The study of the potential application of nanofiber microcapsules loading lactobacillus in targeted delivery of digestive tract in vitro

Chang, Xiaofeng; Lambo, Modinat Tolani; Dasen, Liu; Li, Xinglin

doi:10.1016/j.lwt.2021.111692

Cited by 12 publications

(7 citation statements)

References 46 publications

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“…ruminantium and Lactobacillus in ELAN was significantly lower ( p < 0.05). In our previous study, we established that the structural integrity of nanofibers was better at a high concentration of bile salt, which proved that the nanofibers had certain resistance to bile salt (Chang et al, 2021). It was worth noting that ELAN could improve the relative abundance of L .…”

Section: Resultsmentioning

confidence: 94%

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Nanofiber could deliver lactic acid bacteria to the intestine of ruminant in vitro experiment

Chang

Dasen

Lambo

2021

Animal Physiology Nutrition

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This study investigates the use of nanofiber microcapsules produced by electrostatic spinning as a carrier for the delivery of lactic acid bacteria (LAB) to the intestine of ruminants. We hypothesized that the LAB encapsulated into nanofiber microcapsules can be delivered to a ruminant's intestinal tract with little effect on the rumen fermentation and related bacteria. The in vitro experiment included three treatments: control group; 0.01g Lactobacillus acidophilus NCFM (L. acidophilus NCFM) encapsulated in nanofiber microcapsules by electrostatic spinning group (ELAN, 2.0 × 1011 CFU/g); and 0.01g L. acidophilus NCFM powder group (LANP, 2.0 × 1011 CFU/g), each incubated with 30 ml of buffer rumen fluid for 48h to determine the effect on rumen fermentation, then the abundance of L. acidophilus NCFM in the intestine was estimated using the modified in vitro three‐step procedure. Treatment responses were statistically analysed using one‐way ANOVA. The results showed that compared to the control, the ELAN group had a significant increase in pH (p < 0.05), while the LANP group had a non‐significant decrease in pH (p > 0.05). LANP and ELAN groups had no significant influence on total volatile fatty acid and individual volatile fatty acids (p > 0.05), apart from isobutyric acid of both groups, which reduced (p < 0.05). ELAN group had a decreasing trend of gas production and dry matter digestion, while the LANP group increased them significantly (p < 0.05). During the 16h and 48h rumen incubation, compared with control, there was no significant change in all bacteria in the ELAN group (p > 0.05), while the LANP group increased the relative abundance levels of S. bovis, S. ruminantium, M. elsdenii, F. succinogenes, B. fibrisolvens, Lactobacillus, L. acidophilus NCFM (p < 0.05). In the intestinal part, compared with control, the relative abundance of L. acidophilus NCFM in the ELAN group increased significantly (p < 0.05), while the result was not observed in the LANP group. We concluded based on our findings that L. acidophilus NCFM could be protected by nanofiber microcapsules and delivered to the intestinal site with little influence on the rumen fermentation and bacterial community, suggesting nanofiber microcapsules prepared by electrospinning technology could be used as a carrier for rumen‐protected study.

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Section: Resultsmentioning

confidence: 94%

“…The nanofiber microcapsules loading Lactobacillus acidophilus NCFM ( L . acidophilus NCFM) used in the study were prepared as described in our previous study (Chang et al, 2021). Lactobacillus acidophilus NCFM powder (ATCC, NO.…”

Section: Methodsmentioning

confidence: 99%

Nanofiber could deliver lactic acid bacteria to the intestine of ruminant in vitro experiment

Chang

Dasen

Lambo

2021

Animal Physiology Nutrition

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“…Specifically, the elevation in applied voltage causes the compound to form more jets, and therefore there was a clear trend of the diameter increasing. 36,39 Figures 3(c) and (d) visualize the interaction between air pressure and winding speed, in which we note that the interaction significantly affects the yarn diameter. Yarn diameter is negatively correlated with air pressure, and more notably with winding speed.…”

