The structural alteration of gut microbiota in low-birth-weight mice undergoing accelerated postnatal growth

Wang, Jingjing; Tang, Huang; Wang, Xiaoxin; Zhang, Xu; Zhang, Chenhong; Zhang, Menghui; Zhao, Yuwu; Zhao, Liping; Shen, Jian

doi:10.1038/srep27780

Cited by 23 publications

(21 citation statements)

References 67 publications

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“…A lower relative abundance of Firmicutes was harbored by LBW piglets on D3 and D7, which was in agreement with the fact that the relative abundance of Firmicutes decreased in the placenta from LBW infants ( Zheng et al, 2015 ). Also, in the present study, LBW piglets had a lower abundance of Lactobacillus and Streptococcus but a higher abundance of Fusobacterium , which was consistent with previous studies in LBW infants ( Arboleya et al, 2012a ; Zheng et al, 2015 ) and rodents ( Wang et al, 2016 ). LBW piglets are well recognized as being more susceptible to GIT defects and various diseases ( Li et al, 2017 ; Wang et al, 2017 ).…”

Section: Discussionsupporting

confidence: 93%

Differences in the Gut Microbiota Establishment and Metabolome Characteristics Between Low- and Normal-Birth-Weight Piglets During Early-Life

Huang

Jiang

et al. 2018

Front. Microbiol.

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Low-birth-weight (LBW) piglets are at a high-risk for postnatal growth failure, mortality, and metabolic disorders later in life. Early-life microbial exposure is a potentially effective intervention strategy for modulating the health and metabolism of the host. Yet, it has not been well elucidated whether the gut microbiota development in LBW piglets is different from their normal littermates and its possible association with metabolite profiles. In the current study, 16S rRNA gene sequencing and metabolomics was used to investigate differences in the fecal microbiota and metabolites between LBW and normal piglets during early-life, including day 3 (D3), 7 (D7), 14 (D14), 21 (D21, before weaning), and 35 (D35, after birth). Compared to their normal littermates, LBW piglets harbored low proportions of Faecalibacterium on D3, Flavonifractor on D7, Lactobacillus, Streptococcus, and Prevotella on D21, as well as Howardella on D21 and D35. However, the abundance of Campylobacter on D7 and D21, Prevotella on D14 and D35, Oscillibacter and Moryella on D14 and D21, and Bacteroides on D21 was significantly higher in LBW piglets when compared with normal piglets. The results of the metabolomics analysis suggested that LBW significantly affected fecal metabolites involved in fatty acid metabolism (e.g., linoleic acid, α-linolenic acid, and arachidonic acid), amino acid metabolism (e.g., valine, phenylalanine, and glutamic acid), as well as bile acid biosynthesis (e.g., glycocholic acid, 25-hydroxycholesterol, and chenodeoxycholic acid). Spearman correlation analysis revealed a significant negative association between Campylobacter and N1-acetylspermine on D7, Moryella and linoleic acid on D14, Prevotella and chenodeoxycholic acid on D21, and Howardella and phenylalanine on D35, respectively. Collectively, LBW piglets have a different gut bacterial community structure when compared with normal-birth-weight (NBW) piglets during early-life, especially from 7 to 21 days of age. Also, a distinctive metabolic status in LBW piglets might be partly associated with the altered intestinal microbiota. These findings may further elucidate the factors potentially associated with the impaired growth and development of LBW piglets and facilitate the development of nutritional interventions.

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Section: Discussionsupporting

confidence: 93%

Differences in the Gut Microbiota Establishment and Metabolome Characteristics Between Low- and Normal-Birth-Weight Piglets During Early-Life

Huang

Jiang

et al. 2018

Front. Microbiol.

