The stabilization of electrospun chitosan nanofibers by reversible acylation

Wu, Chaoxi; Su, Hengjie; Tang, Shunqing; Bumgardner, Joel D.

doi:10.1007/s10570-014-0306-3

Cited by 19 publications

(22 citation statements)

References 16 publications

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“…The addition of the butyric fatty acid molecule to the outside of the chitosan nanofibers increases the hydrophobic characteristics of the membrane and retains the nanofiber morphology when immersed in aqueous solutions [54].…”

Section: Preparation Of Chitosan Membranesmentioning

confidence: 99%

Evaluation of Bone Regeneration of Simvastatin Loaded Chitosan Nanofiber Membranes in Rodent Calvarial Defects

Ghadri¹,

Anderson²,

Adatrow³

et al. 2018

JBNB

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Chitosan nanofiber membranes have been known to have a high degree of biocompatibility and support new bone formation with controllable biodegradation. The surface area of these membranes may allow them to serve as local delivery carriers for different biologic mediators. Simvastatin, a drug commonly used for lowering cholesterol, has demonstrated promising bone regenerative capability. The aim of this study was to evaluate simvastatin loaded chitosan nanofiber membranes for guided bone regeneration (GBR) applications and their ability to enhance bone formation in rat calvarial defects. Nanofibrous chitosan membranes with random fiber orientation were fabricated by electrospinning technique and loaded with 0.25 mg of simvastatin under sterile conditions. One membrane was implanted subperiosteally to cover an 8 mm diameter critical size calvarial defect. Two groups: 1) Control: non-loaded chitosan membranes; 2) Experimental: chitosan membranes loaded with 0.25 mg of simvastatin were evaluated histologically and via micro-computed tomography (micro-CT) for bone formation at 4 and 8 weeks time points (n = 5/group per time point). Both groups exhibited good biocompatibility with only mild or moderate inflammatory response during the healing process. Histologic and micro-CT evaluations confirmed bone formation in calvarial defects as early as 4 weeks using control and experimental membranes. In addition, newly-formed bony bridges consolidating calvarial defects histologically along with partial radiographic defect coverage were observed at 8 weeks in both groups.

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Section: Preparation Of Chitosan Membranesmentioning

confidence: 99%

Evaluation of Bone Regeneration of Simvastatin Loaded Chitosan Nanofiber Membranes in Rodent Calvarial Defects

Ghadri¹,

Anderson²,

Adatrow³

et al. 2018

JBNB

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“…While chitosan is soluble in acidic aqueous solution, technical difficulties related to the electrospinning of uniform fibers arise due to repulsive interactions between the cationic sub-units of chitosan that attenuate chain entanglement effects [4,[19][20][21]25]. The judicious choice of additive components offers a solution to offset such charge repulsion effects during electrospinning [4,7,[26][27][28][29][30]. For example, Burns et al reported the use of trifluoroacetic acid (TFA) and hydroxypropyl β-cyclodextrin (HPCD) as additives to assist in the electrospinning of chitosan nanofibers [31].…”

Section: Introductionmentioning

confidence: 99%

A Spectroscopic Study of Solid-Phase Chitosan/Cyclodextrin-Based Electrospun Fibers

Chen

Wilson

2019

Fibers

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In this study, chitosan (chi)/hydroxypropyl-β-cyclodextrin (HPCD) 2:20 and 2:50 Chi:HPCD fibers were assembled via an electrospinning process that contained a mixture of chitosan and HPCD with trifluoroacetic acid (TFA) as a solvent. Complementary thermal analysis (thermal gravimetric analysis (TGA)/differential scanning calorimetry (DSC)) and spectroscopic methods (Raman/IR/NMR) were used to evaluate the structure and composition of the fiber assemblies. This study highlights the multifunctional role of TFA as a solvent, proton donor and electrostatically bound pendant group to chitosan, where the formation of a ternary complex occurs via supramolecular host–guest interactions. This work contributes further insight on the formation and stability of such ternary (chitosan + HPCD + solvent) electrospun fibers and their potential utility as “smart” fiber coatings for advanced applications.