Section: Response Surface Interaction Analysismentioning

confidence: 99%

Optimization of the preparation process of electrostatic-solution blow spinning nanofiber yarn using response surface methodology

Shi

Wang

Zhang

et al. 2022

Textile Research Journal

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There are several methods of preparing nanofiber yarns, but little work has been reported using a combination of electrospinning and solution blowing methods (i.e., electrostatic-solution blow spinning) to prepare nanofiber yarns. In this study, a response surface method (RSM) based on Box–Behnken design was used to investigate the relationship between the yarn parameters and yarn diameter, and the process was optimized. The yarn diameter was simulated by electrostatic voltage, air pressure, winding speed and funnel velocity. The correlation coefficients before and after adjustment were 0.97 and 0.95, respectively, through variance analysis, which verified the accuracy of the yarn diameter model. The results showed that the influence of each experimental factor on the yarn diameter was in descending order of winding speed, electrostatic voltage, airflow and funnel velocity, and there were interactions among the factors. The BDD response model was used to obtain the best experimental conditions, which provided a good theoretical reference for the preparation of polyacrylonitrile nanofibers. In addition, the effects of voltage and air pressure on the diameter of the nanofiber and yarn and the effects of the twist coefficient on the shape and mechanical properties of the yarn were studied. The results showed that with the increase of voltage and air pressure, the diameter of the nanofiber decreased, and the yarn diameter decreased first and then increased. When the twist factor was 100, the twist angle of the yarn was 49.91°, the breaking strength was 12.92 MPa and the elongation at break was 81.34%.

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“…Some authors have included inactivated microorganisms, describing them as "live or dead bacteria, or components of bacteria (such as cell walls) that work under multiple modes of action, conferring positive effects to the administered animal or its environment" [20]. Before registering a strain as probiotic, specific criteria must be ensured and documented, such as its ability to survive and be preserved in the digestive tracts during the passage [21], nonpathogenicity and toxicity, lack of undesirable side effects, stability, large-scale production potential, and beneficial clinical effects on the administered animals [22][23][24]. Potential candidates should be able to modify specific physiological parameters or the immune system, attenuate pathogens, treat and prevent infections, inflammation, and disease while also acting as a biological control to prevent spoilage [22,25].…”

Section: Common Probiotic Strains and Their Mode Of Actionmentioning

confidence: 99%

The Recent Trend in the Use of Multistrain Probiotics in Livestock Production: An Overview

Lambo

Chang

Dasen

2021

Animals

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It has been established that introducing feed additives to livestock, either nutritional or non-nutritional, is beneficial in manipulating the microbial ecosystem to maintain a balance in the gut microbes and thereby improving nutrient utilization, productivity, and health status of animals. Probiotic use has gained popularity in the livestock industry, especially since antimicrobial growth promoter’s use has been restricted due to the challenge of antibiotic resistance in both animals and consumers of animal products. Their usage has been linked to intestinal microbial balance and improved performance in administered animals. Even though monostrain probiotics could be beneficial, multistrain probiotics containing two or more species or strains have gained considerable attention. Combining different strains has presumably achieved several health benefits over single strains due to individual isolates’ addition and positive synergistic adhesion effects on animal health and performance. However, there has been inconsistency in the effects of the probiotic complexes in literature. This review discusses multistrain probiotics, summarizes selected literature on their effects on ruminants, poultry, and swine productivity and the various modes by which they function.

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The study of the potential application of nanofiber microcapsules loading lactobacillus in targeted delivery of digestive tract in vitro

Cited by 12 publications

References 46 publications

Nanofiber could deliver lactic acid bacteria to the intestine of ruminant in vitro experiment

Nanofiber could deliver lactic acid bacteria to the intestine of ruminant in vitro experiment

Optimization of the preparation process of electrostatic-solution blow spinning nanofiber yarn using response surface methodology

The Recent Trend in the Use of Multistrain Probiotics in Livestock Production: An Overview

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