The study of the antimicrobial activity of colloidal solutions of silver nanoparticles prepared using food stabilizers

Баландин, Глеб Владленович; Suvorov, O. A.; Shaburova, L. N.; Podkopaev, D. O.; Frolova, Yuliya; Ермолаева, Г А

doi:10.1007/s13197-014-1455-y

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Then, 3 wells of 7 mm diameter were made, distributed equidistantly in the Petri dish, and 20 µL of the AgNPs or EF was added. The plates were then incubated for 24 h at 37 • C; then, the inhibition zone was measured around the well using a vernier caliper [59].…”

Section: Antibacterial Activitymentioning

confidence: 99%

Optimization of Synthesis of Silver Nanoparticles Conjugated with Lepechinia meyenii (Salvia) Using Plackett-Burman Design and Response Surface Methodology—Preliminary Antibacterial Activity

et al. 2022

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In the present investigation, an ethanolic fraction (EF) of Lepechinia meyenii (salvia) was prepared and fractionated by gradient column chromatography, and the main secondary metabolites present in the EF were identified by HPLC-MS. Silver nanoparticles (AgNPs) were synthesized and conjugated with the EF of Lepechinia meyenii (salvia). The AgNPs synthesis was optimized using Plackett-Burman design and response surface methodology (RSM), considering the following independent variables: stirring speed, synthesis pH, synthesis time, synthesis temperature and EF volume. The AgNPs synthesized under the optimized conditions were characterized by UV visible spectroscopy (UV-VIS), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS) and Scanning Transmission Electron Microscopy (STEM). The antibacterial activity of the AgNPs against Staphylococcus aureus (ATCC® 25923) was evaluated. The following flavonoids were identified: rosmarinic acid, diosmin and hesperetin-7-O-rutinoside. The optimized conditions for the synthesis of nanoparticles were pH 9.45, temperature 49.8 °C, volume of ethanolic fraction 152.6 µL and a reaction time of 213.2 min. The obtained AgNPs exhibited an average size of 43.71 nm and a resonance plasmon of 410–420 nm. Using FT-IR spectroscopy, the disappearance of the peaks between 626.50 and 1379.54 cm−1 was evident with the AgNPs, which would indicate the participation of these functional groups in the synthesis and protection of the nanoparticles. A hydrodynamic size of 47.6 nm was obtained by DLS, while a size of 40–60 nm was determined by STEM. The synthesized AgNPs conjugated with the EF showed a higher antibacterial activity than the EF alone. These results demonstrate that the AgNPs synthesized under optimized conditions conjugated with the EF of the Lepechinia meyenii (salvia) presented an increased antibacterial activity.

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Section: Antibacterial Activitymentioning

confidence: 99%

Optimization of Synthesis of Silver Nanoparticles Conjugated with Lepechinia meyenii (Salvia) Using Plackett-Burman Design and Response Surface Methodology—Preliminary Antibacterial Activity

et al. 2022

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In vitro antimicrobial activity of colloidal nano silver

Popova

Ignatov

2023

BJVM

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The antimicrobial effect of colloidal nanosilver (AgNPs) at concentrations of 20 and 30 ppm against reference Esherichia coli, Salmonella enterica, Staphylococcus aureus, Clostridium perfringens, Candida albicans strains and two clinical isolates (Pseudominas aeruginosa and Streptococcus pyogenes) was tested. The classical agar diffusion method, determination of the minimum inhibitory concentrations (MIC) and the time of antimicrobial action of AgNPs were used. In the studies performed by the agar diffusion method, a very good inhibitory effect of AgNPs 30 ppm and to a much lesser extent of AgNPs 20 ppm, was reported against all studied microorganisms. The studied Gram-negative bacteria showed higher sensitivity to both preparations compared to the Gram-positive microorganisms (P>0.05). The lowest sensitivity was reported for S. aureus and C. albicans, and the highest - for P. aeruginosa and S. pyogenes. The lowest MICs of AgNPs 20 ppm were reported for P. aeruginosa and C. perfringens. For them, the MIC50 was 1 μg/mL, and for the rest - 2 μg/mL. Again, C. perfringens showed the highest sensitivity to AgNPs 30 ppm with MIC50 0.5 μg/mL, and the lowest one was that of S. aureus with MIC50 2 μg/mL. For the Gram-positive bacteria MIC values were higher than for Gram-negative ones. AgNPs 20 ppm and AgNPs 30 ppm inactivated all bacterial strains tested at final concentrations of 103 cells/mL and 104 cells/mL within 5 min; only C. albicans persisted longer. The tested microorganisms remained viable for significantly longer time in the presence of AgNPs 20 ppm and AgNPs 30 ppm when in suspensions with a density of 106 cells/mL. These results are promising for the successful use of AgNPs for disinfection, as well as for topical therapy of infections involving these bacterial species.

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Antibacterial and Antifungal Activity of Functionalized Cotton Fabric with Nanocomposite Based on Silver Nanoparticles and Carboxymethyl Chitosan

et al. 2022

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Cotton is the most widely used natural fiber for textiles; however, the capacity of cotton fibers to absorb large amounts of moisture, retain oxygen, and have a high specific surface area makes them more prone to microbial contamination, becoming an appropriate medium for the growth of bacteria and fungi. In recent years, the incorporation of silver nanoparticles in textile products has been widely used due to their broad-spectrum antibacterial activity and low toxicity towards mammalian cells. The aim of the current study is to continue the assessment of our developed nanocomposite and evaluate the antibacterial and antifungal activity of the nanocomposite based on silver nanoparticles and carboxymethyl chitosan (AgNPs-CMC) against Escherichia coli, Staphylococcus aureus, and Candida albicans, evaluated by the well diffusion method. The antibacterial activity against E. coli and S. aureus was also evaluated by the qualitative method of inhibition zone and the quantitative method of colony counting. Likewise, the antifungal activity of the functionalized fabric against Candida albicans and Aspergillus niger was determined by the inhibition zone method and the antifungal activity method GBT 24346-2009, respectively. The functionalized fabric showed 100% antibacterial activity against E. coli and S. aureus and good antifungal activity against C. albicans and A. niger. Our results indicate that the functionalized fabric could be used in garments for hospital use to reduce nosocomial infections.

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The study of the antimicrobial activity of colloidal solutions of silver nanoparticles prepared using food stabilizers

Cited by 3 publications

References 25 publications

Optimization of Synthesis of Silver Nanoparticles Conjugated with Lepechinia meyenii (Salvia) Using Plackett-Burman Design and Response Surface Methodology—Preliminary Antibacterial Activity

Optimization of Synthesis of Silver Nanoparticles Conjugated with Lepechinia meyenii (Salvia) Using Plackett-Burman Design and Response Surface Methodology—Preliminary Antibacterial Activity

In vitro antimicrobial activity of colloidal nano silver

Antibacterial and Antifungal Activity of Functionalized Cotton Fabric with Nanocomposite Based on Silver Nanoparticles and Carboxymethyl Chitosan

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