; Danon, D.

doi:10.1530/jrf.0.0110447

Cited by 18 publications

(16 citation statements)

References 3 publications

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“…The values obtained compare well with those obtained with the same method by Lavon et al (1966) on bull spermatozoa which had a range of 1-0376 to 1-0927 and those obtained (using other methods) with stallion (Yamane, 1920), rabbit (Beatty, 1964) and bull (Anderson, 1946) spermatozoa. The observed values on baboon sperma¬ tozoa were higher, as expected, than the reported values for whole semen of 1-028 in man, 1-011 in the dog and 1-035 in bulls (Mann, 1964).…”

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confidence: 83%

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Specific Gravity Measurements on Baboon Spermatozoa

Kraemer¹,

Cruz²

1970

Reproduction

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The specific gravity (sp. gr.) of baboon spermatozoa was determined using the method described by Danon & Marikovsky (1964) for the determination of the density distribution of red blood cells and used by Lavon, Volcani, Amir & Danon (1966) for sp. gr. determination of bull spermatozoa and seminal plasma. Baboon semen was collected by electro-ejaculation using a rectal probe (Kraemer & Vera Cruz, 1969) and the ejaculate was fractionated within 5 min of ejaculation into a liquid and a coagulum fraction. Only the liquid fraction of the ejaculate was used for sp. gr. determinations. A battery of phthalate ester separating fluids of increasing sp. gr. (1\m=.\040 to 1\m=.\111) at increments of 0\m=.\002to 0\m=.\003was prepared. A column (10 mm) of separating fluid was drawn into capillary tubes of the type used for micro-haematocrit determinations (1\m=.\1 to 1\m=.\2mm internal diameter). Semen (43 mm) was then introduced by capillarity leaving approximately 20 mm of the tube unfilled. The dry ends of the tubes were flame sealed. The tubes were centrifuged at 12,000 g for 12 min. The height of packed cells appearing above the level of the phthalate ester mixture was measured using a microscope fitted with a micrometer. The lack of any appreciable effect of the phthalate esters on the osmotic pressure of the seminal fluid is suggested by the fact that sperm cell motility is maintained during the specific gravity determinations and has been confirmed by direct measurements of osmotic pressure of seminal fluid before and after exposure to phthalate esters.The mean sp. gr. of the spermatozoa in one ejaculate or in a combined ejaculate (whenever the volume of one ejaculate was not sufficient) was calcu¬ lated according to the procedure of Lavon et al. (1966) : sp. gr. = d* ' xi+d2 ' 2.+d" ' x" 100 where d, to dn = % of spermatozoa present on top of the phthalate mixture in one capillary tube and not on top of the separating fluid in the preceding tube, the tubes being arranged in ascending order of sp. gr.x( to x" = average sp. gr. of two consecutive separating fluids. The sp. gr. (mean + S.E.) of six single ejaculates from six males was 1-0603 + 0-0101 with a range of 1-0500 to 1-0769, while that of the six combined ejacu¬ lates from twelve males was 1-0569 + 0-0070 ranging from 1-0452 to 1-0637.145

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supporting

confidence: 83%

“…gr.) of baboon spermatozoa was determined using the method described by Danon & Marikovsky (1964) for the determination of the density distribution of red blood cells and used by Lavon, Volcani, Amir & Danon (1966) for sp. gr.…”

mentioning

confidence: 99%

Specific Gravity Measurements on Baboon Spermatozoa

Kraemer¹,

Cruz²

1970

Reproduction

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“…Moreover, higher osmotic pressure of the epididymal secretion than that of blood (Lindhal & Kihlström, 1952;Glover;Guraya) is also responsible for dehydration of the sperm cells resulting decrease in the permeability of water content of the acrosome (Hamilton; Salisbury et al, 1978). Thus through progressive loss of water and a corresponding increase in the specific gravity (Lavon et al, 1966;Salisbury et al), swollen acrosomal membrane in caput gradually decreases in their shape and size during their transit (Hamilton; Jones et al; Hanks) resulting adherence of AC to the sperm nucleus (Jones, 1971(Jones, , 1975.…”

Section: Discussionmentioning

confidence: 99%

Structural Modification of the Black Bengal Buck (Capra hircus) Acrosome During Post-testicular Maturation of Spermatozoa

2014

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SUMMARY:The present study was conducted to measure various biometric parameters of intact/normal acrosomes (AC) collected respectively from caput, corpus and cauda epididymis and vas deferens of Black Bengal buck. Giemsa stained acrosomes were measured after camera lucida drawings. Observations revealed dimensional characters of the acrosomal cap diminished gradually and significantly (p<0.01, p<0.05) during spermatozoa maturation phases in the different regions of the excurrent duct. Shape and size of the AC were also found to be influenced significantly (p<0.01, p<0.05) by the age and body weight of the animals. The structural modification along with decrease in the morphology of the AC reflected one of the maturational indexes of the male gametes in Black Bengal buck.

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“…According to Stokes's formula, the sedimentation rate of the spermatozoa is = (d-d')xmxg, 3xdx S Sex ratio and sedimentation of bull semen 19 where d = density of spermatozoa. Opinions differ as to the average density of spermatozoa from 1-076 (Lavon, Volcani, Amir & Danon, 1966) to 1-335 (Lindhal & Kihlström, 1952). When the opinions about the real values of figures which are to be substituted in the formula differed from one another, we accepted those which overstated the final result.…”

Section: Discussionmentioning

confidence: 99%

Dependence of Primary and Secondary Sex Ratio on the Rapidity of Sedimentation of Bull Semen

Krzanowski¹

1970

Reproduction

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Bull semen was separated by sedimentation in milk, with egg-yolk and powdered milk added. The sex ratio (SR) of the progeny of 2866 cows inseminated with sedimented spermatozoa was compared to that of 2171 cows inseminated with unsedimented semen from the same sires. After insemination with the more rapidly sedimenting spermatozoa, a statistically significant preponderance of females was born compared to the number born after insemination with the remaining fractions of the sedimented semen. The percentage of males born after insemination with the slower sedimenting spermatozoa, however, was not higher than that in the control series. After insemination of sedimented semen, there was an increase in abortion and a deviation of the SR to the disadvantage of the numbers of males born, probably due to a more frequent abortion of male foetuses. The differentiation in the SR of the progeny, depending on the fraction used for insemination, remained, even allowing for correction as to the supposed sex of the aborted foetuses.

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The Specific Gravity of Bull Spermatozoa and Seminal Plasma

Cited by 18 publications

References 3 publications

Specific Gravity Measurements on Baboon Spermatozoa

Specific Gravity Measurements on Baboon Spermatozoa

Structural Modification of the Black Bengal Buck (Capra hircus) Acrosome During Post-testicular Maturation of Spermatozoa

Dependence of Primary and Secondary Sex Ratio on the Rapidity of Sedimentation of Bull Semen

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