The structure of inducing factors for virginiamycin production in Streptomyces virginiae.

Yamada, Yasuhiro; K, Sugamura; Kondo, Koichi; Yanagimoto, Masakatsu; Okada, Hirosuke

doi:10.7164/antibiotics.40.496

Cited by 169 publications

(112 citation statements)

References 4 publications

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“…Specific regulatory factors of antibiotic production have also been reported in the literature. A-factor and virginiae butanolides, compounds with a gamma-butyrolactone structure, have been shown to be involved in the regulatory cascade leading to streptomycin and virginiamycin production respectively (Hara & Beppu, 1982 ;Yamada et al, 1987). Although the occurrence of similar compounds is known in ' S. pristinaespiralis ' cultures (Paquet et al, 1992), their possible role in the onset of pristinamycin production is not discussed in this paper.…”

Section: Possible Regulation Of Production Of Pristinamycinmentioning

confidence: 96%

Nitrogen source governs the patterns of growth and pristinamycin production in ‘Streptomyces pristinaespiralis’

Voelker¹,

Altaba²

2001

Microbiology

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Phosphate-limited synthetic culture media were designed to investigate the growth and the pristinamycin production of ' Streptomyces pristinaespiralis ' using different nitrogen sources. During balanced growth, either mineral or organic nitrogen sources were readily utilized. However, glutamate and alanine were used as both nitrogen and carbon source, sparing the utilization of the primary carbon source, glucose. Valine was utilized only for its nitrogen and consequently 2-ketoisovalerate was excreted in the medium. Ammonium prevented the utilization of nitrate. Upon phosphate limitation, glycerol, originating from the breakdown of teichoic acids, was released, allowing the recovery of phosphate from the cell wall and the continuation of growth. Under such conditions, ammonium was excreted following the consumption of glutamate and alanine and was later reassimilated after exhaustion of the primary nitrogen source. The mode of utilization of valine prevented the production of pristinamycins due to excretion of 2-ketoisovalerate, one of their direct precursors. For other nitrogen sources, pristinamycin production was controlled by nitrogen catabolic regulation linked to the residual level of ammonium. In the case of nitrate, the negative regulation was alleviated by the absence of ammonium and production then occurred precociously. In the case of amino acids and ammonium, production was delayed until after exhaustion of amino acids and depletion of ammonium.

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Section: Possible Regulation Of Production Of Pristinamycinmentioning

confidence: 96%

Nitrogen source governs the patterns of growth and pristinamycin production in ‘Streptomyces pristinaespiralis’

Voelker¹,

Altaba²

2001

Microbiology

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“…8 ; Kleiner et al, 1976). The later rediscovery of the Russian work, and its enormous development in S. griseus, Streptomyces virginiae and S. coelicolor, primarily by the groups of Teruhiko Beppu and Sueharu Horinouchi and of Yasuhiro Yamada (Hara & Beppu, 1982 ;Yamada et al, 1987), has been one of the most significant facets of Streptomyces genetics over recent years (e.g. Onaka et al, 1998).…”

Section: The In Vivo Yearsmentioning

confidence: 99%

Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico

1999

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“…4), BarA from Streptomyces virginiae (Okamoto et al, 1995) and A-factor receptor from Streptomyces griseus (Onaka et al, 1995). A-factor is a bacterial pheromone widely employed to trigger morphological development or antibiotic biosynthesis in Streptomyces, which is thought to elicit these effects through interactions with A-factor-binding proteins (Hara & Beppu, 1982;Yamada et al, 1987;Horinouchi & Beppu, 1990). Butyrolactone receptors are a subgroup of the tetR family of regulatory proteins which have a common a-helix-turn-a-helix (HTH) DNAbinding motif for switching off the expression of key genes.…”

Section: Msssgdarpsqkgillpaarndtdeaagrrsiawpvartcpfsppeqyaalrae-epia mentioning

confidence: 99%

The tylosin biosynthetic cluster from Streptomyces fradiae: genetic organization of the left region

Fouces¹,

Mellado²,

Dı́ez³

et al. 1999

Microbiology

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The genetic organization of the left edge (ty/€DHFJ region) of the tylosin biosynthetic gene cluster from Streptomyces fradiae has been determined. Sequence analysis of a 12.9 kb region has revealed the presence of 11 ORFs, 10 of them belonging to the biosynthetic cluster. The putative functions of the proteins encoded by these genes are as follows: peptidase (ORF1, d d d ) , tylosin resistance determinant (ORFZ, tlrB), glycosyltransferase (ORF3, ty//U), methyltransferase (ORF4, tyl€), ketoreductase (ORF5, tylD), ferredoxin (ORF6, tylH2), cytochrome P450 (ORF7, tylH7), methyltransferase (ORF8, tylF), epimerase (ORF9, tylJ), acyl-CoA oxidase (ORF10, tylP) and receptor of regulatory factors (ORF11, tylQ). The functional identification of the genes in the proposed tylosin biosynthetic pathway has been deduced by database searches and previous genetic complementation studies performed with tylosin idiotrophic mutants blocked a t various stages in tylosin biosynthesis. The tlrB gene has been shown to be useful as a tylosin resistance marker in Streptomyces liwidans, Streptomyces parvulus and Streptomyces coelicolor and the effect of tylF on macrocin depletion has been confirmed. A pathway for the biosynthesis of 6-deoxy-~-allose, the unmethylated mycinose precursor, involving the genes tylD, tylJ and tylN is proposed.

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The structure of inducing factors for virginiamycin production in Streptomyces virginiae.

Cited by 169 publications

References 4 publications

Nitrogen source governs the patterns of growth and pristinamycin production in ‘Streptomyces pristinaespiralis’

Nitrogen source governs the patterns of growth and pristinamycin production in ‘Streptomyces pristinaespiralis’

Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico

The tylosin biosynthetic cluster from Streptomyces fradiae: genetic organization of the left region

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