The Structure of the Lipid A‐Core Region of the Lipopolysaccharides from <i>Vibrio cholerae</i> O1 Smooth Strain 569B (Inaba) and Rough Mutant Strain 95R (Ogawa)

Vinogradov, Evgeny; Bock, Klaus; Holst, Otto; Brade, Helmut

doi:10.1111/j.1432-1033.1995.152_1.x

Cited by 41 publications

(43 citation statements)

References 19 publications

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“…Electrospray mass spectrometry of pool I gave a molecular ion at m\z l 1650, consistent with the structure proposed for the core of V. cholerae O1 LPS (Vinogradov et al, 1995) (Fig. 5).…”

Section: Characterization Of Lpss and Derived Fragmentssupporting

confidence: 50%

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Immunochemical characterization of an Ogawa-Inaba common antigenic determinant of Vibrio cholerae O1

et al. 1999

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Cholera remains an important public health problem in many parts of the world and the availability of an effective cholera vaccine is important for the prevention of cholera in the countries affected by this disease. Despite the apearance in 1992 of a new serogroup, O139, of Vibrio cholerae, most of the cholera outbreaks are still caused by V. cholerae O1 biotype El Tor. Vaccine trials in Asia from 1968 to 1971, and studies of the production of serotypespecific antiserum in rabbits and of the protective activity of monoclonal antibodies against diarrhoeal disease in neonatal mice, have led to the conclusion that the Ogawa serotype contains a specific antigenic determinant whereas the Inaba serotype contains a different antigenic determinant that cross-reacts with the Ogawa serotype. By studying the binding of anti-Ogawa monoclonal antibodies to synthetic oligosaccharide fragments mimicking the Ogawa O-specific polysaccharide, it has been shown that the terminal monosaccharide, bearing the 2-O-methyl group in the O-specific polysaccharide, is most probably the serotype-specific determinant for the Ogawa strain. However, study of the binding of a monoclonal antibody recognizing both Ogawa and Inaba serotypes suggested partial recognition of the core as well as of the O-specific polysaccharide of the LPS of V. cholerae O1. To further characterize this antigenic determinant that is common to the Ogawa and Inaba serotypes, the core and the O-specific polysaccharide linked to the core of V. cholerae O1 LPS were purified by preparative electrophoresis. The O-specific polysaccharide linked to the core was subjected to periodate oxidation to destroy sugars from the core. Binding studies of these purified saccharide fragments to a monoclonal antibody which is protective in mice and specific to the antigenic determinant common to Ogawa and Inaba serotypes showed that both the core and the O-specific polysaccharide are involved in this common antigenic determinant. This explains how the presence or the absence of the Ogawa-specific antigenic determinant would lead to the expression of two independent antigenic determinants of V. cholerae O1, one specific to the Ogawa serotype and the other common to both Ogawa and Inaba serotypes.

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“…Electrospray mass spectrometry of pool I gave a molecular ion at m\z l 1650, consistent with the structure proposed for the core of V. cholerae O1 LPS (Vinogradov et al, 1995) (Fig. 5).…”

Section: Characterization Of Lpss and Derived Fragmentssupporting

confidence: 50%

“…α--mannopyranosyl residues] is resistant (Kenne et al, 1982 ;Vinogradov et al, 1995 ;Wang et al, 1998).…”

Section: Methodsmentioning

confidence: 99%

Immunochemical characterization of an Ogawa-Inaba common antigenic determinant of Vibrio cholerae O1

et al. 1999

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“…LPS was purified by aqueous phenol extraction and ultracentrifugation from killed cells, as described by Vinogradov et al (1995), with modifications. The bacteria were scraped off BHIBC agar into PBS, and following phenol extraction, 4 vols distilled water was added and the mixture dialysed with tap water until no phenol odour remained.…”

Section: Methodsmentioning

confidence: 99%

Attenuation and protective efficacy of an O-antigen-deficient mutant of Francisella tularensis LVS

