The sperm longevity and freezability in the modified BHSV extender of Thai Pradu-hangdum chicken

Abstract

Pradu-hangdum is a distinctive Thai native pure breed chicken. To conserve the pure breed of this chicken species, the artificial insemination was invested. The key to the highly successful output of this technique is high quality of semen. Hence, the objective of the present study is to characterize the Pradu-hangdam sperms and their longevity and freezability in the BHSV extender. The semen of the 30 randomized male chicken were collected. The macroscopic and microscopic examination were used to determine the sperm characters. The results revealed that the Pradu-hangdam sperms contained normal a spiral-shaped head, mid-piece and tail and normal white cream color. The mean number of sperm concentration was 5.24×109 ± 1.54 sperms/mL. The mean volume was 0.22 ± 0.08 mL. The results of longevity in mean total motility of sperm was 85.20%, 56.00% and 36.33% storage for 1, 24, and 48 hours after semen collection in extender, respectively. The longevity of sperm storage in extender decreased significantly at 24 and 48 hours (P<0.05). The freezability of Pradu-hangdam semen significant decreased from 81.45% to 57.02% of motility (P<0.05) but in the range of acceptable result for insemination. In conclusion, this study provides the basic knowledge of sperm characters and their longevity which decreases in relation to the time after collection even though it was preserved and frozen in the BHSV of the acceptable data. Furthermore, the freezing technique and fertility rate should be a further study in the long-term preservation of Pradu-hangdam sperms.

Semen characterization of Dang Surat Thai native chicken

“…The mean semen volume of the 20-month-old Dang Surat rooster was 265±23 µl (Table 1). This accords with other Thai native roosters: 215±80 µl (range 80-500 µl) for Pradu-hangdum (Khunkaew et al, 2021); 282 ± 89 µl for one-year-old Thai red junglefowl (Polsang et al, 2022), 255 ± 90 µl 7-8-month old and 208 ± 60 µl for 10-11-month old Thai native roosters (Inyawilert et al, 2019). Semen volumes may be age dependent: Thai native rooster, 8-13 months of age were not significant but tended to be lower in younger roosters (Chotesangasa, 2001).…”

“…Poultry semen has a low volume at high concentration and contains about 4-6 billion spermatozoa/ml. In the Thai native chicken, Pradu-hangdum, Khunkaew et al (2021) reported the sperm concentration of 5.24±0.58 billion sperm/ml. The mean semen volume of the 20-month-old Dang Surat rooster was 265±23 µl (Table 1).…”