The study of probiotic properties of Lactobacillus sakei

Nikiforova, A. P.; Khazagaeva, Sofia; Khamagaeva, I. S.

doi:10.1063/5.0069078

Cited by 1 publication

(1 citation statement)

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“…Fung et al 50 achieved maximum growth of L. acidophilus FTCC 0291 in a soy whey medium supplemented with a combination of 7.25% (w/v) meat extract, 4.7% (w/v) vegetable extract and 6.85% (w/v) peptone. As L. sakei is one of the LAB species with more fastidious nutritional requirements 51 , it is expected that its development requires a wide variety of nutrients. Additionally, the use of the nitrogenous combination enables the replacement of other costly substrates present in the traditional MRS culture medium, such as beef extract, comprising a food-grade mixture.…”

Section: Mixture Designmentioning

confidence: 99%

Alternative culture medium design for biomass production of autochthonous meat starter Latilactobacillus sakei sp. sakei ACU-2

Galante

Prpich

Campos

et al. 2023

Sci Rep

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The autochthonous strain Latilactobacillus sakei sp. sakei ACU-2 was selected as a meat starter culture for dry sausage production. Transferring this strain from laboratory scale to industry requires an increase in biomass production, while lowering process costs. In this study, a combination of techniques was applied in order to optimize the culture medium composition to enhance biomass production of L. sakei ACU-2. One variable at a time experiments, Plackett–Burman design, and mixture design were performed to fulfill the strain nutritional requirements. Eventually, the optimized formulation contained 19.46 g/L yeast extract; 8.28 g/L whey protein concentrate; 2.26 g/L soy peptone; 30 g/L cerelose; 1 g/L Tween 80; 5 g/L sodium acetate; 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. When L. sakei ACU-2 was cultivated in a bioreactor using the alternative medium, an enhancement of 75.5% of biomass production was achieved, in comparison to its growth in the commercial de Man, Rogosa, and Sharpe medium. Furthermore, a reduction of 62–86% of the cost was also attained. These results support a promising large-scale application of the designed medium for high biomass yields of the starter culture at minor costs.

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Section: Mixture Designmentioning

confidence: 99%