The structure of myxoxanthophyll

Hertzberg, Sissel; Liaaen‐Jensen, Synnøve

doi:10.1016/s0031-9422(00)85566-1

Cited by 67 publications

(20 citation statements)

References 34 publications

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“…4A). This type of fragmentation had been previously observed [8,18]. By the same token, the two ion peaks m/z 742 and m/z 509 detected in the spectrum of the deoxymyxoxanthophyll spectrum (Fig.…”

Section: Nature Of the Myxoxanthophyll-like Compound In The Acrtr Strainsupporting

confidence: 81%

“…PCC 6803 myxoxanthophyll is 28 mass units heavier than the compound isolated from O. rubrescens [8]. As the absorption spectrum of myxoxanthophyll in Synechocystis' is in agreement with the one that was previously published [8], the carotene backbone must be the same; however, the sugar moiety of this carotenoid glycoside may not be rhamnose. Instead, the apparent increase in molecular weight by 28 mass units suggests that the sugar moiety of the Synechoo'stis sp.…”

Section: Nature Of the Myxoxanthophyll-like Compound In The Acrtr Strainsupporting

confidence: 74%

“…As the crtR gene codes for a Table 1 Carotenoid content in the wild-type and the AcrtR strains of Synechocystis sp. Comparison between UV-visible absorption spectra of myxoxanthophyll (dashed line) [8], and the myxoxanthophyll-like compound from the AcrtR strain (solid line), recorded with a photodiode array detector on the HPLC. m, myxoxanthophyll; z, zeaxanthin; chl, chlorophyll a; e, echinenone: t-car, t-carotene: and x. the myxoxanthophyll-like compound that is specific for the zXcrtR strain.…”

Section: Nature Of the Myxoxanthophyll-like Compound In The Acrtr Strainmentioning

confidence: 99%

“…The molecular mass of myxoxanthophyll (C46H6607) was expected to be 730 based on the determination of the structure of myxoxanthophyll isolated from O. rubrescens [8]. However, myxoxanthophyll isolated from Synechocystis sp.…”

Section: Nature Of the Myxoxanthophyll-like Compound In The Acrtr Strainmentioning

confidence: 99%

“…It was first isolated from the cyanobacterium Oscillatoria rubrescens [7]. Its structure was determined as a mixed carotenoid glycoside in which rhamnose is the dominant sugar moiety (90%) and a hexose is a minor component [8]. Although most of the genes involved in carotenoid biosynthesis have been identified in several organisms, including Synechocystis sp.…”

Section: Introductionmentioning

confidence: 99%

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The zeaxanthin biosynthesis enzyme ²‐carotene hydroxylase is involvedin myxoxanthophyll synthesis in Synechocystis sp. PCC 6803

Lagarde

Vermaas

1999

FEBS Letters

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Section: Nature Of the Myxoxanthophyll-like Compound In The Acrtr Strainsupporting

confidence: 81%

Section: Nature Of the Myxoxanthophyll-like Compound In The Acrtr Strainsupporting

confidence: 74%

Section: Nature Of the Myxoxanthophyll-like Compound In The Acrtr Strainmentioning

confidence: 99%

Section: Nature Of the Myxoxanthophyll-like Compound In The Acrtr Strainmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The zeaxanthin biosynthesis enzyme ²‐carotene hydroxylase is involvedin myxoxanthophyll synthesis in Synechocystis sp. PCC 6803

Lagarde

Vermaas

1999

FEBS Letters

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Characterization via liquid chromatography coupled to diode array detector and tandem mass spectrometry of supercritical fluid antioxidant extracts of Spirulina platensis microalga

Mendiola

Marín

Hernández³

et al. 2005

J of Separation Science

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Spirulina platensis microalga has been extracted on a pilot scale plant using supercritical fluid extraction (SFE) under various extraction conditions. The extraction yield and the antioxidant activity of the extracts were evaluated in order to select those extracts with both the highest antioxidant capacity and a good extraction yield. These extracts were characterized using LC coupled to diode array detection (DAD) and LC coupled to mass spectrometry (MS) with two different interfaces, atmospheric pressure chemical ionization (APCI) and electrospray (ESI) which allowed us to perform tandem MS by using an ion trap analyzer. The best extraction conditions were as follows: CO2 with 10% of modifier (ethanol) as extraction solvent, 55 degrees C (extraction temperature) and 220 bar (extraction pressure). Fractionation was achieved by cascade depressurization providing two extracts with different activity and chemical composition. Several compounds have been identified in the extracts, corresponding to different carotenoids previously identified in Spirulina platensis microalga along with chlorophyll a and some degradation products. Also, the structure of some phenolic compounds could be tentatively identified. The antioxidant activity of the extracts could be attributed to some of the above mentioned compounds.

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Separation and characterization of antioxidants from Spirulina platensis microalga combining pressurized liquid extraction, TLC, and HPLC‐DAD

Jaime

Mendiola

Herrero

et al. 2005

J of Separation Science

122

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A new procedure has been developed to separate and characterize antioxidant compounds from Spirulina platensis microalga based on the combination of pressurized liquid extraction (PLE) and different chromatographic procedures, such as TLC, at preparative scale, and HPLC with a diode array detector (DAD). Different solvents were tested for PLE extraction of antioxidants from S. platensis microalga. An optimized PLE process using ethanol (generally recognized as safe, GRAS) as extraction solvent has been obtained that provides natural extracts with high yields and good antioxidant properties. TLC analysis of this ethanolic extract obtained at 115 degrees C for 15 min was carried out and the silica layer was stained with a DPPH (diphenyl-pycril-hydrazyl) radical solution to determine the antioxidant activity of different chromatographic bands. Next, these colored bands were collected for their subsequent analysis by HPLC-DAD, revealing that the compounds with the most important antioxidant activity present in Spirulina extracts were carotenoids, as well as phenolic compounds and degradation products of chlorophylls.

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The structure of myxoxanthophyll

Cited by 67 publications

References 34 publications

The zeaxanthin biosynthesis enzyme ²‐carotene hydroxylase is involvedin myxoxanthophyll synthesis in Synechocystis sp. PCC 6803

The zeaxanthin biosynthesis enzyme ²‐carotene hydroxylase is involvedin myxoxanthophyll synthesis in Synechocystis sp. PCC 6803

Characterization via liquid chromatography coupled to diode array detector and tandem mass spectrometry of supercritical fluid antioxidant extracts of Spirulina platensis microalga

Separation and characterization of antioxidants from Spirulina platensis microalga combining pressurized liquid extraction, TLC, and HPLC‐DAD

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