The Study of Necrogenic Action of 0,2% Noradrenaline Hydrotartrate Solution During Its Intraprostatic Injection in Rats

Abstract

Abstract. Background. Previously, we first formulated the concept of targeted prostatic pharmaconecrosis (TPPhN). TPPhN is the death of those areas of the prostate tissue, that are proven or suspected to contain tumor cells of prostate cancer, due to the direct injection of a pharmacologically active substance into the prostate tissue. The aim: to study whether TPPhN occurs in the prostate tissue of male rats three hours after injection of 0.05 ml noradrenaline hydrotartrate (NH) solution into it. Materials and methods. The experiments were carried out on 12 outbred male rats aged 10 months. The animals were divided into two groups of six individuals each. Under anesthesia, the rats of the first group received only the injection of 0.05 ml 0,2% solution of NH into the left lobe of the prostate. Rats of the second group first received doxazosin 0.00025 g intragastrically, and then an intraprostatic injection of 0.05 ml of a 0.2 % solution of NH into the left lobe of the prostate was made. 3 hours after intraprostatic injection, macroscopic changes in the left lobe of the prostate were assessed, then the left lobe was resected. Results and conclusions. In all experiments, a demarcation line was clearly visible, delimiting the focus of TPPhN from intact tissue of the prostate. Typical morphological changes in the prostate tissue in the focus of TPPhN were studied. Close attention was paid to the characteristics of the ischemic penumbra between the focus of TPPhN and intact prostate tissue. It was found that in animals of the second group, the penumbra turned out to be clearer and narrower; due to this, the focus of TPPhN was sufficiently sharply delimited from normal prostate tissue. The fact established by us allows us to consider such a combination of substances as promising in the development of the TPPhN technique in humans.