The study of DNA adduct 8-hydroxy-2′deoxyguanosine (8-OHdG) formation of butylated hydroxyanisole (BHA) and its metabolite ter-butyl hydroquinone (TBHQ) through in vitro reaction with Calf Thymus DNA and 2′deoxyguanosine

Abstract

View the article online for updates and enhancements. Related contentStudy of DNA adduct 8 hydroxy-2'-deoxyguanosine (8-OHdG) formation through fenton reaction with tertbutylhydroquinone (TBHQ) and butyl hydroxy toluene (BHT) S Handayani, I C Dani, Budiawan et al.-DNA interaction studies of new nano metal based anticancer agent: validation byspectroscopic methods Sartaj Tabassum, Girish Chandra Sharma, Farukh Arjmand et al. Although WHO declared their safety, the use of these preservatives are still controversial because some studies showed that BHA induced proliferative effects in animal testing and TBHQ is considered as carcinogenic and causes DNA cleavage. This study is aimed to analyze the interaction between Calf Thymus DNA with BHA and TBHQ which are mediated with Copper (II) Chloride. The result of the study in spectrophotometric showed there was bathochromic shift as much as 2-3 nm in DNA treated with TBHQ. The next analysis used HPLC method in stationary phase of ODS, mobile phase of 10mM Natrium Hydrogen Phosphate Buffer and Methanol (85 : 15) for DNA adduct formation, 8-Hydroxy-2-Deoxyguanosine (8-OHDG) as biomarker of risk cancer. The resultof the study showed the formation of DNA adduct 8-OHDG in the interaction between DNA and 20-500 ppm of TBHQ. The 8-OHdG formation was greatly increased by the higher concentration of TBHQ. The relative amount of 8 OHDG which formed was reached 946/10 5 deoxyguanosine in DNA bases. Confirmation test by LCMS/MS was characterized with the detection of mother ion peak (m/z 284); fragment ion peaks at m/z 167.9, and 139.9; at retention time 3.52 min. Meanwhile the interaction between DNA and 50-250 ppm BHA did not induce 8-OHDG.

Solid Phase Oligo-DNA Extraction from Complex Medium Using an Aminated Graphene/Nitrocellulose Membrane Hybrid

“…The phosphate groups on the ssDNA backbone and the NH 2 groups on the membrane surface are both partially protonated, resulting in a reduced net charge on both molecules. This reduction in charge weakens the electrostatic interactions between ssDNA and the membrane, making it easier for ssDNA to detach or desorb from the membrane surface [ 68 , 69 , 70 , 71 ].…”