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“…In this regard, a significant elevation of Enterorhabdus, and appearance of Facklamia and Veillonella was found in this group of rats. Enterorhabdus has been implicated in the development of obesity (Wang et al 2016), autism spectrum disorders (de Theije et al 2014), and is predominant in prediabetic patients (Yang et al 2015). Facklamia, alpha-haemolytic bacteria, have been shown to cause bacteraemia (Rahmati et al 2017), acute cystitis and sepsis (Mostafa et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Prevention of excessive exercise‐induced adverse effects in rats withBacillus subtilisBSB3

et al. 2019

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Aims To characterize efficacy of the Bacillus subtilis BSB3 (BSB3) strain in the prevention of excessive exercise‐induced side effects and in maintaining stability of the gut microbiota. Methods and Results Rats were pretreated by oral gavage with B. subtilis BSB3 (BSB3) or with phosphate‐buffered saline (PBS) twice a day for 2 days, and were either exposed forced treadmill running or remained sedentary. Histological analysis of intestine, immunofluorescence staining of tight junction (TJ) proteins, serum lipopolysaccharide and intestinal fatty acid‐binding protein assay, culture‐based analysis and pyrosequencing for the gut microbiota were performed for each rat. Forced running resulted in a substantial decrease in intestinal villi height and total mucosa thickness, the depletion of Paneth cells, an inhibition of TJ proteins expression. Short‐term treatment of rats with BSB3 before running prevented these adverse effects. Culture‐based analysis of the gut microbiota revealed significant elevation of pathogenic microorganisms only in treadmill‐exercised rats pretreated with PBS. High‐throughput 16S rRNA gene sequencing also revealed an increase in pathobionts in this group. Preventive treatment of animals with BSB3 resulted in predominance of beneficial bacteria. Conclusions BSB3 prevents excessive exercise‐associated complications by beneficial modulation of the gut microbiota. Significance and Impact of the Study Our study shows a new application of beneficial bacteria for prevention the adverse effects of excessive exercise.

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“…The mouse gut microbiota develops from low diversity in suckling mice to the diversity level of the dams around the time of weaning 16 . Additionally, the microbiota of 4 weeks old mice was reported to cluster differently from the same mice aged 12 and 24 weeks 22 . Altogether, this underlines that the murine microbiota around the time of weaning cannot be considered stable.…”

Section: Discussionmentioning

confidence: 99%

Microbiota composition of simultaneously colonized mice housed under either a gnotobiotic isolator or individually ventilated cage regime

Lundberg

Bahl

Licht

et al. 2017

Sci Rep

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Germ-free rodents colonized with microbiotas of interest are used for host-microbiota investigations and for testing microbiota-targeted therapeutic candidates. Traditionally, isolators are used for housing such gnotobiotic rodents due to optimal protection from the environment, but research groups focused on the microbiome are increasingly combining or substituting isolator housing with individually ventilated cage (IVC) systems. We compared the effect of housing systems on the gut microbiota composition of germ-free mice colonized with a complex microbiota and housed in either multiple IVC cages in an IVC facility or in multiple open-top cages in an isolator during three generations and five months. No increase in bacterial diversity as assessed by 16S rRNA gene sequencing was observed in the IVC cages, despite not applying completely aseptic cage changes. The donor bacterial community was equally represented in both housing systems. Time-dependent clustering between generations was observed in both systems, but was strongest in the IVC cages. Different relative abundance of a Rikenellaceae genus contributed to separate clustering of the isolator and IVC communities. Our data suggest that complex microbiotas are protected in IVC systems, but challenges related to temporal dynamics should be addressed.

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The structural alteration of gut microbiota in low-birth-weight mice undergoing accelerated postnatal growth

Cited by 23 publications

References 67 publications

Differences in the Gut Microbiota Establishment and Metabolome Characteristics Between Low- and Normal-Birth-Weight Piglets During Early-Life

Differences in the Gut Microbiota Establishment and Metabolome Characteristics Between Low- and Normal-Birth-Weight Piglets During Early-Life

Prevention of excessive exercise‐induced adverse effects in rats withBacillus subtilisBSB3

Microbiota composition of simultaneously colonized mice housed under either a gnotobiotic isolator or individually ventilated cage regime

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