The Stabilization of Lactic Acid Bacteria, Bacillus polyfermenticus KJS-2

Abstract

Bacillus polyfermenticus KJS-2 (Bp2) was isolated from Bispan Ⓡ , a commercially available probiotics consisting of more than 4 strains. The objective of this study was to investigate the effect of three-layer coating on the stabilty of Bp2. Bp2 was microencapsulated with sodium alginate using an air atomizer. The Bp2 loaded pellets were also coated with HFP-chitosan and HPMCP for oral delivery system. When compared to the uncoated Bp2, the survival of the three-layer coated Bp2 increased to approximately 63% (p<0.01) in a 30% ethanol solution, 54% (p<0.05) in an artificial gastric juice (pH 2), and 53% (p<0.05) in the bile acid (pH 5). When coated beads were stored at 100 o C and 130 o C, Bp2 in coated beads was very stable (p<0.01) compared to uncoated Bp2.

Isolation and characterization of surfactin produced by Bacillus polyfermenticus KJS-2

“…BP-KJS-2 was isolated from B. polyfermenticus n. sp, which is comprised of several strains of B. polyfermenticus (Kim et al, 2008). The basal medium used for the growth of bacteria was tryptone soy broth (TSB …”