The Standard Romanowsky-Giemsa Stain in Histology

Wittekind, D; Schulte, Erik; Schmidt, Gudrun; Frank, GÜNter

doi:10.3109/10520299109109989

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…Each question was answered on a scale of 0 to 4, and the total score was calculated. Higher FACE-Q scores in the form of Rasch-transformed responses represent greater objective values for the subjective variables [36]. In case 2, the reconstructed nasal skeleton was examined using CT scanning (Planmeca, Finland) two years after surgery.…”

Section: Assessment Of Resultsmentioning

confidence: 99%

Tissue engineering approach to the creation of grafting material for rhinoplasty: clinical сase reports

Karpiuk,

Gilevich,

Perova

et al. 2024

Explor BioMat-X

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The use of autologous cartilage and bone grafts remains the gold standard in augmentation rhinoplasty performed to reconstruct of the nasal dorsum. Meanwhile, limited number of available sources, donor site morbidity, and unpredictable graft resorption represent significant disadvantages of autografting. The aim of this study is to test combination of autologous stromal vascular fraction (SVF) and commercially available bone substitutes (BSs) as new tissue-engineered grafting material (GM) for rhinoplasty. A series of consecutive cases includes four adult patients who underwent rhinoplasty to correct saddle nose deformity (SND) using the new graft technique. SVF was isolated from liposuction aspirate using standard methodology of enzymatic digestion. Two types of BSs were combined with SVF: Bio-Oss granules to create a moldable graft (M-graft), and block-shaped BoneMedik-S to create rigid grafts (R-grafts). The moderate SND was treated using an M-graft. In case of major or complex SND, the nasal dorsum was reconstructed with dorso-columellar L-shaped framework made of R-grafts. The results were evaluated over a period of 6 months to 3 years postoperatively using photogrammetry and FACE-Q appearance appraisal scales. Computerised tomography (CT) scanning of the reconstructed nose and histological analysis of grafted material were also carried out. No complications were observed. The photograms show the restoration of the correct contour of the nose. FACE-Q appraisal scale scores increased significantly, including satisfaction with nose appearance, psychological well-being, and social function. In CT evaluation, there was no substantial resorption or warping of the grafts. Histological findings show osteogenic remodeling of the grafted material. Thus, combining autologous SVF with BSs is a promising strategy for developing rhinoplasty GM.

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Section: Assessment Of Resultsmentioning

confidence: 99%

Tissue engineering approach to the creation of grafting material for rhinoplasty: clinical сase reports

Karpiuk,

Gilevich,

Perova

et al. 2024

Explor BioMat-X

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“…When Giemsa is used for staining bacteria in tissue section fixed by formaldehyde, the organism stains purple and pink cytoplasm will be seen (Kiernan, 2008). Wittekind et al (1991) found that Giemsa stain seems suitable to replace the Gomori-type trichrome stains under appropriate staining conditions. The staining result depends on many factors such as pH and differentiation and this is in line with current study (Wolf-Dieter, 2006).…”

Section: Discussionmentioning

confidence: 96%

Comparison between Giemsa and Van Geison stains in demonstration of collagen fibers (Kosti-2016)

Mudasir¹,

Adil²,

Edriss³

2016

Afr. J. Biotechnol.

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Trichrome stain (such as Van Geison) is usually used in histopathology laboratory for demonstration of collagenic fibers. Lack of selectivity and tendency of stain to fade makes van Gieson not ideal for collagen demonstration. This study was aimed to compare between Giemsa's and van Gieson's stains in collagen fibers demonstration. Twenty biopsies were obtained from rabbit's skin after anesthesia by chloroform and immediately fixed by 10% neutral buffered formalin for 48 h. Then samples were processed using tissue processing machine and sectioned by rotary microtome. Two hundred (200) tissue sections of 5 micron thickness were prepared. A 100 tissue sections was stained by Van Geison and another 100 tissue sections stained by Giemsa. The stained section was compared with illustrated photomicrographs in order to assess staining quality. Best collagen staining quality was obtained by Van Geison's 60 (60%) and 40 (40%), mean 1.40, followed by Giemsa's stain excellent 55 (55%) and good 45 (45%), mean 1.45. Conclusively, Van Geison's is superior but Giemsa stain is rapid, sensitive without fading tendency, easy to perform and low cost and can be used as special stain under optimized conditions.

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“…In 1892, Dimitri Romanowsky described a technique for staining parasites in blood smear. His method employs the use of methylene blue and its products of oxidation in combination with eosin to stain the blood smear, producing a red nuclei 13, 21 . The method was modified by Wright in 1902 and Giemsa in 1902 and these methods have significantly improved the staining of parasites.…”

Section: Introductionmentioning

confidence: 99%

Histological stains and their application in teaching and research

Dibal

Garba

Jacks

2022

Asian J. Health Sci.

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This review aimed to highlight the historical perspectives focused on histological stains and their application in teaching and research. While early histologists used indigenous substances like alizarin, carmine, and saffron to stain tissues, modern histologists utilize techniques that study cellular organelles and substances within tissues. Despite the advent of new staining techniques, the modification of old ones, and decrease in the use of natural dyes in the 20 th century, some of the stains used in the past are still very much in use. These techniques are used in teaching and research for the demonstration and display of the different substances within tissues. These include the Giemsa stain for parasites and fungi, the Trichrome stain for collagen, oil red O stain for lipids, periodic acid Schiff for glycogen, Crystal violet for Nissl granules, Golgi stain for neuronal cells, as well as routine hematoxylin, and eosin (H&E) for a general histology. Histological stains can be used to teach histology, cellular pathology, microbiology, and parasitology. They can also be used to study the rate of progression of disease or the healing process of diseased conditions.

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The Standard Romanowsky-Giemsa Stain in Histology

Cited by 6 publications

References 17 publications

Tissue engineering approach to the creation of grafting material for rhinoplasty: clinical сase reports

Tissue engineering approach to the creation of grafting material for rhinoplasty: clinical сase reports

Comparison between Giemsa and Van Geison stains in demonstration of collagen fibers (Kosti-2016)

Histological stains and their application in teaching and research

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