The splicing factor 1–FLOWERING LOCUS M module spatially regulates temperature-dependent flowering by modulating FLOWERING LOCUS T and LEAFY expression

Lee, Keh Chien; Lee, Hee Tae; Jeong, Hwa Hyun; Park, Jae‐Hyeok; Kim, Young-Cheon; Lee, Jeong Hwan; Kim, Jeong-Kook

doi:10.1007/s00299-022-02881-y

Cited by 6 publications

(5 citation statements)

References 44 publications

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“…Quantitative gene expression was analyzed by qRT-PCR with SYBR Green PCR Master Mix as previously described [ 32 ]. The primers that have been successfully used for FT , SOC1 , TUB2 [ 29 ] and TSF [ 33 ] were used in this study for qRT-PCR analysis.…”

Section: Methodsmentioning

confidence: 99%

COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells

Feng

et al. 2022

IJMS

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RING-finger-type ubiquitin E3 ligase Constitutively Photomorphogenic 1 (COP1) and floral integrators such as FLOWERING LOCUS T (FT), TWIN SISTER OF FT (TSF) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) have been identified as regulators of stomatal movement. However, little is known about their roles and relationship in dark-induced stomatal closure. Here, we demonstrated that COP1 is required for dark-induced stomatal closure using cop1 mutant. The cop1 mutant closed stomata in response to exogenous nitric oxide (NO) but not hydrogen peroxide (H2O2), and H2O2 but not NO accumulated in cop1 in darkness, further indicating that COP1 acts downstream of H2O2 and upstream of NO in dark-induced stomatal closure. Expression of FT, TSF and SOC1 in wild-type (WT) plants decreased significantly with dark duration time, but this process was blocked in cop1. Furthermore, ft, tsf, and soc1 mutants accumulated NO and closed stomata faster than WT plants in response to darkness. Altogether, our results indicate that COP1 transduces H2O2 signaling, promotes NO accumulation in guard cells by suppressing FT, TSF and SOC1 expression, and consequently leads to stomatal closure in darkness. These findings add new insights into the mechanisms of dark-induced stomatal closure.

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Section: Methodsmentioning

confidence: 99%

COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells

Feng

et al. 2022

IJMS

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“…Plant homolog splicing factor 1 (AtSF1) has also been identified in Arabidopsis using forward and reverse genetic approaches [32,40]. A recent study showed that AtSF1 differentially binds to the BPS of different introns in FLM pre-mRNA in a temperature-dependent manner to regulate the production of the major functional FLM-β transcripts, thereby eventually affecting the temperature-responsive flowering (Figure 2, Table 1) [41]. Lee et al [42] reported that mutant lines harboring a deletion of the RNA recognition motif (RRM) domain of AtSF1 did not recover from the defect in the flowering time, suggesting that the RRM domain of AtSF1 is important for regulating the flowering time.…”

Section: Roles Of Snrnp-specific Proteinsmentioning

confidence: 99%

“…Lee et al [42] reported that mutant lines harboring a deletion of the RNA recognition motif (RRM) domain of AtSF1 did not recover from the defect in the flowering time, suggesting that the RRM domain of AtSF1 is important for regulating the flowering time. Genetic interactions and chromatin immunoprecipitation (ChIP) analyses revealed that the AtSF1-FLM module regulates temperature-dependent flowering by regulating the FLOWERING LOCUS T (FT) and LEAFY (LFY) expression in the leaf and shoot apex regions, respectively [41]. Furthermore, mutations in AtSF1 result in developmental abnormalities, including plastochron length, dwarfism, and hypersensitivity to abscisic acid during seed germination, heat stress, and chloroplast development under cold stress [32,[41][42][43], suggesting that AtSF1 is essential for various developmental processes and abiotic stresses.…”

