The stress granule component G3BP is a novel interaction partner for the nuclear shuttle proteins of the nanovirus pea necrotic yellow dwarf virus and geminivirus abutilon mosaic virus

Rasputin a decade on and more promiscuous than ever? A review of G3BPs

“…The nuclear shuttle protein (NSP) of begomo ablution mosaic virus (AbMV), has a FVSF motif which interacts with the G3BP-like protein of Arabidopsis thaliana (AtG3BP) which is responsible for SGs assembly in plants during cellular stress and thereby inhibiting SGs. Moreover, the NSP of pea necrotic yellow dwarf virus (PNYDV) harbors a FNSGF motif which also interacts with AtG3BP [118]. These findings support the speculation that SGs assembly is conserved in plants and mammalian cells and plant viruses also inhibit SGs formation by interacting with G3BPs to invade host cellular responses in response to infection.…”

Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

“…This protein is closely related to the NUCLEAR TRANSPORT FACTOR2 (NTF2) family in Arabidopsis. This G3BP-like NTF2-RRM aggregates into SGs and interacts with the nuclear shuttle protein of the begomovirus Abutilon mosaic virus and Pea necrotic yellow dwarf virus upon heat stress (Krapp et al, 2017). The roles of plant G3BP-like proteins in SG nucleation and mRNA stabilization await further studies.…”

“…As such, G3BP1 is targeted and sequestered by several viral proteins to prevent SG assembly/sequestration of viral RNAs during infection (Reineke and Lloyd, 2013). The first plant G3BP-like protein was isolated from N. benthamiana together with other SG components as targets of the viral nonstructural protein3 of Semliki Forest virus (Krapp et al, 2017). This protein is closely related to the NUCLEAR TRANSPORT FACTOR2 (NTF2) family in Arabidopsis.…”

The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion

“…A peroxisome organelle marker PTS1-RFP was available from the Nottingham Arabidopsis Stock Centre (NASC), stock number CD3-983. Plasmids containing the pUBQ10::3xHA-TagRFP-AtG8a sequences were kindly provided by Prof. Dr. Daniel Van Damme (VIB-UGhent Center for Plant Systems Biology, Gent, Belgium) [57] and plasmids containing G3BP-RFP sequences were kindly provided by the lab of Prof. Dr. Peter Tompa (VIB-VUB Structural Biology Research Center, Brussels, Belgium) [58]. All vectors were introduced into Agrobacterium tumefaciens (strains C58C1 PMP90 RifR, GV3101 or UIA143) by electroporation.…”