The structure of a zeta class glutathione S-transferase from Arabidopsis thaliana: characterisation of a GST with novel active-site architecture and a putative role in tyrosine catabolism

Thom, Russell; Dixon, David P.; Edwards, Robert; Cole, David J.; Lapthorn, A.J.

doi:10.1006/jmbi.2001.4638

Cited by 117 publications

(100 citation statements)

References 54 publications

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“…GST is also capable of detoxifying ROS and its activity increases in cases of oxidative stress in plant cells (PFLUGMACHER et al 1999, THOM et al 2001. The observed increase in GST activity at Dona Paula site (Tab.…”

Section: Antioxidant Defence Systemsmentioning

confidence: 90%

Oxidative stress and antioxidant indices of the marine red alga Porphyra vietnamensis

Pise¹,

Gaikwad²,

Jagtap³

2013

Acta Botanica Croatica

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-Oxidative stress and antioxidant defence systems were assessed in a marine red alga Porphyra vietnamensis Tanaka et Pham-Hoang Ho, from India. Lipid peroxidation (LPX) and hydrogen peroxide (H 2 O 2 ) were measured as oxidative stress markers. Antioxidant defences were measured as catalase (CAT), glutathione S-transferase (GST) and ascorbic acid (AsA), in order to understand their dissimilarity with respect to environmental conditions (pollution levels) from selective locations along the central west coast of India. Levels of LPX, H 2 O 2 , CAT and GST were significantly higher in samples collected from Dona Paula than in samples from Malvan and Kunkeshwar, while a lower concentration of AsA was found in samples from Dona Paula. Heavy metals such as Cd, Pb and Hg in higher concentrations in these areas than in other sites were also observed. Variation of oxidative stress indices in response to the accumulation of heavy metals within P. vietnamensis could be used as molecular biomarkers for the assessment and monitoring of environmental quality in ecologically sensitive marine habitats.

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Section: Antioxidant Defence Systemsmentioning

confidence: 90%

Oxidative stress and antioxidant indices of the marine red alga Porphyra vietnamensis

Pise¹,

Gaikwad²,

Jagtap³

2013

Acta Botanica Croatica

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“…[10][11][12] In the present study, saturation mutagenesis was performed at three noncatalytic residues in and near the active site to determine whether DCA-DC activity would be enhanced by altering the structure of the active site without changing the catalytic mechanism. Overlap extension PCR was used to introduce multiple mutations, as these three residues are distant in the primary sequence.…”

Section: Discussionmentioning

confidence: 99%

“…It forms a homodimer with each subunit consisting of 221 amino acids. 10) Like typical GSTs, the GSH-binding site (G-site) and hydrophobic substrate-binding site (H-site) of AtGSTZ-1 are located in the N-and C-terminal domains respectively (Fig. 1A).…”

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confidence: 99%

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Evolution of the Catalytic Activity ofArabidopsis thalianaGlutathione Transferase Zeta Class-1 by Saturation Mutagenesis

Chen

Liu

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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“…Actually, human and fungal GSTZ proteins occur as maleylacetoacetate isomerase (MAAI) in phenylalanine and tyrosine metabolic pathways (Fernandez-Canon and Penalva 1998). Thom et al (2001) showed that the GSTZ protein in Arabidopsis exhibited a similar activity to that of MAAI. The function of MAAI is important because MAAI might be involved in the nitrogen metabolism in plants.…”

Section: Introductionmentioning

confidence: 99%

Disruption of GSTZ1 Gene by Large Genetic Alteration in Oryza glaberrima

Tsuchiya

Nakamura

2004

Breed. Sci.

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After the completion of the genome sequencing project of common rice (Oryza sativa L.), comparative genomic studies between rice and related species became important to reveal the function of each gene. The rice genome contains two copies of the gene encoding zeta class glutathione S-transferase (GSTZ) that is reported to be the enzyme in the catabolic pathway of tyrosine and phenylalanine. Two GSTZ genes of O. sativa, OsGSTZ1 and OsGSTZ2, display a tandem arrangement. Upstream OsGSTZ1 gene is constitutively expressed, whereas the downstream OsGSTZ2 gene is inducible by stresses. We analyzed the expression of the GSTZ gene in the African cultivated species O. glaberrima and wild species O. longistaminata by using RT-PCR. The results showed that both GSTZ1 and GSTZ2 genes were expressed in O. longistaminata, whereas only the cDNA fragment of downstream GSTZ2 was detected in O. glaberrima. Thus, the genomic sequence of the GSTZ1 locus of O. glaberrima was determined by PCR genomic walking. Sequence comparison between O. sativa and O. glaberrima revealed that the genomic sequence upstream from the eighth intron of the OgGSTZ1 gene was highly homologous, in inverted orientation, to a BAC clone, over 1-Mb apart from the OsGSTZ1 gene in O. sativa. This result suggested that the OgGSTZ1 gene was disrupted by a large rearrangement or inversion. The genetic alteration was also observed in several lines of O. glaberrima and its ancestral wild species O. barthii. The loss of the OgGSTZ1 gene in O. glaberrima is important, as the expression of the gene is 12-fold higher than that of OsGSTZ2 in O. sativa and since O. sativa and O. glaberrima differ in several such as seed yield and annual/perennial habitat that might be related to the translocation of nitrogen metabolites from leaves to seeds.

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The structure of a zeta class glutathione S-transferase from Arabidopsis thaliana: characterisation of a GST with novel active-site architecture and a putative role in tyrosine catabolism

Cited by 117 publications

References 54 publications

Oxidative stress and antioxidant indices of the marine red alga Porphyra vietnamensis

Oxidative stress and antioxidant indices of the marine red alga Porphyra vietnamensis

Evolution of the Catalytic Activity ofArabidopsis thalianaGlutathione Transferase Zeta Class-1 by Saturation Mutagenesis

Disruption of GSTZ1 Gene by Large Genetic Alteration in Oryza glaberrima

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