The strengths of in vivo magnetic resonance imaging (MRI) to study environmental adaptational physiology in fish

Linden, Annemie Van der; Verhoye, Marleen; Pörtner, Hans‐Otto; Bock, Christian

doi:10.1007/s10334-004-0078-0

Cited by 28 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…External flows Review. Hydrodynamic aspects of fish olfaction J. P. L. Cox 589 could be monitored by laser Doppler velocimetry (Kux et al 1977(Kux et al , 1978(Kux et al , 1988, particle image velocimetry (Gharib & Daribi 2000) or (possibly) by schlieren and shadowgraph techniques (Settles 2001) and internal flows by magnetic resonance imaging (Bock et al 2002;van der Linden et al 2004;Pohlmann et al 2007). These experiments would not be trivial, however, especially if one were keen not to cause the fish distress.…”

Section: Future Directionsmentioning

confidence: 99%

Hydrodynamic aspects of fish olfaction

Cox

2008

J. R. Soc. Interface.

View full text Add to dashboard Cite

Flow into and around the olfactory chamber of a fish determines how odorant from the fish's immediate environment is transported to the sensory surface (olfactory epithelium) lining the chamber. Diffusion times in water are long, even over comparatively short distances (millimetres). Therefore, transport from the external environment to the olfactory epithelium must be controlled by processes that rely on convection (i.e. the bulk flow of fluid). These include the beating of cilia lining the olfactory chamber and the relatively inexpensive pumping action of accessory sacs. Flow through the chamber may also be induced by an external flow. Flow over the olfactory epithelium appears to be laminar. Odorant transfer to the olfactory epithelium may be facilitated in several ways: if the olfactory organs are mounted on stalks that penetrate the boundary layer; by the steep velocity gradients generated by beating cilia; by devices that deflect flow into the olfactory chamber; by parallel arrays of olfactory lamellae; by mechanical agitation of the chamber (or olfactory stalks); and by vortices. Overall, however, our knowledge of the hydrodynamics of fish olfaction is far from complete. Several areas of future research are outlined.

show abstract

Section: Future Directionsmentioning

confidence: 99%

Hydrodynamic aspects of fish olfaction

Cox

2008

J. R. Soc. Interface.

View full text Add to dashboard Cite

show abstract

“…tilapia ( Oreochromis mossambicus ) [7], Crassius X [8] and Atlantic cod [9], as well as MRI studies of large fish e.g. carp [10] and eelpout [11]. In contrast, there has been limited use of MRI techniques in the much smaller (~2 cm) and bio-medically important zebrafish [12], [13], [14], [15], [16].…”

Section: Introductionmentioning

confidence: 99%

Rapid and recoverable in vivo magnetic resonance imaging of the adult zebrafish at 7T

Merrifield

Mullin

Gallagher

et al. 2017

Magnetic Resonance Imaging

View full text Add to dashboard Cite

Increasing scientific interest in the zebrafish as a model organism across a range of biomedical and biological research areas raises the need for the development of in vivo imaging tools appropriate to this subject. Development of the embryonic and early stage forms of the subject can currently be assessed using optical based techniques due to the transparent nature of the species at these early stages. However this is not an option during the juvenile and adult stages when the subjects become opaque. Magnetic resonance imaging (MRI) techniques would allow for the longitudinal and non-invasive assessment of development and health in these later life stages. However, the small size of the zebrafish and its aquatic environment represent considerable challenges for the technique. We have developed a suitable flow cell system that incorporates a dedicated MRI imaging coil to solve these challenges. The system maintains and monitors a zebrafish during a scan and allows for it to be fully recovered. The imaging properties of this system compare well with those of other preclinical MRI coils used in rodent models. This enables the rapid acquisition of MRI data which are comparable in terms of quality and acquisition time. This would allow the many unique opportunities of the zebrafish as a model organism to be combined with the benefits of non-invasive MRI.

show abstract

“…Although MRI has been conducted on finfish previously (e.g., Bock, Sartoris & Pörtner, 2002; Bock, Sartoris, Wittig, et al., 2002; Collewet et al., 2013; Howell et al., 1996; Lannig et al., 2004; Mark et al., 2002; Pörtner et al., 2004; Rogers et al., 2008; Simões et al., 2012; Van der Linden et al., 2004; Wu et al., 2015), including for the purposes of diagnosing musculoskeletal pathology (Torpy et al., 2023), it has not yet been applied to the diagnosis of musculoskeletal pathology in commercially farmed finfish. The aim of the current study was to use MRI to assess peri‐vertebral soft‐tissue pathology in farmed New Zealand Chinook salmon and, in conjunction with histology, evaluate its association with spinal curvature.…”

Section: Introductionmentioning

confidence: 99%

Magnetic resonance imaging shows spinal curvature in Chinook salmon (Oncorhynchus tshawytscha) is associated with chronic inflammation of peri‐vertebral soft tissues

Lovett,

Firth,

Perrott

et al. 2023

Journal of Fish Diseases

View full text Add to dashboard Cite

Chinook salmon (Oncorhynchus tshawytscha) farmed in New Zealand are known to develop abnormal spinal curvature late in seawater production. Its cause is presently unknown, but there is evidence to suggest a neuromuscular pathology. Using magnetic resonance imaging (MRI), we evaluated the relationship between soft tissue pathology and spinal curvature in farmed Chinook salmon. Regions of interest (ROIs) presenting as pathologic MRI signal hyper‐intensity were identified from scans of 24 harvest‐sized individuals: 13 with radiographically‐detectable spinal curvature and 11 without. ROIs were excised from individuals using anatomical landmarks as reference points and histologically analysed. Pathologic MRI signal was observed more frequently in individuals with radiographic curvature (92%, n = 12) than those without (18%, n = 2), was localized to the peri‐vertebral connective tissues and musculature, and presented as three forms: inflammation, fibrosis, or both. These pathologies are consistent with a chronic inflammatory process, such as that observed during recovery from a soft tissue injury, and suggest spinal curvature in farmed Chinook salmon may be associated with damage to and/or compromised integrity of the peri‐vertebral soft tissues. Future research to ascertain the contributing factors is required.

show abstract

The strengths of in vivo magnetic resonance imaging (MRI) to study environmental adaptational physiology in fish

Cited by 28 publications

References 37 publications

Hydrodynamic aspects of fish olfaction

Hydrodynamic aspects of fish olfaction

Rapid and recoverable in vivo magnetic resonance imaging of the adult zebrafish at 7T

Magnetic resonance imaging shows spinal curvature in Chinook salmon (Oncorhynchus tshawytscha) is associated with chronic inflammation of peri‐vertebral soft tissues

Contact Info

Product

Resources

About