Ryder

Mandal

et al. 2007

Microbiology

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Francisella tularensis is a zoonotic, Gram-negative coccobacillus that causes tularemia in humans and animals. F. tularensis subspecies tularensis (type A) and F. tularensis subspecies holarctica (type B) are antigenically similar and more virulent than Francisella novicida in humans. The genetic locus that encodes the LPS O antigen was found to be substantially different between the type B live vaccine strain (LVS) and F. novicida. One LVS-specific gene with homology to a galactosyl transferase was selected for allelic replacement using a sacB-chloramphenicol expression suicide plasmid, and recombinants were screened for colony morphology on Congo red agar that matched that of F. novicida. Two mutants (WbtI S187Y and WbtI G191V ) were isolated that contained substitutions in conserved motifs in the sugar transamine/perosamine synthetase (WbtI) of the O-antigen locus, and the latter mutant was extensively tested and characterized. WbtI G191V grew at the same rate as the parent strain in Chamberlain's defined medium, completely lacked O antigen, was serum-sensitive but could grow in a mouse macrophage cell line, had increased resistance to sodium deoxycholate, and was highly attenuated in mice. Complementation of WbtI G191V with the wild-type wbtI gene in trans restored normal LPS synthesis, phenotypic properties similar to the parent, and virulence in mice. Immunization with WbtI G191V protected mice against a relatively low-dose intraperitoneal challenge with LVS, but was less protective against a high-dose challenge. These results indicate that complete loss of O antigen alters the surface phenotype and abrogates virulence in F. tularensis, but also compromises the induction of full protective immunity against F. tularensis infection in mice.

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“…1A and B). For the core OS of serogroups O1, O139, and O22 it was found that one D-fructose is linked to the D-glucose residue on HepI (15,16,37,38,68), while in the H11 isolate D-sedoheptulose is found instead at the same position (7,68). Other basic differences between the O1, O139, and O22 core OS and the H11 core OS are the presence of a terminal hepto-FIG.…”

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confidence: 99%

Comparative and Genetic Analyses of the Putative Vibrio cholerae Lipopolysaccharide Core Oligosaccharide Biosynthesis ( wav ) Gene Cluster

et al. 2002

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We identified five different putative wav gene cluster types, which are responsible for the synthesis of the core oligosaccharide (OS) region of Vibrio cholerae lipopolysaccharide. Preliminary evidence that the genes encoded by this cluster are involved in core OS biosynthesis came from analysis of the recently released O1 El Tor V. cholerae genome sequence and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of O1 El Tor mutant strains defective in three genes (waaF, waaL, and wavB). Investigations of 38 different V. cholerae strains by Southern blotting, PCR, and sequencing analyses showed that the O1 El Tor wav gene cluster type is prevalent among clinical isolates of different serogroups associated with cholera and environmental O1 strains. In contrast, we found differences in the wav gene contents of 19 unrelated non-O1, non-O139 environmental and human isolates not associated with cholera. These strains contained four new wav gene cluster types that differ from each other in distinct gene loci, providing evidence for horizontal transfer of wav genes and for limited structural diversity of the core OS among V. cholerae isolates. Our results show genetic diversity in the core OS biosynthesis gene cluster and predominance of the type 1 wav gene locus in strains associated with clinical cholera, suggesting that a specific core OS structure could contribute to V. cholerae virulence.Vibrio cholerae is a genetically diverse species that persists in aquatic ecosystems and is often associated with plankton and other aquatic organisms (13). V. cholerae is classified on the basis of biochemical tests and DNA homology studies and is further subdivided into serogroups based on the antigenicity of surface polysaccharides (20). Today more than 193 serogroups are known (72). The ability to cause pandemic cholera is mainly restricted to the nonencapsulated serogroup O1, which is further subdivided mainly into two serotypes (Inaba and Ogawa) and biotypes (classical and El Tor). However, during 1992 and 1993, cholera-like outbreaks in Asia were caused by strains of serogroup O139. Molecular and epidemiological analyses, as well as phage typing, revealed that O139 strains are highly related to the O1 El Tor strains. Hence, it is assumed that the epidemic O139 strains were derived from O1 El Tor strains (for a review see reference 20), differing specifically in the genes encoding for the synthesis of a novel type of cell surface polysaccharide (71). In particular, it was found that the genes encoding the O1 antigen had been replaced by a capsule gene locus carrying the genes involved in the synthesis and transport of the O139 antigen and the O139 capsule (reviewed in reference 64). It was also determined that the structures of the O139 antigen and the O139 capsule were identical (36, 38).There is good evidence that pandemic V. cholerae O1 strains have become adapted to the human intestine by acquisition of virulence factors. Two known factors are cholera toxin (CT), encoded by the filamentous phage CTX⌽, and ...

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The Structure of the Lipid A‐Core Region of the Lipopolysaccharides from Vibrio cholerae O1 Smooth Strain 569B (Inaba) and Rough Mutant Strain 95R (Ogawa)

Cited by 41 publications

References 19 publications

Immunochemical characterization of an Ogawa-Inaba common antigenic determinant of Vibrio cholerae O1

Immunochemical characterization of an Ogawa-Inaba common antigenic determinant of Vibrio cholerae O1

Attenuation and protective efficacy of an O-antigen-deficient mutant of Francisella tularensis LVS

Comparative and Genetic Analyses of the Putative Vibrio cholerae Lipopolysaccharide Core Oligosaccharide Biosynthesis ( wav ) Gene Cluster

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