The study on the interaction between phytosterols and phospholipids in model membranes

Hąc-Wydro, Katarzyna; Wydro, Paweł; Jagoda, Agnieszka; Kapusta, Joanna

doi:10.1016/j.chemphyslip.2007.06.211

Cited by 88 publications

(69 citation statements)

References 32 publications

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“…Comparable ability of cholesterol and b-sitosterol to increase the bilayer thickness of unilamellar vesicles was also observed in our recent reports on DMPC and DPPC bilayers (Gallová et al 2011) and diCn:1PC (1,2-monounsaturated diacyl-sn-glycero-3-phosphatidylcholine with n carbon atoms in the acyl chain) bilayers (n = 14-22) (Gallová et al 2008). A smaller ability of b-sitosterol in comparison to cholesterol to increase the thickness of hydrated phosphatidylcholine bilayers was reported by other techniques (Hodzic et al 2008;Krajewski-Bertrand et al 1992;Bernsdorff and Winter 2003;Hac-Wydro et al 2007). Such differences between the effects of cholesterol and b-sitosterol on d B were not observed in our measurements, although the limited accuracy of the SANS method, approximately 0.2 nm for determination of d B (Fig.…”

Section: Resultsmentioning

confidence: 52%

Influence of Cholesterol and β-Sitosterol on the Structure of EYPC Bilayers

et al. 2011

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The influence of cholesterol and b-sitosterol on egg yolk phosphatidylcholine (EYPC) bilayers is compared. Different interactions of these sterols with EYPC bilayers were observed using X-ray diffraction. Cholesterol was miscible with EYPC in the studied concentration range (0-50 mol%), but crystallization of b-sitosterol in EYPC bilayers was observed at X C 41 mol% as detected by X-ray diffraction. Moreover, the repeat distance (d) of the lamellar phase was similar upon addition of the two sterols up to mole fraction 17%, while for X C 17 mol% it became higher in the presence of b-sitosterol compared to cholesterol. SANS data on suspensions of unilamellar vesicles showed that both cholesterol and b-sitosterol similarly increase the EYPC bilayer thickness. Cholesterol in amounts above 33 mol% decreased the interlamellar water layer thickness, probably due to ''stiffening'' of the bilayer. This effect was not manifested by b-sitosterol, in particular due to the lower solubility of b-sitosterol in EYPC bilayers. Applying the formalism of partial molecular areas, it is shown that the condensing effect of both sterols on the EYPC area at the lipid-water interface is small, if any. The parameters of ESR spectra of spin labels localized in different regions of the EYPC bilayer did not reveal any differences between the effects of cholesterol and b-sitosterol in the range of full miscibility.

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Section: Resultsmentioning

confidence: 52%

Influence of Cholesterol and β-Sitosterol on the Structure of EYPC Bilayers

et al. 2011

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“…b-Sitosterol (Fig. 1b) is one of the most abundant plant sterols (reviewed by Hac-Wydro et al 2007). It differs structurally from cholesterol only in the ethyl substituent at the C24 carbon of the side chain.…”

Section: Introductionmentioning

confidence: 99%

The effects of cholesterol and β-sitosterol on the structure of saturated diacylphosphatidylcholine bilayers

et al. 2010

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“…They are principally localized to cytoplasmic or endomembranes, although the latter are composed predominantly of phospholipids. Sterols participate in membrane permeability and rigidity, in the formation of lipid rafts and, eventually, in post-translational control of membrane proteins (Hac-Wydro et al, 2007;Yeagle, 1990;Epand, 2008;Gilbert, 2010;Paila & Chattopadhyay, 2010). The major fungal sterol is ergosterol and specific to this kingdom.…”

Section: Fenhexamid: a Single Mode Of Actionmentioning

confidence: 99%