Section: Roles Of Snrnp-specific Proteinsmentioning

confidence: 99%

“…Genetic interactions and chromatin immunoprecipitation (ChIP) analyses revealed that the AtSF1-FLM module regulates temperature-dependent flowering by regulating the FLOWERING LOCUS T (FT) and LEAFY (LFY) expression in the leaf and shoot apex regions, respectively [41]. Furthermore, mutations in AtSF1 result in developmental abnormalities, including plastochron length, dwarfism, and hypersensitivity to abscisic acid during seed germination, heat stress, and chloroplast development under cold stress [32,[41][42][43], suggesting that AtSF1 is essential for various developmental processes and abiotic stresses. SF1, U2AF35, and U2AF65 function at the early stage of pre-mRNA splicing, during which it binds the BPS, the PPT, and the 3' ss of the intron of the pre-mRNAs [2,29].…”

Section: Roles Of Snrnp-specific Proteinsmentioning

confidence: 99%

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Regulation of Flowering Time and Other Developmental Plasticities by 3’ Splicing Factor-Mediated Alternative Splicing in Arabidopsis thaliana

Lee,

Kim,

Kim

et al. 2023

Plants

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Plants, as sessile organisms, show a high degree of plasticity in their growth and development and have various strategies to cope with these alterations under continuously changing environments and unfavorable stress conditions. In particular, the floral transition from the vegetative and reproductive phases in the shoot apical meristem (SAM) is one of the most important developmental changes in plants. In addition, meristem regions, such as the SAM and root apical meristem (RAM), which continually generate new lateral organs throughout the plant life cycle, are important sites for developmental plasticity. Recent findings have shown that the prevailing type of alternative splicing (AS) in plants is intron retention (IR) unlike in animals; thus, AS is an important regulatory mechanism conferring plasticity for plant growth and development under various environmental conditions. Although eukaryotes exhibit some similarities in the composition and dynamics of their splicing machinery, plants have differences in the 3’ splicing characteristics governing AS. Here, we summarize recent findings on the roles of 3’ splicing factors and their interacting partners in regulating the flowering time and other developmental plasticities in Arabidopsis thaliana.

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“…In A. thaliana , the FT and LEAFY ( LFY ) genes are involved in the photoperiodic regulation of flowering initiation [ 35 ]. The ft mutants undergo flowering reversion under short-day conditions, and LF Y plays a similar role as a downstream gene of FT [ 36 ]. The SHORT VEGETATIVE PHASE ( SVP ) gene is a flowering repressor gene that causes flowering reversion when overexpressed in A. thaliana and Petunia hybrida [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis to Identify Genes Related to Flowering Reversion in Tomato

Sun

Yang²,

Chen³

et al. 2022

IJMS

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Flowering reversion is a common phenomenon in plant development in which differentiated floral organs switch from reproductive growth to vegetative growth and ultimately form abnormal floral organs or vegetative organs. This greatly reduces tomato yield and quality. Research on this phenomenon has recently increased, but there is a lack of research at the molecular and gene expression levels. Here, transcriptomic analyses of the inflorescence meristem were performed in two kinds of materials at different developmental stages, and a total of 3223 differentially expressed genes (DEGs) were screened according to the different developmental stages and trajectories of the two materials. The analysis of database annotations showed that these DEGs were closely related to starch and sucrose metabolism, DNA replication and modification, plant hormone synthesis and signal transduction. It was further speculated that tomato flowering reversion may be related to various biological processes, such as cell signal transduction, energy metabolism and protein post-transcriptional regulation. Combined with the results of previous studies, our work showed that the gene expression levels of CLE9, FA, PUCHI, UF, CLV3, LOB30, SFT, S-WOX9 and SVP were significantly different in the two materials. Endogenous hormone analysis and exogenous hormone treatment revealed a variety of plant hormones involved in flowering reversion in tomato. Thus, tomato flowering reversion was studied comprehensively by transcriptome analysis for the first time, providing new insights for the study of flower development regulation in tomato and other plants.

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The splicing factor 1–FLOWERING LOCUS M module spatially regulates temperature-dependent flowering by modulating FLOWERING LOCUS T and LEAFY expression

Cited by 6 publications

References 44 publications

COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells

COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells

Regulation of Flowering Time and Other Developmental Plasticities by 3’ Splicing Factor-Mediated Alternative Splicing in Arabidopsis thaliana

Transcriptome Analysis to Identify Genes Related to Flowering Reversion in Tomato

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