The study of post-irradiation recovery of yeast: the ‘opremolecular period’

Korogodin, V.I.

doi:10.1016/0027-5107(93)90126-z

Cited by 16 publications

(9 citation statements)

References 12 publications

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“… Patrick et al (1964) reported that the post-treatment increase in viability following delayed plating was not attributable to cell multiplication but rather to true recovery as the intracellular repair of the treatment-induced damage. The effect was referred to as LH recovery and it was also supported by fluctuation test analyses ( Korogodin, 1993 ). Curiously, LH recovery in yeast was found to be specific for diploids ( Patrick et al, 1964 ) and variously reported as dependent on Rad51 and Rad52 ( Petin and Kabakova, 1981 ) or independent of Rad52 ( Moore et al, 2000 ).…”

Section: Discussionmentioning

confidence: 84%

Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations

et al. 2018

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After heavy exposure of Ustilago maydis cells to clastogens, a great increase in viability was observed if the treated cells were kept under starvation conditions. This restitution of viability is based on cell multiplication at the expense of the intracellular compounds freed from the damaged cells. Analysis of the effect of the leaked material on the growth of undamaged cells revealed opposing biological activity, indicating that U. maydis must possess cellular mechanisms involved not only in reabsorption of the released compounds from external environment but also in contending with their treatment-induced toxicity. From a screen for mutants defective in the restitution of viability, we identified four genes (adr1, did4, kel1, and tbp1) that contribute to the process. The mutants in did4, kel1, and tbp1 exhibited sensitivity to different genotoxic agents implying that the gene products are in some overlapping fashion involved in the protection of genome integrity. The genetic determinants identified by our analysis have already been known to play roles in growth regulation, protein turnover, cytoskeleton structure, and transcription. We discuss ecological and evolutionary implications of these results.

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Section: Discussionmentioning

confidence: 84%

Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations

et al. 2018

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“…1 and 2, respectively, e is the basis of the natural logarithim and β is the recovery constant, In other words, the recovery constnat is the probabiligy of reverovey per unit time and numerically it determines a fraction of the UV damage recovering per unit time. This model was used bedore to fit the recovery kinetics of yeast cells exposed to ionizing radiation applied alone (18,19) or combined with heat(21). It can be easilgy shown that Designating the right part of this equation through A( t ), We find that In bioligical terms, A( t ) reflects the relative part of the damage that the cell is able to recover from, but that has not yet been repaired after t hours of recovery.…”

Section: Resultsmentioning

confidence: 99%

“…Because the highest synergistic interaction of any agent is observed for their simultaneous application, in this article we analyzed the survival and the LHR of yeast cells after a simultaneous treatment with UV light and heat. Yeast cells are also convenient because a quantitative approach for the LHR in yeast cells has been proposed (18,19). The approach allows estimation of the probability of recovery per unit time and of the fraction of the irreversible damage.…”

Section: Introductionmentioning

confidence: 99%

Survival and Recovery of Yeast Cells After Simultaneous Treatment of UV Light Radiation and Heat^¶

Kim

Петин

Tkhabisimova

2004

Photochem & Photobiology

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Cell survival, synergistic interaction, liquid‐holding recovery (LHR) kinetics and inactivation forms after the simultaneous treatment with UV light (254 nm) and various high temperatures were studied in diploid yeast cells Saccharomyces cerevisiae. The synergistic interaction was observed within a certain temperature range in which there was a temperature that maximizes the synergistic effect. The LHR study revealed that both the extent and the rate of recovery greatly decreased with the increase in exposure temperature. A quantitative approach describing the LHR process as a decrease in the effective radiation dose was used to estimate the probability of recovery per unit time and the irreversible component of damage. Using the experimental data obtained and the mathematical model described, it was shwon that the irreversible component, i.e. the fraction of cells incapable of recovery, increased with the exposure temperature, whereas the recovery constant, i.e. the probability of recovery per unit time, was independent of the exposure temperature. The increase in the irreversible component was accompanied by an increase in cell death without postirradiation division. It is concluded based on this that the synergistic interaction of UV light radiation and hyperthermia in yeast cells is not related to the impairment of the recovery process itself and that it may be attributed to an increased yield of the irreversible damage.

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“…In other words, the recovery constant is the probability of recovery per unit time and numerically it determines a &action of the UV damage recovering per unit time. This model was used before to fit the recovery kinetics of yeast cells exposed to ionizing radiation applied alone (18,19) or combined with heat (21). It can be easily shown that…”

Section: Recovery Parametersmentioning

confidence: 99%

Survival and Recovery of Yeast Cells After Simultaneous Treatment of UV Light Radiation and Heat¶

Kim

Петин

Tkhabisimova

2004

Photochem Photobiol

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Cell survival, synergistic interaction, liquid-holding recovery (LHR) kinetics and inactivation forms after the simultaneous treatment with UV light (254 nm) and various high temperatures were studied in diploid yeast cells Saccharomyces cerevisiae. The synergistic interaction was observed within a certain temperature range in which there was a temperature that maximizes the synergistic effect. The LHR study revealed that both the extent and the rate of recovery greatly decreased with the increase in exposure temperature. A quantitative approach describing the LHR process as a decrease in the effective radiation dose was used to estimate the probability of recovery per unit time and the irreversible component of damage. Using the experimental data obtained and the mathematical model described, it was shown that the irreversible component, i.e. the fraction of cells incapable of recovery, increased with the exposure temperature, whereas the recovery constant, i.e. the probability of recovery per unit time, was independent of the exposure temperature. The increase in the irreversible component was accompanied by an increase in cell death without postirradiation division. It is concluded based on this that the synergistic interaction of UV light radiation and hyperthermia in yeast cells is not related to the impairment of the recovery process itself and that it may be attributed to an increased yield of the irreversible damage.

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The study of post-irradiation recovery of yeast: the ‘opremolecular period’

Cited by 16 publications

References 12 publications

Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations

Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations

Survival and Recovery of Yeast Cells After Simultaneous Treatment of UV Light Radiation and Heat^¶

Survival and Recovery of Yeast Cells After Simultaneous Treatment of UV Light Radiation and Heat¶

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