Development and Validation of a Simple and Sensitive Spectrometric Method for Estimation of Cisplatin Hydrochloride in Tablet Dosage Forms: Application to Dissolution Studies

Abstract

Cisplatin hydrochloride is an important chemotherapeutic drug for cancer treatment. It has a low molar absorptivity in the UV region and has no fluorescence. Therefore, a selective derivatizing reaction is required for its detection in bulk and pharmaceutical dosage form if detection by UV spectrophotometry is sought. In view of this, a simple, accurate, rapid, and cost-effective spectrophotometric method for its estimation has been developed by the complexation of the drug with ortho-phenylene diamine and monitoring the absorbance of formed green color at 706 nm. The method has been validated and successfully applied for the assay and dissolution studies of cisplatin hydrochloride tablets. The method demonstrated good linearity over the range from 0.4 to 1.4 μg/mL with a correlation coefficient of 0.9999. The accuracy of the method was 99.98%. The precision demonstrated relative standard deviation of less than 2.5%. The developed method was successfully applied for dissolution studies of sustained release tablets of cisplatin with a cumulative release of 86.7% in 12 hours. The proposed method can be applied in routine quality control in the pharmaceutical industries since it is precise, accurate, simple, and economic.

Polydopamine Nanoparticles for Combined Chemo- and Photothermal Cancer Therapy

“…The morphology and size of the NPs did not show obvious change between PDA and PDA-PEG-CP NPs, as seen in Figure 2 b,c. The amount of cisplatin in the PEG-PDA NPs was measured to be 20% ( m / m ) using the OPDA method [ 31 ].…”

“…The total dialysis solutions were replaced with 10 mL fresh buffer at 6 h, 12 h, 24 h and 48 h, respectively. The amount of released cisplatin was measured via a modified literature procedure as follows [ 31 ]. Different aliquots (0.02 mL, 0.05 mL, 0.08 mL, 0.1 mL and 0.15 mL of 10 μg/mL, and 0.03 mL, 0.05 mL, 0.1 mL, 0.15 mL and 0.2 mL of 100 μg/mL) of cisplatin aqueous solution were diluted to 200 μL with water, followed by adding 200 μL of 0.7 mg/mL OPDA (freshly prepared in dimethylformamide (DMF)) and another 200 μL of phosphate buffer (0.1 M, pH 6.8).…”

Controlled Release Characteristics of Aqueous PEO‐PPO‐PEO Micelles With Added Malachite Green, Erythrosin, and Cisplatin Determined by UV–Visible Spectroscopy

“…shows a graphical example of the calibration curve of neat malachite green. In Table , we show a summary of our values of each ternary additives' wavelength ranges, concentration ranges (mg mL −1 ), slopes, y ‐intercepts, and correlation values ( R 2 ), and compare our results to the cisplatin complex by Basotra, Singh, and Gulati ().…”

“…For erythrosin, as the concentration increases, the solution progressed from light pink to a darker pink tint that absorbed in the 520–530 nm range. Basotra et al () mixed o ‐phenylenediamine with cisplatin to form a green colored complex with wavelength ranges 700–710 nm. For neat cisplatin, since the dissociation of the solid yellow‐colored cisplatin is a clear liquid, direct visual observation is not possible hence the importance of UV–Vis spectroscopy to measure absorbance in the UV (270–300 nm range).…”

Validation of a method to quantify platinum in cisplatin by inductively-coupled plasma

“…In the past, a spectrophotometric method was validated for the determination of cisplatin hydrochloride in the dissolution. Nonetheless, there is still little information regarding the determination of cisplatin in the aqueous media because most of the research has been focused upon the determination in biological fluids [19]. Cisplatin (total concentration of all species) can be estimated from the determination of Pt in the aqueous cisplatin samples.…”