The Structural Resistance’s Anatomy of Sweet Potato Leaves to Fungal Pathogen Sphaceloma batatas

Samiyarsih, Siti; Juwarno, Juwarno; Muljowati, Juni Safitri

doi:10.15294/biosaintifika.v10i1.12116

Cited by 10 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The observed anatomical profiles (paradermal and transversal section) included cuticle thickness, epidermis thickness, mesophyll thickness, stomata size, stomata, palisade ratio, and trichomes density per 1 mm 2 area of the epidermis of leaves. The observation of leaf anatomical profile was performing on five months old of a plant (Samiyarsih et al 2018). The 5 th leaf from the shoot bud was taken and cut into a 1 cm 2 piece.…”

Section: Embedding For Leaf Anatomical Profilesmentioning

confidence: 99%

“…Anatomical structure can be used as instructions to the structural resistance of plants to pathogen attack. Various pathogens attack sweet potato plants, such as the Sphaceloma batatas fungus that causes scurvy disease (scab) (Samiyarsih et al 2018). Anatomical profile and genetic diversity are essential information during the selection of the desired character to get superior crops.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anatomical profile and genetic variability of sweet potato (Ipomoea batatas) cultivars in Banyumas, Central Java, based on RAPD markers

et al. 2020

View full text Add to dashboard Cite

Abstract. Samiyarsih S, Fitrianto N, Azizah E, Herawati W, Rochmatino. 2020. Anatomical profile and genetic variability of sweet potato (Ipomoea batatas) cultivars in Banyumas, Central Java, based on RAPD markers. Biodiversitas 21: 1755-1766. Indonesia has developed a food consumption diversification based on local sources. Sweet potato (Ipomoea batatas) is one of the tubers of carbohydrate sources in addition to wheat, rice, corn, potatoes, and cassava. It has a wide distribution, and high cultivars diversity produces a high variation on its morphology, anatomy, and genetic modification. The objectives of this research were to investigate the anatomical profile and genetic variability of eight sweet potatoes (Ipomoea batatas) cultivars found in Banyumas, Central Java, Indonesia. Anatomical profile was observed by the embedding method and subjected to analysis of variance. DNA amplification using Random Amplified Polymorphic DNA (RAPD) method with 10 primers, namely OPA-1, OPA-2, OPA-3, OPA-4, OPA-9, OPA-11, OPA-13, OPA-15, OPA-16, and OPA-18. The result of the anatomical study showed that eight sweet potato cultivars have significantly different (p<0.05) variation in leaf anatomical profile. RAPD primers produced 52 amplified fragments varying from 100 to 1300 bp in size, and 90.40% of the amplification bands were polymorphic. The genetic similarity level is ranged from 0.37 to 0.93. Two unspecific groups were forming at a coefficient of 55% from the dendrogram. The first group consisted of one cultivar, Cangkuang, and the second group consisted of seven cultivars Antin, Ungu Tua, Borobudur, Sukuh, Sari, Beta, and Kidal. The nearest relationships cultivars were Borobudur, and Ungu Tua had the highest similarity coefficient of 93%, and the lowest similarity found in Cangkuang and Antin with a coefficient of 37%. The range of genetic distance of eight cultivars was from 0.37-0.93. Implementation of the research would particularly useful for the identification and evaluation of the genetic improvement of sweet potato cultivars.

show abstract

Section: Embedding For Leaf Anatomical Profilesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anatomical profile and genetic variability of sweet potato (Ipomoea batatas) cultivars in Banyumas, Central Java, based on RAPD markers

et al. 2020

View full text Add to dashboard Cite

show abstract

“…M0F60). Hasil ini tidak sejalan dengan hasil penelitian Samiyarsih et al (2018) yang menunjukkan bahwa inokulasi jamur menyebab-kan penurunan panjang dan lebar stomata daun ubi jalar (Sphaceloma batatas). Infeksi jamur Peronosclerospora maydis pada tanaman jagung menyebabkan peningkatan nilai kerapatan stomata (Agustamia et al, 2016).…”