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“…ESCMs were electrospun using an in‐house box at room temperature and 40–60% humidity 2,19,20 . Chitosan (5.5% w/v) was dissolved in TFA‐DCM (70–30), loaded into 10 ml syringe with 20‐gauge blunt needle, and electrospun at 27 kV onto aluminum foil covered collector plate, rotating at ~8.4 rpm.…”

Section: Methodsmentioning

confidence: 99%

“…Membranes were punched into 1 cm diameter disks, soaked in pyridine‐anhydride (AA or HA) (v/v) (50–50) solution at 5 mg/ml for 1.5 h, 2 and then rinsed for 24 h in 1L MilliQ water (18MΩ@25°, Integral 15, Millipore) to remove the unreacted reagents. Water was changed every 24 h for 3 days, and membranes were frozen and lyophilized (FreeZone 2.5, Labconco Corp).…”

Section: Methodsmentioning

confidence: 99%

“…Electrospun chitosan membranes (ESCM) modified with short‐chain fatty acids (FAs) have several advantages over other ESCMs, such as, not swelling and retaining nanofibrous structure in aqueous environments, and being biodegradable, making them a promising candidate for guided bone regeneration (GBR) applications 1‐3 . Retention of nanofiber structure is important for providing increased surface area for drug delivery, promoting cell attachment and growth, and enabling exchange of nutrients between osseous and epithelial tissues, while remaining cell occlusive 4,5 …”

Section: Introductionmentioning

confidence: 99%

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Simvastatin loaded chitosan guided bone regeneration membranes stimulate bone healing

Murali

Guerra

Ghadri

et al. 2021

J of Periodontal Research

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Background and Objective Electrospun chitosan membranes (ESCM) modified with short‐chain fatty acids have the ability to control the release of simvastatin (SMV), an anti‐cholesterol drug with osteogenic potential, for guided bone regeneration (GBR) applications. This study evaluated in vivo osteogenic effects of rapid short release of SMV (4 weeks) vs long sustained release (8 weeks) from acetic anhydride (AA)—and hexanoic anhydride (HA)‐modified ESCMs, respectively. Methods AA ESCMs loaded with 10 or 50 µg SMV and HA ESCMs loaded with 50 µg SMV were evaluated for biocompatibility and bone formation at 4 and 8 weeks, in 5 mm critical size rat calvarial defects, using histological evaluation and micro‐CT analysis. Results No severe inflammatory response was noticed around the ESCMs. Less hydrophobic AA membranes showed signs of resorption by week 4 and were almost completely resorbed by week 8 whereas the more hydrophobic HA membranes resorbed slowly, remaining intact over 8 weeks. In micro‐CT analysis, 10 µg SMV‐loaded AA membranes did not show significant bone formation as compared to non‐loaded AA membranes at either evaluation time points. 50 µg SMV‐loaded AA membranes stimulated significantly more bone formation than non‐loaded AA membranes by week 4 (%bone = 31.0 ± 5.9% (AA50) vs 18.5 ± 13.7% (AA0)) but showed no difference at week 8. HA membranes with 50 µg SMV showed significantly more bone formation as compared to corresponding non‐loaded membranes by week 8 (%bone = 61.7 ± 8.9% (HA50) vs 33.9 ± 29.7% (HA0)), though such an effect was not significant at week 4. Conclusion These results indicate that modified ESCMs may be used to control the release of SMV and promote bone healing in GBR applications.

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The stabilization of electrospun chitosan nanofibers by reversible acylation

Cited by 19 publications

References 16 publications

Evaluation of Bone Regeneration of Simvastatin Loaded Chitosan Nanofiber Membranes in Rodent Calvarial Defects

Evaluation of Bone Regeneration of Simvastatin Loaded Chitosan Nanofiber Membranes in Rodent Calvarial Defects

A Spectroscopic Study of Solid-Phase Chitosan/Cyclodextrin-Based Electrospun Fibers

Simvastatin loaded chitosan guided bone regeneration membranes stimulate bone healing

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