THE SPECTROPHOTOMETRIC DETERMINATION OF CISPLATIN IN URINE, USING<i>o</i>-PHENYLENEDIAMINE AS DERIVATIZING AGENT

Anılanmert, Beril; Yalçın, Güler; Arıöz, Filiz; Dölen, Emre

doi:10.1081/al-100002709

Cited by 42 publications

(25 citation statements)

References 13 publications

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“…The DOX: pac site ratio was determined as described above, and the cisplatin: pac site ratio was measured according to the technique described by Anilanmert, et al . 39 .…”

Section: Methodsmentioning

confidence: 99%

Cell-Specific Delivery of Diverse Cargos by Bacteriophage MS2 Virus-like Particles

et al. 2011

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Virus-like particles (VLPs) of bacteriophage MS2 possess numerous features that make them well-suited for use in targeted delivery of therapeutic and imaging agents. MS2 VLPs can be rapidly produced in large quantities using in vivo or in vitro synthesis techniques. Their capsids can be modified in precise locations via genetic insertion or chemical conjugation, facilitating the multivalent display of targeting ligands. MS2 VLPs also self-assemble in the presence of nucleic acids to specifically encapsidate siRNA and RNA-modified cargos. Here we report the use of MS2 VLPs to selectively deliver nanoparticles, chemotherapeutic drugs, siRNA cocktails, and protein toxins to human hepatocellular carcinoma (HCC). MS2 VLPs modified with a peptide (SP94) that binds HCC exhibit a 104-fold higher avidity for HCC than for hepatocytes, endothelial cells, monocytes, or lymphocytes and can deliver high concentrations of encapsidated cargo to the cytosol of HCC cells. SP94-targeted VLPs loaded with doxorubicin, cisplatin, and 5-fluorouracil selectively kill the HCC cell line, Hep3B, at drug concentrations < 1 nM, while SP94-targeted VLPs that encapsidate a siRNA cocktail, which silences expression of cyclin family members, induce growth arrest and apoptosis of Hep3B at siRNA concentrations < 150 pM. Impressively, MS2 VLPs, when loaded with ricin toxin A-chain (RTA) and modified to co-display the SP94 targeting peptide and a histidine-rich fusogenic peptide (H5WYG) that promotes endosomal escape, kill nearly 100% of Hep3B cells (1 × 106 cells/mL population) at an RTA concentration of 100 fM without affecting the viability of control cells. Our results demonstrate that MS2 VLPs, due to their tolerance of multivalent peptide display and their ability to specifically encapsidate a variety of disparate cargos, induce selective cytotoxicity of cancer in vitro and represent a significant improvement in the characteristics of VLP-based delivery systems.

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“…The DOX: pac site ratio was determined as described above, and the cisplatin: pac site ratio was measured according to the technique described by Anilanmert, et al . 39 .…”

Section: Methodsmentioning

confidence: 99%

Cell-Specific Delivery of Diverse Cargos by Bacteriophage MS2 Virus-like Particles

et al. 2011

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“…In order to evaluate the drug in pharmaceutical dosage forms and in clinical situations and to optimize therapeutic regimens, analytical methods capable of separating the drug from its excipients or from its individual biotransformation products and detecting these species at therapeutically relevant levels are required [2]. Several efforts have been made so far that include derivative spectrophotometry [3][4][5][6], detection by phosphorescence of cisplatin in urine and plasma [7], atomic absorption spectrometry [8][9][10], electroanalytical methods [11], and highperformance liquid chromatography methods [12][13][14][15][16][17][18][19][20][21]. The structure of cisplatin hydrochloride is shown in Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Simple and Sensitive Spectrometric Method for Estimation of Cisplatin Hydrochloride in Tablet Dosage Forms: Application to Dissolution Studies

Basotra

Singh

Gulati

2013

ISRN Analytical Chemistry

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Cisplatin hydrochloride is an important chemotherapeutic drug for cancer treatment. It has a low molar absorptivity in the UV region and has no fluorescence. Therefore, a selective derivatizing reaction is required for its detection in bulk and pharmaceutical dosage form if detection by UV spectrophotometry is sought. In view of this, a simple, accurate, rapid, and cost-effective spectrophotometric method for its estimation has been developed by the complexation of the drug with ortho-phenylene diamine and monitoring the absorbance of formed green color at 706 nm. The method has been validated and successfully applied for the assay and dissolution studies of cisplatin hydrochloride tablets. The method demonstrated good linearity over the range from 0.4 to 1.4 μg/mL with a correlation coefficient of 0.9999. The accuracy of the method was 99.98%. The precision demonstrated relative standard deviation of less than 2.5%. The developed method was successfully applied for dissolution studies of sustained release tablets of cisplatin with a cumulative release of 86.7% in 12 hours. The proposed method can be applied in routine quality control in the pharmaceutical industries since it is precise, accurate, simple, and economic.

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“…Spectrophotometric kinetic methods based on the catalytic effect of the element upon the reactions in coloured (visible) or colourless (UV) solutions. It is known that aromatic amines are suitable for the investigation [23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

The investigation of thermodynamic parameters of kinetic reaction between o-phenylenediamine and gold (III)

Altun¹,

Akbaş²

2007

The Journal of Chemical Thermodynamics

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THE SPECTROPHOTOMETRIC DETERMINATION OF CISPLATIN IN URINE, USINGo-PHENYLENEDIAMINE AS DERIVATIZING AGENT

Cited by 42 publications

References 13 publications

Cell-Specific Delivery of Diverse Cargos by Bacteriophage MS2 Virus-like Particles

Cell-Specific Delivery of Diverse Cargos by Bacteriophage MS2 Virus-like Particles

Development and Validation of a Simple and Sensitive Spectrometric Method for Estimation of Cisplatin Hydrochloride in Tablet Dosage Forms: Application to Dissolution Studies

The investigation of thermodynamic parameters of kinetic reaction between o-phenylenediamine and gold (III)

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