Section: Indeks Stomataunclassified

Produksi Cabai Merah (Capsicum annuum L.) dari Benih yang Diinduksi Medan Magnet 0,2 mT dan Diinfeksi Jamur Fusarium sp.

et al. 2020

View full text Add to dashboard Cite

As one of the horticultural plants that has important economic value, the cultivation of red chili (Capsicum annuum L.) is often constrained by the attack of Fusarium sp., which can cause losses due to decreased production. Induction of the magnetic field on the plant is known to be able to increase growth, development, and production in various plants. In this research, we studied the production of chili grown from seeds exposed to a 0.2 mT magnetic field and then infected with Fusarium sp. The study was conducted factorial using a completely randomized design with 2 factors. The first factor is the exposure of a 0.2 mT magnetic field to the seeds consisting of controls, without exposure to a magnetic field (M0), magnetic field exposure for 7 minutes 48 seconds (M7), and 15 minutes 30 seconds (M15). The second factor is Fusarium sp. infection on seeds that have been exposed to a magnetic field for 60 minutes by immersion of the seeds with spore suspension of Fusarium sp. with the density of 1 x 107 conidia/ml (F60), and without Fusarium sp. infection (F0). Each treatment unit was repeated 5 times. The data obtained were analyzed for variance at α = 5% followed by the least significant difference test using the Fishers Test at α = 5%. The results of data analysis showed that the interaction of 0,2 mT magnetic field exposure and infection of Fusarium sp. in chili seeds significantly affect the stomata index, the fruiting rate, and the number of fruit but do not affect the content of total chlorophyll, carbohydrate, as well as the number of flowers. The highest fruiting rate and number of fruits obtained from the treatment result of 0.2 mT magnetic field exposure for 15 minutes 36 seconds without Fusarium sp. infection (M15F0). Infection of Fusarium sp. in these seeds (M15F60) reduce the fruiting rate and the number of fruit but it is not significant and even almost similar with the fruiting rate and the fruit number of the plant growth from control treatment (M0F60). Key words: magnetic field, Fusariumsp., seed, and production.

show abstract

“…Observation of anatomical characters was performed using a binocular microscope, Olympus CH-20. Measurement of anatomical characters was done using a calibrated ocular micrometer (Hammed et al 2013;Pitoyo et al 2018;Samiyarsih et al 2018).…”

Section: Anatomical Charactersmentioning

confidence: 99%

Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents

2019

Self Cite

View full text Add to dashboard Cite

Abstract. Samiyarsih S, Naipospos N, Palupi D. 2019. Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents. Biodiversitas 20: 2986-2993. Catharanthus roseus (L.) is an important medicinal plant of the family Apocynaceae used to treat many of the diseases. C. roseus also possess good antioxidant potential. The purpose of this research was 1) to determine the variability of C. roseus based on the morphological, anatomical characters and chlorophyll contents, 2) to know the differences in morphological, anatomical characteristics and chlorophyll contents of C. roseus found in Banyumas Regency. The research used a survey method, with a purposive sampling technique. Preparation of leaf for anatomical analysis was based on paraffin method. The results showed that C. roseus in Banyumas Regency were grouped into eight varieties. The morphological characters observed consisted of 21 characters, 8 quantitative characters, and 13 qualitative characters. Most of the quantitative morphological and anatomical characters were significantly diverse (p <0.05) except for petiole length, corolla number, stomata width and number of trichomata. The eight varieties of C. roseus have different chlorophyll a, chlorophyll b, and total chlorophyll contents but their anthocyanin contents were not significantly different.

show abstract

The Structural Resistance’s Anatomy of Sweet Potato Leaves to Fungal Pathogen Sphaceloma batatas

Cited by 10 publications

References 8 publications

Anatomical profile and genetic variability of sweet potato (Ipomoea batatas) cultivars in Banyumas, Central Java, based on RAPD markers

Anatomical profile and genetic variability of sweet potato (Ipomoea batatas) cultivars in Banyumas, Central Java, based on RAPD markers

Produksi Cabai Merah (Capsicum annuum L.) dari Benih yang Diinduksi Medan Magnet 0,2 mT dan Diinfeksi Jamur Fusarium sp.

Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents

Contact Info

Product

Resources

About