The sputum microbiome associated with different sub-types of AECOPD in a Chinese cohort

Wang, Juan; Chai, Jianmin; L, Sun; Zhao, Jiangchao; Chang, Chun

doi:10.1186/s12879-020-05313-y

Cited by 20 publications

(18 citation statements)

References 39 publications

(57 reference statements)

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“…Proteobacteria and its members such as Haemophilus were associated with the onset of AECOPD [ 27 ]. In the present study, the most dominant phylum in sputum samples from the stable COPD group was Firmicutes, consistent with previous findings [ 28 , 29 ].…”

Section: Discussionsupporting

confidence: 93%

Characteristics of the sputum microbiome in COPD exacerbations and correlations between clinical indices

Qiao

Luo

et al. 2022

J Transl Med

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Background Chronic obstructive pulmonary disease (COPD) is a prevalent, progressive respiratory disease, and acute exacerbations of COPD (AECOPD) can accelerate the deterioration of the disease. Increasing evidence suggests that airway bacterial dysbiosis is associated with AECOPD. However, the exact relationship between changes in the sputum microbiome during AECOPD and clinical indices remains unclear. Methods In this study, a total of 76 sputum samples were collected from patients with AECOPD (n = 28), stable COPD (n = 23), recovery (n = 15) and healthy controls (HCs; n = 10). The sputum microbiome profile was analysed by sequencing the V3‑V4 amplicon of the 16S rRNA (ribosomal RNA) gene. Results The bacterial diversity (Shannon and Simpson’s index) was found to be significantly decreased in the AECOPD and recovery groups when compared to that in the stable COPD and HC groups. The most dominant phylum identified in the sputum samples of AECOPD patients was Proteobacteria, accounting for 30% of the microbiome. Compared to the stable COPD groups, the relative abundances of Firmicutes and Bacteroidetes were decreased, whereas those of Proteobacteria and Actinobacteria were increased in AECOPD patients. Furthermore, discriminative bacteria, such as Haemophilus, were identified as being specific taxa in AECOPD patients. Functional analysis showed that genes involved in membrane transport and signal transduction metabolism were enriched in the AECOPD group. Importantly, the proportions of Veillonella were positively correlated with lung function, and Staphylococcus was positively correlated with inflammatory indices. Conclusion Our study revealed variations in the sputum microbiome of AECOPD (based on composition and function) in a Chinese cohort and highlighted its correlation to clinical indices. These results indicated that microbial dysbiosis may contribute to disease progression and provide microbial biomarkers for the diagnosis of AECOPD.

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Section: Discussionsupporting

confidence: 93%

Characteristics of the sputum microbiome in COPD exacerbations and correlations between clinical indices

Qiao

Luo

et al. 2022

J Transl Med

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“…When the stable and exacerbated states of disease were compared in this study a higher abundance of the Firmicutes phylum (2% higher in the exacerbated state) and lower abundances of the Proteobacteria (2% higher in the exacerbated state), Actinobacteria (3% higher in the exacerbated state) and Bacteroidetes phyla (2% higher in the exacerbated state) was observed. Previous studies (all conducted using sputum specimens) that have compared the two diseases states in COPD have observed the same trend, where one of the phyla shows an increased prevalence and the other phyla showed a decreased prevalence in the exacerbated state, however, none of these studies reported the percentage increase 66 – 71 . In most of these studies, Proteobacteria increased, however, two studies [Jubinville et al .…”

Section: Discussionmentioning

confidence: 85%

“…(2020)], showed an increased prevalence of Firmicutes (as observed in this study). These studies had a variety of different sample sizes (ranging from nine participants to 281 participants), were conducted in USA, Europe and China and used different sequencing technologies (454 sequencing, MiSeq sequencing and PhyloChip) 66 – 71 .…”

