The specific responses to mechanical wound in leaves and roots of Catharanthus roseus seedlings by metabolomics

Abstract

The mechanical wound is one of the unavoidable threats to survival of plants. More researchers focus on the effect of mechanical wound to the over-ground tissues. And the effects of wound to roots were frequently ignored, although it is an important organ for plant growth. In our studies, the metabolomics study was performed to reveal the mechanical wound effects in Catharanthus roseus on roots and leaves by combining gas chromatography-mass spectrometer (GC-MS), liquid chromatograph-mass spectrometer (LC-MS) and statistical analyses. The metabolic response of TIAs and PCs in plants to wound was most active at 0.5 h after treatment via Q value analysis. At this time point, then significantly responsive primary metabolites and specific secondary compounds (TIAs and PCs) were screened by PLS-DA score plot. In this case, the treatments of CK, LT (wound to leaves) and RT (wound to roots) were clearly distinguished. The targeted compounds include 8 sugars, 4 TIAs and 12 PCs and they displayed specific responses to CK, LT and RT treatments. Under RT group, plants invest more resources on the local responses using TIAs and the color reactions to regulate wound close using PCs. Whereas, LT group might lay emphasis on systemic responses via TIAs induced by SA (salicylic acid) and gallic acid. Our studies provide some basic data for further investigations of the defensive mechanism on roots treated by mechanical wound.

Metabolic Profiling of Primary Metabolites and Galantamine Biosynthesis in Wounded Lycoris radiata Callus

“…In the present study, we found that GAL accumulation was significantly increased with increasing exposure time. Some of the studies had reported that wounding stress significantly increases the contents of benzylisoquinoline alkaloids, shikimic acid, primary or secondary metabolites [ 31 , 45 , 46 ]. Specifically, integrated transcriptomic and metabolomic analysis of benzylisoquinoline alkaloids in wounded Papaver somniferum revealed significant increases in the narcotine and papaverine accumulation [ 45 ].…”

“…In carrot, wounding stress increased the production and accumulation of caffeoylquinic acids [ 29 , 30 ]. In a recent study, secondary metabolites in the mechanically wounded leaves and roots of Catharanthus roseus seedlings were analyzed [ 31 ]. However, metabolic profile of the C. roseus callus was not reported.…”

Looking for Hidden Enemies of Metabarcoding: Species Composition, Habitat and Management Can Strongly Influence DNA Extraction while Examining Grassland Communities

“…Additionally, terpenes and tannins and terpenoids are synthesized by the plant as a part of the defense system against herbivores [ 104 , 105 ]. The belowground part of the plant also induces the intensive production of secondary metabolites after wounding [ 106 ]. The whole plant synthesizes responsive secondary metabolites which can hinder the DNA extraction in the samples from intensively managed grasslands where the cutting and trampling was most severe.…”

The Power of Electropenetrography in Enhancing Our Understanding of Host Plant-Vector Interactions

“…Host plant metabolites play an important role in deterring or minimizing herbivory, and host metabolites have been shown to impact pest insect behavior and host preference in hemipteran pests [49,50,51]. Plant metabolites can be constitutive or induced [52], and can have diverse functions, including: Defense against herbivores or pathogens [53], as a response to mechanical damage [54,55], and as a response to environmental stress [56]. The leaf metabolites found in mandarin selections and pummelo provide additional data on the complex vector-host plant interactions of D. citri probing Citrus , but do not explain specific aspects of the observed probing differences.…”