The spectrum of chemosensitivity of two human pancreatic carcinoma xenografts

“…PK-9 was resistant to 5-Fu and MMC. Though it was reported that there was a great similarity in sensitivity to most of the drugs among pancreatic carcinoma xenografts with similar histology (Courtenay et al 1982), there was a marked difference in sensitivity to 5-Fu in our experiments using six pancreatic cancer cell lines. On the other hand, it was shown that in vitro chemosensitivity correlates with the prior chemotherapeutic treatment of patients, but not with cellular morphology (Carney et al 1983).…”

Section: Resultsmentioning

confidence: 66%

Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs.

Kobari

Hisano

Matsuno

et al. 1986

Tohoku J. Exp. Med.

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and -16, were established. They originated from either primary pancreatic cancer biopsy or liver metastasis biopsy, or xenografts of these biopsy specimens in athymic nude mice. The primary tumors were all well differentiated adenocarcinoma of pancreatic duct origin. The six established PK cell lines were all CEA positive and had tumorigenicity in athymic nude mice. Morphology of the xenografted tumors was closely similar to that of the original tumor. PK cells grew slowly with the doubling time of 41.3 to 82 hr and showed aneuploid chromosome pattern. High levels of glucose-6-phosphate dehydrogenase (G6PDH) and lactic dehydrogenase (LDH) were found in each cell extract. Trypsin was not detected in cell extracts except PK-8 and PK-9. In chemosensitivity test, all of PK cell lines were sensitive to aclacinomycin A (ACM), and PK-1 and PK-8 were sensitive to 5-Fluorouracil (5-Fu) at concentrations of 0.02 pg/ml, ACM and l;ug/ml, 5-Fu, when the drugs were used for over 48 hr. At higher concentrations, they showed time independent sensitivity to mitomycin C (MMC). PK-9 was resistant to 5-Fu and MMC. pancreatic cancer cell lines ; xenografted tumors ; chemosensitivity testIn spite of recent advances of diagnostic techniques, it is still difficult to diagnose pancreatic cancer in its early stage. Many chemotherapeutic and radiotherapeutic trials have been tested, but no good results have been obtained yet (Zimmerman et al.

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“…Interpatient differences may not be of interest to the cell biologist but they are of considerable potential importance to the therapist. A good example is in the work of Courtenay et al (1982) who found that clonogenic cells in two human pancreatic tumours had identical sensitivity to four cytotoxic drugs but showed a large difference in sensitivity to hexamethylmelamine. This sort of evidence lends support to efforts to test the sensitivity of human tumour cells to treatment before it is begun.…”

Section: Heterogeneity Of Clonogenic Tumour Cellsmentioning

confidence: 99%

Clonogenic tumour cells

Steel

1984

J. Cell. Physiol.

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The spectrum of chemosensitivity of two human pancreatic carcinoma xenografts

Cited by 10 publications

References 11 publications

The response to chemotherapy of a variety of human tumour xenografts

The response to chemotherapy of a variety of human tumour xenografts

Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs.

Clonogenic tumour cells

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