Poly(vinyl alcohol) (PVA) thin films with different amounts of silver (Ag) nanoparticles (0.001, 0.002, 0.003, 0.004 and 0.005)g were created using the solution cast method. PVA/Ag nanocomposite structural and optical characteristics have been investigated. Using the X-ray Diffraction (XRD) technique, the produced nanocomposite's structural analysis was conducted.The Wurtzite structure of Ag nanoparticles was confirmed by the XRD pattern. The chemical bond and stretching vibrations attributed to different peaks were evaluated using FTIR spectra.UV-Vis spectroscopy has been used to investigate the impact of AgNPs on the optical properties of PVA, including absorbance, transmittance, absorption coefficient, refractive index, extinction coefficient, and real and imaginary components of the dielectric constant. the energy gap decreases the most plausible reason is the creation of a localized level in the forbidden energy gap as a result of increasing the fillers nanoparticle weight. This study shows that all of these characteristics are affected by the number of AgNPs. , the antimicrobial activity of nanocomposite must be recorded. Hence, positive bacteria (Staphylococcus aureus, Streptococcus pneumonia), negative bacteria (Escherichia coli, Pseudomonas aeruginosa), and fungus (Candida albicans) were taken as models in this work. The maximum inhibitaion zone zones for PEG+(0.005g) Ag in Candida albicans and Staphylococcus aureus were about 18 mm, respectively.