The study of toluidine blue-ribosomal RNA complexes

Investigation on the toxic interaction of toluidine blue with calf thymus DNA

“…Popa and coworkers [9,19] studied ribonucleic acid-TB complexes by gel electrophoresis and spectrophotometry and obtained a similar conclusion. They found that at low dye concentrations, natural RNA and TB form the so-called Complex I, in which intercalation of TB between Watson and Crick type base pairs is assumed to occur.…”

Studies on Isolated Aggregating Oligoribonucleoproteins of the Epidermis With Histochemical and Morphological Characteristics of Keratohyalin

“…Recovery of crude oligomer protein ranged from 6 .88 X 10-3 % to 8 the nucleic acid or the nucleic acid itself (1,18,19,33,49) . In the case of toluidine blue, such binding would result in the alcohol-stable ß-metachromasia noted with keratohyalin granules (18,19) AsTxux R. UGEL Aggregating Oligoribonuçlepproteins of the Epidermis FIGURE 14 Fractionation of crude oligomers on a 9% T/3% C polyacrylamide gel stained with amido black.…”

Kinetic Studies of the Interaction of Toluidine Blue with Poly-(αL-glutamic acid) by Means of Stopped-Flow Method

“…The course of the reaction was monitored at 635 nm which was due to the absorption of toluidine blue monomer. 16 The range of the total concentration of (Glu)n (cp 0 ) was 2-40 X 10-5 M. In the experiments when the P/D is varied, the dye concentration was fixed at 2 x 10-5 M (after mixing) and the (Glu)n concentration was increased, that is, the P/D was varied up to 20. Some experiments were also carried out in which the concentrations of both the (Glu)n and the toluidine blue were varied at a constant P/D.…”