Section: Discussionmentioning

confidence: 99%

Lung microbiome of stable and exacerbated COPD patients in Tshwane, South Africa

Mahomed

Peters

Allam

et al. 2021

Sci Rep

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Chronic obstructive pulmonary disease (COPD) is characterised by the occurrence of exacerbations triggered by infections. The aim of this study was to determine the composition of the lung microbiome and lung virome in patients with COPD in an African setting and to compare their composition between the stable and exacerbated states. Twenty-four adult COPD patients were recruited from three hospitals. Sputum was collected and bacterial DNA was extracted. Targeted metagenomics was performed to determine the microbiome composition. Viral DNA and RNA were extracted from selected samples followed by cDNA conversion. Shotgun metagenomics sequencing was performed on pooled DNA and RNA. The most abundant phyla across all samples were Firmicutes and Proteobacteria. The following genera were most prevalent: Haemophilus and Streptococcus. There were no considerable differences for alpha and beta diversity measures between the disease states. However, a difference in the abundances between disease states was observed for: (i) Serratia (3% lower abundance in exacerbated state), (ii) Granulicatella (2.2% higher abundance in exacerbated state), (iii) Haemophilus (5.7% higher abundance in exacerbated state) and (iv) Veillonella (2.5% higher abundance in exacerbated state). Virome analysis showed a high abundance of the BeAn 58058 virus, a member of the Poxviridae family, in all six samples (90% to 94%). This study is among the first to report lung microbiome composition in COPD patients from Africa. In this small sample set, no differences in alpha or beta diversity between stable and exacerbated disease state was observed, but an unexpectedly high frequency of BeAn 58058 virus was observed. These observations highlight the need for further research of the lung microbiome of COPD patients in African settings.

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“…AECOPD refers the acute situation of COPD featured by the worsening of the patient's respiratory symptoms. [ 16 ] Bacterial infection is a leading reason for AECOPD, and antibiotic treatment is necessary for the patients. In addition to antibacterial treatment, the appropriate NIPPV treatment could shorten the hospitalization time, improve the pain, and reduce the need of endotracheal intubation.…”

Section: Discussionmentioning

confidence: 99%

The predictive value of serum procalcitonin for non-invasive positive pressure ventilation in the patients with acute exacerbation of chronic obstructive pulmonary disease

Liu¹,

Xiao³

et al. 2021

Medicine

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This study aimed to estimate the value of serum procalcitonin (PCT) for non-invasive positive pressure ventilation (NIPPV) prediction in the patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). A total of 220 AECOPD patients were divided into NIPPV group (n = 121) and control group (routine treatment, n = 99) based on the routine standards and physicians’ experience. Logistic regression analysis was performed to identify the independent factors for NIPPV treatment. Additionally, the predictive values of the factors were measured through receiver operation characteristic (ROC) curve. NIPPV group and control group showed significant differences in respiratory rate (RR), PaO 2 , PaCO 2 , pH, oxygenation index, erythrocyte sedimentation rate (ESR), neutrophil, CRP (C-reaction protein), and PCT ( P < .05 for all). PCT, CRP, PaCO 2 , RR, and neutrophil were independently correlated with NIPPV treatment in AECOPD. ROC curve showed that PCT had superior predictive value, with AUC of 0.899, the sensitivity of 86%, and the specificity of 91.9%. The cut-off value of serum PCT for NIPPV prediction was 88.50 ng/l. AECOPD patients who require NIPPV treatment frequently have high levels of PCT, CRP, PaCO 2 , RR and neutrophil. Serum PCT may be employed as an indicator for NIPPV treatment in AECOPD patients.

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The sputum microbiome associated with different sub-types of AECOPD in a Chinese cohort

Cited by 20 publications

References 39 publications

Characteristics of the sputum microbiome in COPD exacerbations and correlations between clinical indices

Characteristics of the sputum microbiome in COPD exacerbations and correlations between clinical indices

Lung microbiome of stable and exacerbated COPD patients in Tshwane, South Africa

The predictive value of serum procalcitonin for non-invasive positive pressure ventilation in the patients with acute exacerbation of chronic obstructive pulmonary disease

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